This HTML5 document contains 929 embedded RDF statements represented using HTML+Microdata notation.

The embedded RDF content will be recognized by any processor of HTML5 Microdata.

Namespace Prefixes

PrefixIRI
rdfshttp://www.w3.org/2000/01/rdf-schema#
bibohttp://purl.org/ontology/bibo/
n8http://pubannotation.org/docs/sourcedb/PMC/sourceid/
n11http://togows.dbcls.jp/entry/pubmed/
xsdhhttp://www.w3.org/2001/XMLSchema#
n3http://purl.jp/bio/10/colil/id/
n9http://dx.doi.org/
n2http://purl.jp/bio/10/colil/ontology/201303#
n10http://www.ncbi.nlm.nih.gov/pmc/articles/
n4http://purl.org/spar/doco/
dchttp://purl.org/dc/elements/1.1/
rdfhttp://www.w3.org/1999/02/22-rdf-syntax-ns#

Statements

Subject Item
n3:21978405
rdf:type
n2:RelevantPaper n2:ReferencePaper n2:CitationPaper
rdfs:seeAlso
n8:0 n9:10.1186%2F1742-2094-8-131 n10:0 n11:21978405
bibo:cites
n3:17112751 n3:18173372 n3:15939794 n3:16799470 n3:9498303 n3:12876559 n3:17171761 n3:10993917 n3:10760787 n3:11022932 n3:17998915 n3:14636780 n3:19829296 n3:1375472 n3:8600538 n3:11307161 n3:19179377 n3:7668836 n3:19167248 n3:16580261 n3:20159168 n3:1677055 n3:14595363 n3:17721537 n3:15735651 n3:16955140 n3:16708400 n3:18711433 n3:20870176 n3:7525854 n3:12016203 n3:17259991 n3:15831717 n3:17878332 n3:20167381 n3:11086982 n3:11877483 n3:7848517
n2:cocitationWith
n3:20637861 n3:9221750 n3:25059097 n3:14523098 n3:21166679 n3:23435332 n3:27493626 n3:30061532 n3:17717539 n3:24399752 n3:23448240 n3:18173372 n3:30647813 n3:19144568 n3:18278054 n3:25268136 n3:15379901 n3:25152710 n3:12225888 n3:10993917 n3:29428213 n3:29759483 n3:10760787 n3:22727420 n3:30128145 n3:20134419 n3:30389931 n3:12876559 n3:18573909 n3:27098648 n3:19339593 n3:28839214 n3:29078813 n3:18579442 n3:12871638 n3:8371781 n3:18792018 n3:14684867 n3:20440079 n3:15944226 n3:15368284 n3:29774058 n3:18848864 n3:16393993 n3:20332793 n3:22896675 n3:26101267 n3:11745370 n3:19829296 n3:15543150 n3:22293174 n3:19444307 n3:19179377 n3:20623124 n3:19836266 n3:18496841 n3:17709958 n3:7937744 n3:23598650 n3:14712275 n3:8359494 n3:26876939 n3:19167248 n3:15349858 n3:24556681 n3:20623286 n3:24736453 n3:22131937 n3:23920495 n3:2321027 n3:26431936 n3:27229916 n3:10854289 n3:15895084 n3:20566674 n3:17114485 n3:12504866 n3:27802243 n3:22722867 n3:16708400 n3:15735651 n3:22296715 n3:23529826 n3:25352786 n3:29608988 n3:20605228 n3:12016203 n3:21235577 n3:18362179 n3:23452861 n3:27616557 n3:20812720 n3:12615204 n3:16299478 n3:18711433 n3:20870176 n3:14565534 n3:16818754 n3:26441969 n3:26964686 n3:23571678 n3:27702698 n3:15831717 n3:23589621 n3:17417634 n3:12415310 n3:15928201 n3:14668803 n3:16964261 n3:12576233 n3:19896530 n3:29545515 n3:26255669 n3:20723761 n3:26669439 n3:29917120 n3:23219269 n3:22341445 n3:23054369 n3:12781128 n3:21919736 n3:8811196 n3:28283756 n3:23337699 n3:30186112 n3:21239728
n2:hasRelevantBibliographicResourceOf
_:vb474386544 _:vb474386545 _:vb474386546 _:vb474386547 _:vb474386548 _:vb474386549 _:vb474386550 _:vb474386551 _:vb474386552 _:vb474386553 _:vb474386554 _:vb474386555 _:vb474386556 _:vb474386557 _:vb474386558 _:vb474386559 _:vb474386531 _:vb474386532 _:vb474386533 _:vb474386534 _:vb474386535 _:vb474386536 _:vb474386537 _:vb474386538 _:vb474386539 _:vb474386540 _:vb474386541 _:vb474386542 _:vb474386543 _:vb474386608 _:vb474386609 _:vb474386610 _:vb474386611 _:vb474386612 _:vb474386613 _:vb474386614 _:vb474386615 _:vb474386616 _:vb474386617 _:vb474386618 _:vb474386619 _:vb474386620 _:vb474386621 _:vb474386622 _:vb474386623 _:vb474386592 _:vb474386593 _:vb474386594 _:vb474386595 _:vb474386596 _:vb474386597 _:vb474386598 _:vb474386599 _:vb474386600 _:vb474386601 _:vb474386602 _:vb474386603 _:vb474386604 _:vb474386605 _:vb474386606 _:vb474386607 _:vb474386576 _:vb474386577 _:vb474386578 _:vb474386579 _:vb474386580 _:vb474386581 _:vb474386582 _:vb474386583 _:vb474386584 _:vb474386585 _:vb474386586 _:vb474386587 _:vb474386588 _:vb474386589 _:vb474386590 _:vb474386591 _:vb474386560 _:vb474386561 _:vb474386562 _:vb474386563 _:vb474386564 _:vb474386565 _:vb474386566 _:vb474386567 _:vb474386568 _:vb474386569 _:vb474386570 _:vb474386571 _:vb474386572 _:vb474386573 _:vb474386574 _:vb474386575 _:vb474386656 _:vb474386640 _:vb474386641 _:vb474386642 _:vb474386643 _:vb474386644 _:vb474386645 _:vb474386646 _:vb474386647 _:vb474386648 _:vb474386649 _:vb474386650 _:vb474386651 _:vb474386652 _:vb474386653 _:vb474386654 _:vb474386655 _:vb474386624 _:vb474386625 _:vb474386626 _:vb474386627 _:vb474386628 _:vb474386629 _:vb474386630 _:vb474386631 _:vb474386632 _:vb474386633 _:vb474386634 _:vb474386635 _:vb474386636 _:vb474386637 _:vb474386638 _:vb474386639
n2:pmcid
PMC0
bibo:doi
10.1186%2F1742-2094-8-131
n4:contains
_:vb12797007 _:vb12797015 _:vb12797029 _:vb12797048 _:vb12796996
Subject Item
_:vb12796996
rdf:type
n4:Section
dc:title
results
n4:contains
_:vb12796997 _:vb12796998 _:vb12796999 _:vb12797004 _:vb12797005 _:vb12797006 _:vb12797000 _:vb12797001 _:vb12797002 _:vb12797003
Subject Item
_:vb12796997
rdf:type
n2:Context
rdf:value
Whereas we previously observed no lymphocytes in healthy mice deep in the CNS parenchyma we could detect some sporadic cells in the perivascular space [>>3<<]. However, during EAE naïve T cells were able to invade the parenchyma after local application on the brainstem or to cross the corrupted blood brain barrier after IV transfer and to move rapidly (Figure 1B, Additional File 1) resulting
n2:mentions
n3:19179377
Subject Item
_:vb12796998
rdf:type
n2:Context
rdf:value
The dynamic interaction between both cell types was examined by co-localisation analysis as previously described [>>19<<]. We discriminated between short (random) contacts and long-lasting (most probably non-random). On their way through the parenchyma 19% of all contacts were of long-lasting nature whereas the majority (81 ± 14%) of the naïve-effector T
n2:mentions
n3:20870176
Subject Item
_:vb12796999
rdf:type
n2:Context
rdf:value
(E) To quantify effector-naïve T cell interactions we analyse the co-localisation area of EGFP and tdRFP as previously described [>>19<<]. We discriminated short (random) contacts (< 5 min) from long-lasting (most probably non-random) interactions (≥ 5 min) and observed that 81% ± 14 formed short interactions with effector T cells (white bar) and 19% ± 14 (gray bar) formed
n2:mentions
n3:20870176
Subject Item
_:vb12797000
rdf:type
n2:Context
rdf:value
As it was not possible to investigate the behaviour of naïve CD4+ T cells in healthy living anaesthetized mice due to the low frequency of lymphocytes after IV transfer [>>21<<], we circumvented the blood brain barrier and applied naive CD4 T cells on the brain stem of healthy living anaesthetized mice.
n2:mentions
n3:15939794
Subject Item
_:vb12797001
rdf:type
n2:Context
rdf:value
These CNS slices have been shown to retain a middle layer which consists of intact in vivo-like CNS in murine brain cultures [>>22<<]. Naïve CD4+ T cells were labeled with the Celltracker Orange 5-(and-6)-(4-chloromethyl(benzoyl)amino) tetramethylrhodamine (CMTMR) if they were not transgenic for tdRFP or EGFP, and consecutively co-incubated with a living brain slice in
n2:mentions
n3:11022932
Subject Item
_:vb12797002
rdf:type
n2:Context
rdf:value
We previously showed that CD4+ antigen specific, highly-activated and differentiated effector T cell lines show a vessel-associated movement pattern [>>3<<]. We wanted to check whether antigen specificity or regulatory phenotype would influence T cell migration. Therefore we performed T cell brain slice co-culture experiments as described above exemplarily for activated antigen specific (OT2
n2:mentions
n3:19179377
Subject Item
_:vb12797003
rdf:type
n2:Context
rdf:value
The vessel-associated movement of CD4+ T cells in contrast to CD8+ T cells [>>3<<] seems also to be important for regulatory T cell subtypes independent of antigen specificity and underlines the importance of the perivascular space for immunoregulation and autoimmunity.
n2:mentions
n3:19179377
Subject Item
_:vb12797004
rdf:type
n2:Context
rdf:value
Cell tracking analysis of individual T cells confirmed our qualitative observations, as the mean track velocity with 0.17 ± 0.06 μm/sec (± SD; N = 126) was similar to what was previously described by others [>>2<<] (Figure 4C).
n2:mentions
n3:12016203
Subject Item
_:vb12797005
rdf:type
n2:Context
rdf:value
In lymphoid tissue it is known that naïve T cell motility is directed by fibroblastic reticular cells and ECM structures which can be visualised by second harmonic generation (SHG) [>>23<<]. Interestingly, we observed similar SHG signals which were generated by an optical parametric oscillator (OPO) at 1110 nm wavelength in the inflamed CNS of EAE affected mice.
n2:mentions
n3:17112751
Subject Item
_:vb12797006
rdf:type
n2:Context
rdf:value
intravital TPLSM on the brain stem of EAE affected C57BL/6 Thy1.1 CerTN L15 mice which express a FRET based calcium sensor, i.e. the Citrulin/Cerulean FRET pair (based on YFP/CFP) in neurons and neuronal processes (axons and dendrites) [>>17<<], we could distinguish the generated SHG signal due to wavelength specificity of SHG (1110 nm) and separated excitation of Citrulin (850 nm).
n2:mentions
n3:17259991
Subject Item
_:vb12797007
rdf:type
n4:Section
dc:title
conclusions
n4:contains
_:vb12797008 _:vb12797009 _:vb12797010 _:vb12797011 _:vb12797012 _:vb12797013 _:vb12797014
Subject Item
_:vb12797008
rdf:type
n2:Context
rdf:value
The mechanisms of autoimmunity and tolerance in the brain are still not clear and the role of naïve T cells as possible key players in these complex processes is highly debated [>>33<<]. Due to methodological limitations in the past, there remains an ongoing discussion as to what extent naïve T cells enter the healthy non-inflamed versus the inflamed CNS parenchyma and what role they might have.
n2:mentions
n3:17998915
Subject Item
_:vb12797009
rdf:type
n2:Context
rdf:value
For activated T cells, proteolytic enzymes like metalloproteinases, disintegrins or granzymes are known to enable migration through the inflamed brain tissue [>>12<<,34,35]. These enzymes may be even more important if the reticular fiber network is not as abundant, as it is the case under inflammatory conditions where it facilitates immune cell trafficking. In contrast naïve T cells express molecules
n2:mentions
n3:1677055
Subject Item
_:vb12797010
rdf:type
n2:Context
rdf:value
For activated T cells, proteolytic enzymes like metalloproteinases, disintegrins or granzymes are known to enable migration through the inflamed brain tissue [12,>>34<<,35]. These enzymes may be even more important if the reticular fiber network is not as abundant, as it is the case under inflammatory conditions where it facilitates immune cell trafficking. In contrast naïve T cells express molecules
n2:mentions
n3:14636780
Subject Item
_:vb12797011
rdf:type
n2:Context
rdf:value
For activated T cells, proteolytic enzymes like metalloproteinases, disintegrins or granzymes are known to enable migration through the inflamed brain tissue [12,34,>>35<<]. These enzymes may be even more important if the reticular fiber network is not as abundant, as it is the case under inflammatory conditions where it facilitates immune cell trafficking. In contrast naïve T cells express molecules that
n2:mentions
n3:9498303
Subject Item
_:vb12797012
rdf:type
n2:Context
rdf:value
In contrast naïve T cells express molecules that are important for migration to secondary lymphoid organs such as L-Selectin and CCR7 for CD4+ T cells [>>36<<]. So it was previously thought that they circulate between blood and secondary lymphoid organs exclusively [37,38].
n2:mentions
n3:7525854
Subject Item
_:vb12797013
rdf:type
n2:Context
rdf:value
So it was previously thought that they circulate between blood and secondary lymphoid organs exclusively [>>37<<,38].
n2:mentions
n3:16580261
Subject Item
_:vb12797014
rdf:type
n2:Context
rdf:value
So it was previously thought that they circulate between blood and secondary lymphoid organs exclusively [37,>>38<<].
n2:mentions
n3:8600538
Subject Item
_:vb12797015
rdf:type
n4:Section
dc:title
methods
n4:contains
_:vb12797024 _:vb12797025 _:vb12797026 _:vb12797027 _:vb12797028 _:vb12797016 _:vb12797017 _:vb12797018 _:vb12797019 _:vb12797020 _:vb12797021 _:vb12797022 _:vb12797023
Subject Item
_:vb12797016
rdf:type
n2:Context
rdf:value
C57BL/6 OT2 mice, C57BL/6 2d2 TCR [>>14<<] transgenic mice (kindly provided by A.
n2:mentions
n3:16955140
Subject Item
_:vb12797017
rdf:type
n2:Context
rdf:value
Waismann), C57BL/6 Rosa26 tdRFP ("ΔNeo-flip")mice [>>15<<], obtained from H.J. Fehling, or C57BL/6 β-actin-EGFP transgenic mice were intercrossed to generate C57BL/6 OT2 tdRFP, C57BL/6 2d2 tdRFP mice and C57BL/6 OT2 EGFP mice, respectively.
n2:mentions
n3:17171761
Subject Item
_:vb12797018
rdf:type
n2:Context
rdf:value
C57BL/6 Thy1-21 EGFP [>>16<<] mice were kindly provided by P. Caroni, C57BL/6 Thy1.1 CerTN L15 mice were kindly provided by O.
n2:mentions
n3:11086982
Subject Item
_:vb12797019
rdf:type
n2:Context
rdf:value
Caroni, C57BL/6 Thy1.1 CerTN L15 mice were kindly provided by O.Griesbeck [>>17<<] C57BL/6 Foxp3 EGFP mice were kindly provided by B. and M.
n2:mentions
n3:17259991
Subject Item
_:vb12797020
rdf:type
n2:Context
rdf:value
Mice (6-10 weeks old) were sacrificed and spleen cells were isolated as described before [>>3<<]. For OT-2 Th2 cells splenocytes were cultured in 3 × 106/ml cell culture medium (RPMI 1640 supplemented with 2 mM L-glutamine, 100 U/ml penicillin, 100 μg/ml streptomycin, and 10% fetal calf serum), and stimulated with the respective
n2:mentions
n3:19179377
Subject Item
_:vb12797021
rdf:type
n2:Context
rdf:value
IL-10 producing Ovalbumin- or MOG-specific regulatory T cells were generated according to the protocol by Barrat et al.[>>18<<]. Briefly, naïve CD4+CD62L+T cells were stimulated with irradiated syngenic CD90depleted splenic APCs (MACS®) and 0.3 μM OVA323-339 or 12,5 μg/ml MOG35-55 peptide in the presence of 40 nM Vitamin D3 and 100 nM Dexamethasone and 5 μg/ml
n2:mentions
n3:11877483
Subject Item
_:vb12797022
rdf:type
n2:Context
rdf:value
EGFP mice were isolated and stimulated as previously described [>>19<<]. Three days after the second restimulation, 5 × 106 2d2.
n2:mentions
n3:20870176
Subject Item
_:vb12797023
rdf:type
n2:Context
rdf:value
T cells and vessels were visualised by a two-photon laser scanning system (LaVision BioTec, Bielefeld) as described before [>>20<<] with dual NIR (850 nm) and IR (1,110 nm) excitation. XYZ-stacks were typically collected within a scan field of 300 × 300 μm at 512 × 512 pixel resolution and a z-plane distance of 2 μm at a frequency of 400 or 800 Hz.
n2:mentions
n3:20159168
Subject Item
_:vb12797024
rdf:type
n2:Context
rdf:value
Preparation of brain slices was performed as previously described [>>3<<] and allowed to recover for at least 45 min at room temperature prior to transfer to a heated and with aerated artificial cerebrospinal fluid (ACSF) perfused Luigs & Neumann slice chamber (37°C).
n2:mentions
n3:19179377
Subject Item
_:vb12797025
rdf:type
n2:Context
rdf:value
For the brainstem imaging, the anaesthetized animal was transferred to a custom-built microscopy table and fixed in a hanging position. The preparation of the imaging field was performed as previously described [>>3<<].
n2:mentions
n3:19179377
Subject Item
_:vb12797026
rdf:type
n2:Context
rdf:value
Lymph node preparation was performed as previously described [>>20<<]. In brief, 8-96 hours after intravenous injection of naïve OT2 EGFP or OT2 tdRFP T cells, popliteal lymph nodes were removed, glued onto a coverslip and placed into a heated chamber at 36°C, superfused RPMI medium without phenol red
n2:mentions
n3:20159168
Subject Item
_:vb12797027
rdf:type
n2:Context
rdf:value
The co-localisation area was calculated with Volocity as previously described [>>19<<]. To discriminate short/transient (random) contacts from long-lasting (most probably non-random) interactions, we defined contacts which last longer than 5 min as 'long-lasting'. These interactions were further differentiated into
n2:mentions
n3:20870176
Subject Item
_:vb12797028
rdf:type
n2:Context
rdf:value
For angle comparison between cell trajectories and vessel structures and for vessel distance measurements at the end of the track, cell-tracking data from Volocity was further processed in MATLAB as described previously [>>3<<]. For each track, a vector between origin and endpoint was calculated in a two-dimensional angle and the distance between the cell-tracking vector and the vector representing the vessel was calculated using standard linear algebra.
n2:mentions
n3:19179377
Subject Item
_:vb12797029
rdf:type
n4:Section
dc:title
discussion
n4:contains
_:vb12797040 _:vb12797041 _:vb12797042 _:vb12797043 _:vb12797044 _:vb12797045 _:vb12797046 _:vb12797047 _:vb12797030 _:vb12797031 _:vb12797032 _:vb12797033 _:vb12797034 _:vb12797035 _:vb12797036 _:vb12797037 _:vb12797038 _:vb12797039
Subject Item
_:vb12797030
rdf:type
n2:Context
rdf:value
Regarding the CNS even less is known and most of the data are derived from flow cytometry studies of cells isolated from CNS tissue [>>8<<,9,13,24,25] or cerebrospinal fluid [26] and give contradictory results.
n2:mentions
n3:16708400
Subject Item
_:vb12797031
rdf:type
n2:Context
rdf:value
Regarding the CNS even less is known and most of the data are derived from flow cytometry studies of cells isolated from CNS tissue [8,>>9<<,13,24,25] or cerebrospinal fluid [26] and give contradictory results.
n2:mentions
n3:10993917
Subject Item
_:vb12797032
rdf:type
n2:Context
rdf:value
Regarding the CNS even less is known and most of the data are derived from flow cytometry studies of cells isolated from CNS tissue [8,9,>>13<<,24,25] or cerebrospinal fluid [26] and give contradictory results.
n2:mentions
n3:10760787
Subject Item
_:vb12797033
rdf:type
n2:Context
rdf:value
Regarding the CNS even less is known and most of the data are derived from flow cytometry studies of cells isolated from CNS tissue [8,9,13,>>24<<,25] or cerebrospinal fluid [26] and give contradictory results.
n2:mentions
n3:20167381
Subject Item
_:vb12797034
rdf:type
n2:Context
rdf:value
Regarding the CNS even less is known and most of the data are derived from flow cytometry studies of cells isolated from CNS tissue [8,9,13,24,>>25<<] or cerebrospinal fluid [26] and give contradictory results.
n2:mentions
n3:15735651
Subject Item
_:vb12797035
rdf:type
n2:Context
rdf:value
Regarding the CNS even less is known and most of the data are derived from flow cytometry studies of cells isolated from CNS tissue [8,9,13,24,25] or cerebrospinal fluid [>>26<<] and give contradictory results.
n2:mentions
n3:7668836
Subject Item
_:vb12797036
rdf:type
n2:Context
rdf:value
Using an in vivo staining method, a recent study did not detect naïve CD4+ T cells in the brain at 4 days after transfer of naïve T cells [>>24<<]. In contrast experiments using an inverse transfer model (encephalitogenic cells into OVA specific T cell receptor transgenic mice) show that naïve endogenous T cells occur in parallel with activated encephalitogenic T cells but keep a
n2:mentions
n3:20167381
Subject Item
_:vb12797037
rdf:type
n2:Context
rdf:value
cells into OVA specific T cell receptor transgenic mice) show that naïve endogenous T cells occur in parallel with activated encephalitogenic T cells but keep a resting phenotype unless they recognize their antigen in the CNS [>>13<<]. Similarly, McMahon et al. could only detect naïve PLP specific T cells in PLP immunized mice in contrast to OVA immunized controls.
n2:mentions
n3:10760787
Subject Item
_:vb12797038
rdf:type
n2:Context
rdf:value
This supposes that naïve T cells can only enter the CNS if they get locally activated [>>25<<].
n2:mentions
n3:15735651
Subject Item
_:vb12797039
rdf:type
n2:Context
rdf:value
In MS naïve T cells are found in the cerebrospinal fluid during relapses [>>26<<] and the experiments from Brabb et al. support a possible function for the development of tolerance [9]:
n2:mentions
n3:7668836
Subject Item
_:vb12797040
rdf:type
n2:Context
rdf:value
In MS naïve T cells are found in the cerebrospinal fluid during relapses [26] and the experiments from Brabb et al. support a possible function for the development of tolerance [>>9<<]: Using MBP (myelin basic protein)-recognizing T-cell receptor transgenic mice, they showed that naïve MBP-specific T cells isolated from the brain did not show any reactivity for MBP in vitro. On the other hand, MBP-specific naïve T
n2:mentions
n3:10993917
Subject Item
_:vb12797041
rdf:type
n2:Context
rdf:value
In lymphoid tissue it is known that T cell motility is directed by fibroblastic reticular cells and ECM structures [>>23<<]. Similar reticular structures exist in peripheral tissues such as the liver or skin, but are absent from the non-inflamed brain [27-29]. The source of the ECM structures in the CNS during inflammatory conditions we could detect in this
n2:mentions
n3:17112751
Subject Item
_:vb12797042
rdf:type
n2:Context
rdf:value
Similar reticular structures exist in peripheral tissues such as the liver or skin, but are absent from the non-inflamed brain [>>27<<-29]. The source of the ECM structures in the CNS during inflammatory conditions we could detect in this study, however, is unclear. The ECM seems to undergo significant structural changes in the CNS since we could only detect SHG
n2:mentions
n3:17878332 n3:19167248 n3:18711433
Subject Item
_:vb12797043
rdf:type
n2:Context
rdf:value
Similarly, Wilson et al. showed a reticular fiber network the produces a SHG signal during Toxoplasma gondii infection [>>29<<]. SHG generated by excitation at wavelengths of 1000-1500 nm in TPLSM has its origin from highly-ordered, non-centrosymmetric structures such as collagen [30]. As the SHG phenomenon gives no indication of the molecular nature of the
n2:mentions
n3:19167248
Subject Item
_:vb12797044
rdf:type
n2:Context
rdf:value
SHG generated by excitation at wavelengths of 1000-1500 nm in TPLSM has its origin from highly-ordered, non-centrosymmetric structures such as collagen [>>30<<]. As the SHG phenomenon gives no indication of the molecular nature of the structures, it remains unclear which fibers are generating this signal. In the study of Wilson et al. the SHG signal was co-localised with GFAP expressing
n2:mentions
n3:14595363
Subject Item
_:vb12797045
rdf:type
n2:Context
rdf:value
In the study of Wilson et al. the SHG signal was co-localised with GFAP expressing astrocytes, but with a spatial separation, suggesting that the SHG signal originates from extracellular fiber networks [>>29<<]. Furthermore, they found no increased collagen type IV or type I induction but reticular structures by histology which co-localised with CCL21 under inflammatory conditions, suggesting that CCL21 could be one possible candidate, because
n2:mentions
n3:19167248
Subject Item
_:vb12797046
rdf:type
n2:Context
rdf:value
but reticular structures by histology which co-localised with CCL21 under inflammatory conditions, suggesting that CCL21 could be one possible candidate, because it is also known that impaired neurons bind CCL21 on their surface [>>31<<]. However, as we investigated the co-localisation of neuronal structures and the SHG signal in EAE in transgenic mice expressing EGFP or Citrulin under the neuron specific Thy1 promoter, we could not detect any co-localisation.
n2:mentions
n3:11307161
Subject Item
_:vb12797047
rdf:type
n2:Context
rdf:value
matrix-bound CCL21 in an in vitro assay and showed that naïve T cells migrated only if the chemokine was matrix-bound [>>32<<]. Our experiments are consistent with these results and demonstrate in vivo relevance of the described phenomenon. Furthermore, our observations suggest that reticular fibers, analogous to those of the lymph node's ECM, may provide a
n2:mentions
n3:17721537
Subject Item
_:vb12797048
rdf:type
n4:Section
dc:title
background
n4:contains
_:vb12797052 _:vb12797053 _:vb12797054 _:vb12797055 _:vb12797049 _:vb12797050 _:vb12797051 _:vb12797056 _:vb12797057
Subject Item
_:vb12797049
rdf:type
n2:Context
rdf:value
In the last 10 years, two-photon laser scanning microscopy (TPLSM) has revealed the dynamic nature of immune cells within living lymphoid and target organs [>>1<<-6]. This has led not only to a better understanding of generation and priming of many immune cells, but also of the basics of immune regulation.
n2:mentions
n3:18173372 n3:16799470 n3:19829296 n3:12016203 n3:15831717 n3:19179377
Subject Item
_:vb12797050
rdf:type
n2:Context
rdf:value
activated CD4+ effector T cells are attracted to the CNS's perivascular space and reveal a CXCR4 dependent and vessel-associated migration pattern, suggesting this compartment is highly relevant for autoimmunity and immunoregulation [>>3<<,7]. While previous studies of ours as well as other studies mainly concentrated on T cells in their effector or effector-memory state, in the current study we focused on the behaviour of naïve and regulatory T cells.
n2:mentions
n3:19179377
Subject Item
_:vb12797051
rdf:type
n2:Context
rdf:value
activated CD4+ effector T cells are attracted to the CNS's perivascular space and reveal a CXCR4 dependent and vessel-associated migration pattern, suggesting this compartment is highly relevant for autoimmunity and immunoregulation [3,>>7<<]. While previous studies of ours as well as other studies mainly concentrated on T cells in their effector or effector-memory state, in the current study we focused on the behaviour of naïve and regulatory T cells.
n2:mentions
n3:12876559
Subject Item
_:vb12797052
rdf:type
n2:Context
rdf:value
Activated and memory T cells express adhesion molecules, chemokine receptors and integrins that enable them to cross the blood brain barrier to carry out immune surveillance of the CNS [>>7<<]. Adversely, naïve T cells which do not express essential proteolytic enzymes (e.g. matrix metalloproteinases) and adhesion ligands (e.g. LFA-1 and VLA-4), were thought to circulate only between the blood, lymph and secondary lymphoid
n2:mentions
n3:12876559
Subject Item
_:vb12797053
rdf:type
n2:Context
rdf:value
However, flow cytometry experiments showed that naïve T cells can indeed be found in the healthy, non-inflamed CNS [>>8<<,9]. This is also the case for other non-lymphoid tissues including the pancreas, intestine, lung, liver, kidney, skin and testis, where it is thought that this circulation is part of the normal migratory behaviour of naïve T cells [8].
n2:mentions
n3:16708400
Subject Item
_:vb12797054
rdf:type
n2:Context
rdf:value
However, flow cytometry experiments showed that naïve T cells can indeed be found in the healthy, non-inflamed CNS [8,>>9<<]. This is also the case for other non-lymphoid tissues including the pancreas, intestine, lung, liver, kidney, skin and testis, where it is thought that this circulation is part of the normal migratory behaviour of naïve T cells [8].
n2:mentions
n3:10993917
Subject Item
_:vb12797055
rdf:type
n2:Context
rdf:value
This is also the case for other non-lymphoid tissues including the pancreas, intestine, lung, liver, kidney, skin and testis, where it is thought that this circulation is part of the normal migratory behaviour of naïve T cells [>>8<<]. During CNS- inflammation adhesion ligands (i.e. ICAM-1 and VCAM) facilitate T cell recruitment to the CNS [10-12], and they only get activated when they encounter their antigen in the CNS [13].
n2:mentions
n3:16708400
Subject Item
_:vb12797056
rdf:type
n2:Context
rdf:value
During CNS- inflammation adhesion ligands (i.e. ICAM-1 and VCAM) facilitate T cell recruitment to the CNS [>>10<<-12], and they only get activated when they encounter their antigen in the CNS [13].
n2:mentions
n3:1677055 n3:1375472 n3:7848517
Subject Item
_:vb12797057
rdf:type
n2:Context
rdf:value
During CNS- inflammation adhesion ligands (i.e. ICAM-1 and VCAM) facilitate T cell recruitment to the CNS [10-12], and they only get activated when they encounter their antigen in the CNS [>>13<<].
n2:mentions
n3:10760787
Subject Item
_:vb474386531
rdf:type
n2:RelevantBibliographicResource
n2:RelevantScore
3
n2:hasRelevantPaperId
n3:16708400
Subject Item
_:vb474386532
rdf:type
n2:RelevantBibliographicResource
n2:RelevantScore
3
n2:hasRelevantPaperId
n3:19829296
Subject Item
_:vb474386533
rdf:type
n2:RelevantBibliographicResource
n2:RelevantScore
3
n2:hasRelevantPaperId
n3:14712275
Subject Item
_:vb474386534
rdf:type
n2:RelevantBibliographicResource
n2:RelevantScore
3
n2:hasRelevantPaperId
n3:19444307
Subject Item
_:vb474386535
rdf:type
n2:RelevantBibliographicResource
n2:RelevantScore
3
n2:hasRelevantPaperId
n3:19167248
Subject Item
_:vb474386536
rdf:type
n2:RelevantBibliographicResource
n2:RelevantScore
3
n2:hasRelevantPaperId
n3:20870176
Subject Item
_:vb474386537
rdf:type
n2:RelevantBibliographicResource
n2:RelevantScore
3
n2:hasRelevantPaperId
n3:17717539
Subject Item
_:vb474386538
rdf:type
n2:RelevantBibliographicResource
n2:RelevantScore
3
n2:hasRelevantPaperId
n3:18173372
Subject Item
_:vb474386539
rdf:type
n2:RelevantBibliographicResource
n2:RelevantScore
2
n2:hasRelevantPaperId
n3:18792018
Subject Item
_:vb474386540
rdf:type
n2:RelevantBibliographicResource
n2:RelevantScore
2
n2:hasRelevantPaperId
n3:20440079
Subject Item
_:vb474386541
rdf:type
n2:RelevantBibliographicResource
n2:RelevantScore
2
n2:hasRelevantPaperId
n3:22896675
Subject Item
_:vb474386542
rdf:type
n2:RelevantBibliographicResource
n2:RelevantScore
2
n2:hasRelevantPaperId
n3:23452861
Subject Item
_:vb474386543
rdf:type
n2:RelevantBibliographicResource
n2:RelevantScore
2
n2:hasRelevantPaperId
n3:26441969
Subject Item
_:vb474386544
rdf:type
n2:RelevantBibliographicResource
n2:RelevantScore
2
n2:hasRelevantPaperId
n3:30186112
Subject Item
_:vb474386545
rdf:type
n2:RelevantBibliographicResource
n2:RelevantScore
2
n2:hasRelevantPaperId
n3:15349858
Subject Item
_:vb474386546
rdf:type
n2:RelevantBibliographicResource
n2:RelevantScore
2
n2:hasRelevantPaperId
n3:20605228
Subject Item
_:vb474386547
rdf:type
n2:RelevantBibliographicResource
n2:RelevantScore
2
n2:hasRelevantPaperId
n3:23054369
Subject Item
_:vb474386548
rdf:type
n2:RelevantBibliographicResource
n2:RelevantScore
2
n2:hasRelevantPaperId
n3:23589621
Subject Item
_:vb474386549
rdf:type
n2:RelevantBibliographicResource
n2:RelevantScore
2
n2:hasRelevantPaperId
n3:29545515
Subject Item
_:vb474386550
rdf:type
n2:RelevantBibliographicResource
n2:RelevantScore
2
n2:hasRelevantPaperId
n3:9221750
Subject Item
_:vb474386551
rdf:type
n2:RelevantBibliographicResource
n2:RelevantScore
2
n2:hasRelevantPaperId
n3:12576233
Subject Item
_:vb474386552
rdf:type
n2:RelevantBibliographicResource
n2:RelevantScore
2
n2:hasRelevantPaperId
n3:21239728
Subject Item
_:vb474386553
rdf:type
n2:RelevantBibliographicResource
n2:RelevantScore
2
n2:hasRelevantPaperId
n3:26255669
Subject Item
_:vb474386554
rdf:type
n2:RelevantBibliographicResource
n2:RelevantScore
2
n2:hasRelevantPaperId
n3:26876939
Subject Item
_:vb474386555
rdf:type
n2:RelevantBibliographicResource
n2:RelevantScore
2
n2:hasRelevantPaperId
n3:27616557
Subject Item
_:vb474386556
rdf:type
n2:RelevantBibliographicResource
n2:RelevantScore
2
n2:hasRelevantPaperId
n3:7937744
Subject Item
_:vb474386557
rdf:type
n2:RelevantBibliographicResource
n2:RelevantScore
2
n2:hasRelevantPaperId
n3:23920495
Subject Item
_:vb474386558
rdf:type
n2:RelevantBibliographicResource
n2:RelevantScore
2
n2:hasRelevantPaperId
n3:25152710
Subject Item
_:vb474386559
rdf:type
n2:RelevantBibliographicResource
n2:RelevantScore
2
n2:hasRelevantPaperId
n3:12415310
Subject Item
_:vb474386560
rdf:type
n2:RelevantBibliographicResource
n2:RelevantScore
2
n2:hasRelevantPaperId
n3:18573909
Subject Item
_:vb474386561
rdf:type
n2:RelevantBibliographicResource
n2:RelevantScore
2
n2:hasRelevantPaperId
n3:20623124
Subject Item
_:vb474386562
rdf:type
n2:RelevantBibliographicResource
n2:RelevantScore
2
n2:hasRelevantPaperId
n3:22131937
Subject Item
_:vb474386563
rdf:type
n2:RelevantBibliographicResource
n2:RelevantScore
2
n2:hasRelevantPaperId
n3:23598650
Subject Item
_:vb474386564
rdf:type
n2:RelevantBibliographicResource
n2:RelevantScore
2
n2:hasRelevantPaperId
n3:26669439
Subject Item
_:vb474386565
rdf:type
n2:RelevantBibliographicResource
n2:RelevantScore
2
n2:hasRelevantPaperId
n3:30647813
Subject Item
_:vb474386566
rdf:type
n2:RelevantBibliographicResource
n2:RelevantScore
2
n2:hasRelevantPaperId
n3:14684867
Subject Item
_:vb474386567
rdf:type
n2:RelevantBibliographicResource
n2:RelevantScore
2
n2:hasRelevantPaperId
n3:22293174
Subject Item
_:vb474386568
rdf:type
n2:RelevantBibliographicResource
n2:RelevantScore
2
n2:hasRelevantPaperId
n3:29917120
Subject Item
_:vb474386569
rdf:type
n2:RelevantBibliographicResource
n2:RelevantScore
2
n2:hasRelevantPaperId
n3:21919736
Subject Item
_:vb474386570
rdf:type
n2:RelevantBibliographicResource
n2:RelevantScore
2
n2:hasRelevantPaperId
n3:24556681
Subject Item
_:vb474386571
rdf:type
n2:RelevantBibliographicResource
n2:RelevantScore
2
n2:hasRelevantPaperId
n3:15368284
Subject Item
_:vb474386572
rdf:type
n2:RelevantBibliographicResource
n2:RelevantScore
2
n2:hasRelevantPaperId
n3:20623286
Subject Item
_:vb474386573
rdf:type
n2:RelevantBibliographicResource
n2:RelevantScore
2
n2:hasRelevantPaperId
n3:15543150
Subject Item
_:vb474386574
rdf:type
n2:RelevantBibliographicResource
n2:RelevantScore
2
n2:hasRelevantPaperId
n3:16299478
Subject Item
_:vb474386575
rdf:type
n2:RelevantBibliographicResource
n2:RelevantScore
2
n2:hasRelevantPaperId
n3:18278054
Subject Item
_:vb474386576
rdf:type
n2:RelevantBibliographicResource
n2:RelevantScore
2
n2:hasRelevantPaperId
n3:19144568
Subject Item
_:vb474386577
rdf:type
n2:RelevantBibliographicResource
n2:RelevantScore
2
n2:hasRelevantPaperId
n3:22296715
Subject Item
_:vb474386578
rdf:type
n2:RelevantBibliographicResource
n2:RelevantScore
2
n2:hasRelevantPaperId
n3:28839214
Subject Item
_:vb474386579
rdf:type
n2:RelevantBibliographicResource
n2:RelevantScore
2
n2:hasRelevantPaperId
n3:19836266
Subject Item
_:vb474386580
rdf:type
n2:RelevantBibliographicResource
n2:RelevantScore
2
n2:hasRelevantPaperId
n3:15928201
Subject Item
_:vb474386581
rdf:type
n2:RelevantBibliographicResource
n2:RelevantScore
2
n2:hasRelevantPaperId
n3:18579442
Subject Item
_:vb474386582
rdf:type
n2:RelevantBibliographicResource
n2:RelevantScore
2
n2:hasRelevantPaperId
n3:20637861
Subject Item
_:vb474386583
rdf:type
n2:RelevantBibliographicResource
n2:RelevantScore
2
n2:hasRelevantPaperId
n3:11745370
Subject Item
_:vb474386584
rdf:type
n2:RelevantBibliographicResource
n2:RelevantScore
2
n2:hasRelevantPaperId
n3:17417634
Subject Item
_:vb474386585
rdf:type
n2:RelevantBibliographicResource
n2:RelevantScore
2
n2:hasRelevantPaperId
n3:20134419
Subject Item
_:vb474386586
rdf:type
n2:RelevantBibliographicResource
n2:RelevantScore
2
n2:hasRelevantPaperId
n3:26964686
Subject Item
_:vb474386587
rdf:type
n2:RelevantBibliographicResource
n2:RelevantScore
2
n2:hasRelevantPaperId
n3:12781128
Subject Item
_:vb474386588
rdf:type
n2:RelevantBibliographicResource
n2:RelevantScore
2
n2:hasRelevantPaperId
n3:17114485
Subject Item
_:vb474386589
rdf:type
n2:RelevantBibliographicResource
n2:RelevantScore
2
n2:hasRelevantPaperId
n3:17709958
Subject Item
_:vb474386590
rdf:type
n2:RelevantBibliographicResource
n2:RelevantScore
2
n2:hasRelevantPaperId
n3:27229916
Subject Item
_:vb474386591
rdf:type
n2:RelevantBibliographicResource
n2:RelevantScore
2
n2:hasRelevantPaperId
n3:8811196
Subject Item
_:vb474386592
rdf:type
n2:RelevantBibliographicResource
n2:RelevantScore
2
n2:hasRelevantPaperId
n3:15379901
Subject Item
_:vb474386593
rdf:type
n2:RelevantBibliographicResource
n2:RelevantScore
2
n2:hasRelevantPaperId
n3:29608988
Subject Item
_:vb474386594
rdf:type
n2:RelevantBibliographicResource
n2:RelevantScore
2
n2:hasRelevantPaperId
n3:10854289
Subject Item
_:vb474386595
rdf:type
n2:RelevantBibliographicResource
n2:RelevantScore
2
n2:hasRelevantPaperId
n3:12615204
Subject Item
_:vb474386596
rdf:type
n2:RelevantBibliographicResource
n2:RelevantScore
2
n2:hasRelevantPaperId
n3:23337699
Subject Item
_:vb474386597
rdf:type
n2:RelevantBibliographicResource
n2:RelevantScore
2
n2:hasRelevantPaperId
n3:24399752
Subject Item
_:vb474386598
rdf:type
n2:RelevantBibliographicResource
n2:RelevantScore
2
n2:hasRelevantPaperId
n3:27702698
Subject Item
_:vb474386599
rdf:type
n2:RelevantBibliographicResource
n2:RelevantScore
2
n2:hasRelevantPaperId
n3:12225888
Subject Item
_:vb474386600
rdf:type
n2:RelevantBibliographicResource
n2:RelevantScore
2
n2:hasRelevantPaperId
n3:23529826
Subject Item
_:vb474386601
rdf:type
n2:RelevantBibliographicResource
n2:RelevantScore
2
n2:hasRelevantPaperId
n3:15944226
Subject Item
_:vb474386602
rdf:type
n2:RelevantBibliographicResource
n2:RelevantScore
2
n2:hasRelevantPaperId
n3:22722867
Subject Item
_:vb474386603
rdf:type
n2:RelevantBibliographicResource
n2:RelevantScore
2
n2:hasRelevantPaperId
n3:16393993
Subject Item
_:vb474386604
rdf:type
n2:RelevantBibliographicResource
n2:RelevantScore
2
n2:hasRelevantPaperId
n3:16964261
Subject Item
_:vb474386605
rdf:type
n2:RelevantBibliographicResource
n2:RelevantScore
2
n2:hasRelevantPaperId
n3:18711433
Subject Item
_:vb474386606
rdf:type
n2:RelevantBibliographicResource
n2:RelevantScore
2
n2:hasRelevantPaperId
n3:30061532
Subject Item
_:vb474386607
rdf:type
n2:RelevantBibliographicResource
n2:RelevantScore
2
n2:hasRelevantPaperId
n3:30389931
Subject Item
_:vb474386608
rdf:type
n2:RelevantBibliographicResource
n2:RelevantScore
2
n2:hasRelevantPaperId
n3:14523098
Subject Item
_:vb474386609
rdf:type
n2:RelevantBibliographicResource
n2:RelevantScore
2
n2:hasRelevantPaperId
n3:27493626
Subject Item
_:vb474386610
rdf:type
n2:RelevantBibliographicResource
n2:RelevantScore
2
n2:hasRelevantPaperId
n3:10993917
Subject Item
_:vb474386611
rdf:type
n2:RelevantBibliographicResource
n2:RelevantScore
2
n2:hasRelevantPaperId
n3:18362179
Subject Item
_:vb474386612
rdf:type
n2:RelevantBibliographicResource
n2:RelevantScore
2
n2:hasRelevantPaperId
n3:19179377
Subject Item
_:vb474386613
rdf:type
n2:RelevantBibliographicResource
n2:RelevantScore
2
n2:hasRelevantPaperId
n3:22341445
Subject Item
_:vb474386614
rdf:type
n2:RelevantBibliographicResource
n2:RelevantScore
2
n2:hasRelevantPaperId
n3:23435332
Subject Item
_:vb474386615
rdf:type
n2:RelevantBibliographicResource
n2:RelevantScore
2
n2:hasRelevantPaperId
n3:25059097
Subject Item
_:vb474386616
rdf:type
n2:RelevantBibliographicResource
n2:RelevantScore
2
n2:hasRelevantPaperId
n3:27098648
Subject Item
_:vb474386617
rdf:type
n2:RelevantBibliographicResource
n2:RelevantScore
2
n2:hasRelevantPaperId
n3:29078813
Subject Item
_:vb474386618
rdf:type
n2:RelevantBibliographicResource
n2:RelevantScore
2
n2:hasRelevantPaperId
n3:8359494
Subject Item
_:vb474386619
rdf:type
n2:RelevantBibliographicResource
n2:RelevantScore
2
n2:hasRelevantPaperId
n3:12871638
Subject Item
_:vb474386620
rdf:type
n2:RelevantBibliographicResource
n2:RelevantScore
2
n2:hasRelevantPaperId
n3:15831717
Subject Item
_:vb474386621
rdf:type
n2:RelevantBibliographicResource
n2:RelevantScore
2
n2:hasRelevantPaperId
n3:18496841
Subject Item
_:vb474386622
rdf:type
n2:RelevantBibliographicResource
n2:RelevantScore
2
n2:hasRelevantPaperId
n3:25268136
Subject Item
_:vb474386623
rdf:type
n2:RelevantBibliographicResource
n2:RelevantScore
2
n2:hasRelevantPaperId
n3:12504866
Subject Item
_:vb474386624
rdf:type
n2:RelevantBibliographicResource
n2:RelevantScore
2
n2:hasRelevantPaperId
n3:16818754
Subject Item
_:vb474386625
rdf:type
n2:RelevantBibliographicResource
n2:RelevantScore
2
n2:hasRelevantPaperId
n3:20723761
Subject Item
_:vb474386626
rdf:type
n2:RelevantBibliographicResource
n2:RelevantScore
2
n2:hasRelevantPaperId
n3:21166679
Subject Item
_:vb474386627
rdf:type
n2:RelevantBibliographicResource
n2:RelevantScore
2
n2:hasRelevantPaperId
n3:24736453
Subject Item
_:vb474386628
rdf:type
n2:RelevantBibliographicResource
n2:RelevantScore
2
n2:hasRelevantPaperId
n3:29759483
Subject Item
_:vb474386629
rdf:type
n2:RelevantBibliographicResource
n2:RelevantScore
2
n2:hasRelevantPaperId
n3:12016203
Subject Item
_:vb474386630
rdf:type
n2:RelevantBibliographicResource
n2:RelevantScore
2
n2:hasRelevantPaperId
n3:20332793
Subject Item
_:vb474386631
rdf:type
n2:RelevantBibliographicResource
n2:RelevantScore
2
n2:hasRelevantPaperId
n3:26431936
Subject Item
_:vb474386632
rdf:type
n2:RelevantBibliographicResource
n2:RelevantScore
2
n2:hasRelevantPaperId
n3:27802243
Subject Item
_:vb474386633
rdf:type
n2:RelevantBibliographicResource
n2:RelevantScore
2
n2:hasRelevantPaperId
n3:8371781
Subject Item
_:vb474386634
rdf:type
n2:RelevantBibliographicResource
n2:RelevantScore
2
n2:hasRelevantPaperId
n3:12876559
Subject Item
_:vb474386635
rdf:type
n2:RelevantBibliographicResource
n2:RelevantScore
2
n2:hasRelevantPaperId
n3:18848864
Subject Item
_:vb474386636
rdf:type
n2:RelevantBibliographicResource
n2:RelevantScore
2
n2:hasRelevantPaperId
n3:19339593
Subject Item
_:vb474386637
rdf:type
n2:RelevantBibliographicResource
n2:RelevantScore
2
n2:hasRelevantPaperId
n3:20566674
Subject Item
_:vb474386638
rdf:type
n2:RelevantBibliographicResource
n2:RelevantScore
2
n2:hasRelevantPaperId
n3:29428213
Subject Item
_:vb474386639
rdf:type
n2:RelevantBibliographicResource
n2:RelevantScore
2
n2:hasRelevantPaperId
n3:14565534
Subject Item
_:vb474386640
rdf:type
n2:RelevantBibliographicResource
n2:RelevantScore
2
n2:hasRelevantPaperId
n3:29774058
Subject Item
_:vb474386641
rdf:type
n2:RelevantBibliographicResource
n2:RelevantScore
2
n2:hasRelevantPaperId
n3:23448240
Subject Item
_:vb474386642
rdf:type
n2:RelevantBibliographicResource
n2:RelevantScore
2
n2:hasRelevantPaperId
n3:30128145
Subject Item
_:vb474386643
rdf:type
n2:RelevantBibliographicResource
n2:RelevantScore
2
n2:hasRelevantPaperId
n3:23571678
Subject Item
_:vb474386644
rdf:type
n2:RelevantBibliographicResource
n2:RelevantScore
2
n2:hasRelevantPaperId
n3:28283756
Subject Item
_:vb474386645
rdf:type
n2:RelevantBibliographicResource
n2:RelevantScore
2
n2:hasRelevantPaperId
n3:2321027
Subject Item
_:vb474386646
rdf:type
n2:RelevantBibliographicResource
n2:RelevantScore
2
n2:hasRelevantPaperId
n3:14668803
Subject Item
_:vb474386647
rdf:type
n2:RelevantBibliographicResource
n2:RelevantScore
2
n2:hasRelevantPaperId
n3:20812720
Subject Item
_:vb474386648
rdf:type
n2:RelevantBibliographicResource
n2:RelevantScore
2
n2:hasRelevantPaperId
n3:23219269
Subject Item
_:vb474386649
rdf:type
n2:RelevantBibliographicResource
n2:RelevantScore
2
n2:hasRelevantPaperId
n3:15895084
Subject Item
_:vb474386650
rdf:type
n2:RelevantBibliographicResource
n2:RelevantScore
2
n2:hasRelevantPaperId
n3:19896530
Subject Item
_:vb474386651
rdf:type
n2:RelevantBibliographicResource
n2:RelevantScore
2
n2:hasRelevantPaperId
n3:25352786
Subject Item
_:vb474386652
rdf:type
n2:RelevantBibliographicResource
n2:RelevantScore
2
n2:hasRelevantPaperId
n3:26101267
Subject Item
_:vb474386653
rdf:type
n2:RelevantBibliographicResource
n2:RelevantScore
2
n2:hasRelevantPaperId
n3:10760787
Subject Item
_:vb474386654
rdf:type
n2:RelevantBibliographicResource
n2:RelevantScore
2
n2:hasRelevantPaperId
n3:21235577
Subject Item
_:vb474386655
rdf:type
n2:RelevantBibliographicResource
n2:RelevantScore
2
n2:hasRelevantPaperId
n3:22727420
Subject Item
_:vb474386656
rdf:type
n2:RelevantBibliographicResource
n2:RelevantScore
2
n2:hasRelevantPaperId
n3:15735651