_:b546166821 . _:b546166652 . _:b546166717 . _:b546166737 "2"^^ . _:b546166797 . _:b546166820 . . _:b546166736 "2"^^ . _:b546166716 . _:b546166823 . . _:b546166739 "2"^^ . _:b546166719 . _:b546166517 . _:b546166364 . _:b546166822 . . . _:b546166718 . _:b546166738 "2"^^ . _:b546166817 . _:b546166863 . _:b546166713 . _:b546166749 "2"^^ . _:b29203201 "AQP5 (aquaporin5) is present on the apical membrane of ductal cells in virgin glands, and its expression is normally turned off at early pregnancy (Shillingford et al. >>2003<<). NKCC1 is a Na\u2013K\u2013Cl cotransporter that is enriched on the basolateral membrane of ductal mammary epithelial cells in virgin glands, and its expression is normally turned off in developing alveoli and ductal structures at mid pregnancy" . _:b546166816 . _:b546166637 . _:b546166712 . _:b546166748 "2"^^ . . _:b546166819 . _:b546166715 . _:b546166751 "2"^^ . _:b546166740 . _:b546166818 . . _:b546166750 "2"^^ . _:b546166714 . _:b546166829 . _:b546166658 . _:b546166725 . _:b546166745 "2"^^ . _:b546166600 . _:b546166828 . _:b546166724 . _:b546166744 "2"^^ . _:b546166758 . _:b546166831 . _:b546166523 . _:b546166747 "2"^^ . _:b546166727 . . _:b546166830 . . . _:b546166448 . _:b546166746 "2"^^ . _:b546166726 . _:b546166449 . _:b546166450 . _:b546166825 . . _:b546166451 . _:b546166425 . _:b546166452 . _:b546166757 "2"^^ . _:b546166721 . _:b546166453 . _:b546166454 . _:b546166824 . _:b546166455 . _:b546166456 . _:b546166756 "2"^^ . _:b546166720 . _:b546166457 . _:b546166458 . _:b546166827 . . _:b546166459 . _:b546166460 . _:b546166759 "2"^^ . _:b546166723 . _:b546166461 . _:b546166800 . _:b546166462 . _:b546166826 . _:b546166463 . . _:b546166432 . _:b546166758 "2"^^ . _:b546166722 . . _:b546166433 . . _:b546166434 . _:b546166837 . _:b546166435 . _:b546166436 . _:b546166753 "2"^^ . _:b546166733 . . _:b546166839 . _:b546166437 . _:b546166438 . _:b546166836 . _:b546166439 . _:b546166440 . _:b546166752 "2"^^ . _:b546166732 . _:b546166441 . _:b546166442 . _:b546166839 . _:b546166443 . . _:b546166444 . _:b546166755 "2"^^ . _:b546166735 . _:b546166445 . _:b546166522 . _:b546166446 . _:b546166838 . _:b546166447 . _:b546166416 . _:b546166754 "2"^^ . _:b546166734 . _:b546166417 . _:b546166418 . _:b546166833 . _:b546166725 . _:b546166344 . _:b29203195 "loss-of-function or gain-of-function of YAP by crossing floxed alleles of Yap or Sav1 (encoding a well-established tumor suppressor and negative regulator of the YAP oncoprotein) with the MMTV-Cre recombinase (Wagner et al. >>1997<<). Successful deletion of Yap or Sav1 was confirmed, and the expression of the Yap paralog Taz was unchanged in the Yap-deficient mammary gland (Supplemental Fig." . . _:b546166419 . _:b546166420 . _:b546166765 "2"^^ . _:b546166729 . _:b546166575 . _:b546166421 . _:b546166422 . _:b546166832 . . _:b546166423 . _:b546166424 . _:b546166764 "2"^^ . _:b546166728 . _:b546166425 . _:b546166426 . _:b546166876 . _:b546166835 . _:b546166427 . _:b546166726 . _:b546166428 . _:b546166767 "2"^^ . _:b546166731 . _:b546166429 . _:b546166430 . _:b546166834 . _:b546166431 . _:b546166400 . _:b546166766 "2"^^ . _:b546166730 . . _:b546166401 . _:b546166402 . _:b546166845 . . _:b546166403 . _:b546166404 . _:b546166761 "2"^^ . _:b546166741 . _:b546166405 . _:b546166343 . _:b546166406 . _:b546166844 . . _:b546166775 . _:b546166715 . _:b546166407 . _:b546166408 . _:b546166760 "2"^^ . _:b546166740 . _:b546166409 . _:b546166410 . _:b546166847 . _:b546166489 . _:b546166411 . _:b546166412 . _:b546166763 "2"^^ . _:b546166743 . _:b546166413 . _:b546166414 . _:b546166846 . . _:b546166415 . _:b546166512 . _:b546166762 "2"^^ . _:b546166742 . _:b546166513 . _:b546166514 . _:b29203200 "S2F; Guelstein et al. 1988; Wetzels et al. >>1989<<). To further evaluate the differentiation defects, we examined two markers that are normally expressed in virgin mammary glands but are down-regulated at earlier stages of pregnancy." . _:b546166841 . . _:b546166515 . . _:b546166773 "2"^^ . _:b546166737 . _:b546166516 . _:b546166517 . . _:b546166840 . _:b546166552 . _:b546166518 . _:b546166519 . . _:b546166772 "2"^^ . _:b546166736 . _:b546166520 . _:b546166521 . _:b546166522 . _:b546166843 . _:b546166481 . _:b546166523 . . _:b546166838 . _:b546166775 "2"^^ . _:b546166739 . _:b546166524 . _:b546166525 . _:b546166526 . _:b546166842 . _:b546166527 . _:b546166496 . _:b546166774 "2"^^ . _:b546166738 . _:b546166497 . _:b546166498 . _:b546166853 . _:b546166580 . . _:b546166812 . _:b546166499 . . _:b546166500 . _:b546166769 "2"^^ . _:b546166749 . . _:b546166501 . _:b546166502 . _:b546166852 . _:b546166503 . _:b546166504 . _:b546166768 "2"^^ . _:b546166748 . . . _:b546166378 . _:b546166505 . . _:b546166506 . _:b546166855 . _:b546166507 . _:b546166508 . _:b546166771 "2"^^ . _:b546166751 . _:b546166427 . . _:b546166509 . . _:b546166854 . _:b546166510 . _:b546166511 . . _:b546166770 "2"^^ . _:b546166750 . _:b546166480 . _:b546166481 . _:b546166482 . _:b546166849 . _:b546166483 . . _:b546166781 "2"^^ . _:b546166745 . _:b546166484 . _:b546166485 . _:b546166486 . _:b546166848 . . _:b546166487 . _:b546166488 . _:b546166780 "2"^^ . _:b546166744 . _:b546166489 . _:b546166830 . . _:b546166851 . _:b546166490 . _:b546166491 . . _:b546166783 "2"^^ . _:b546166747 . _:b546166492 . . _:b546166493 . _:b546166494 . _:b546166850 . . . _:b546166495 . _:b546166464 . _:b546166782 "2"^^ . _:b546166746 . . _:b546166465 . _:b546166466 . _:b546166861 . _:b546166467 . _:b546166468 . _:b546166777 "2"^^ . _:b546166757 . _:b546166469 . _:b546166470 . _:b546166860 . . _:b546166471 . _:b546166472 . _:b546166776 "2"^^ . _:b546166756 . _:b546166473 . _:b546166546 . _:b546166474 . _:b546166863 . . _:b546166687 . _:b546166475 . _:b546166476 . _:b546166779 "2"^^ . _:b546166759 . . _:b546166477 . _:b546166843 . _:b546166789 "2"^^ . _:b546166509 . _:b546166399 . _:b546166862 . _:b546166478 . _:b546166479 . . _:b546166778 "2"^^ . _:b546166758 . _:b546166705 . . _:b546166357 . _:b546166788 "2"^^ . . _:b546166857 . _:b546166753 . . _:b546166791 "2"^^ . _:b546166856 . . _:b546166667 . _:b546166444 . _:b546166752 . _:b546166790 "2"^^ . . _:b546166859 . . . _:b546166755 . _:b546166785 "2"^^ . . _:b546166858 . . _:b546166754 . . _:b546166784 "2"^^ . . _:b546166869 . _:b546166765 . _:b546166787 "2"^^ . . _:b546166868 . _:b546166764 . _:b546166786 "2"^^ . _:b546166827 . _:b546166871 . . _:b546166767 . . _:b546166797 "2"^^ . _:b546166397 . _:b546166870 . _:b546166766 . _:b546166796 "2"^^ . . _:b546166865 . _:b546166761 . . _:b546166799 "2"^^ . _:b546166498 . _:b546166864 . _:b546166688 . _:b546166760 . _:b546166798 "2"^^ . _:b546166867 . _:b546166763 . . _:b546166793 "2"^^ . _:b546166866 . _:b546166762 . _:b546166776 . _:b546166792 "2"^^ . _:b546166877 . . _:b546166773 . _:b546166795 "2"^^ . . _:b546166876 . _:b546166852 . _:b546166772 . _:b546166794 "2"^^ . _:b546166762 . _:b546166879 . _:b546166775 . . _:b546166805 "2"^^ . _:b546166873 . _:b546166878 . _:b546166709 . _:b546166384 . _:b546166774 . . _:b546166804 "2"^^ . _:b546166385 . . _:b546166375 . . _:b546166873 . _:b546166386 . . _:b546166387 . . _:b546166769 . _:b546166388 . _:b546166389 . _:b546166807 "2"^^ . _:b546166390 . _:b546166872 . _:b546166391 . . _:b546166768 . _:b546166748 . _:b546166392 . _:b546166393 . _:b546166806 "2"^^ . . _:b546166875 . _:b546166394 . . _:b546166450 . _:b546166395 . _:b546166396 . _:b546166771 . _:b546166577 . _:b546166397 . _:b546166801 "2"^^ . . _:b546166874 . _:b546166506 . _:b546166398 . . _:b546166399 . _:b546166368 . _:b546166770 . _:b546166369 . _:b546166800 "2"^^ . _:b546166370 . _:b546166371 . _:b546166372 . _:b546166781 . _:b546166373 . _:b546166803 "2"^^ . _:b546166374 . _:b546166375 . . _:b546166780 . _:b546166376 . _:b546166377 . _:b546166802 "2"^^ . _:b546166378 . _:b546166379 . _:b546166380 . _:b546166783 . _:b546166381 . _:b546166813 "2"^^ . _:b546166382 . _:b546166383 . _:b546166352 . _:b546166782 . _:b546166353 . _:b546166812 "2"^^ . _:b546166354 . _:b546166355 . _:b546166356 . _:b546166777 . _:b546166756 . _:b546166357 . _:b546166815 "2"^^ . _:b546166358 . _:b546166880 . _:b546166359 . _:b546166360 . _:b546166776 . _:b546166361 . _:b546166814 "2"^^ . _:b546166362 . _:b546166363 . _:b546166364 . _:b546166779 . _:b546166365 . _:b546166809 "2"^^ . _:b546166366 . . _:b546166367 . . _:b546166778 . _:b546166808 "2"^^ . . _:b546166856 . _:b546166341 . _:b546166811 "2"^^ . _:b546166342 . _:b546166343 . _:b546166794 . . _:b546166344 . . _:b546166810 "2"^^ . _:b546166345 . _:b546166346 . _:b546166347 . _:b546166348 . _:b546166349 . _:b546166821 "2"^^ . . _:b546166350 . _:b546166351 . _:b546166704 . _:b546166746 . _:b546166705 . _:b546166820 "2"^^ . _:b546166538 . _:b546166706 . _:b546166707 . _:b546166528 . _:b546166708 . _:b546166709 . _:b546166823 "2"^^ . _:b546166710 . _:b546166475 . _:b546166711 . _:b546166712 . . _:b546166822 "2"^^ . _:b546166713 . _:b546166714 . _:b546166715 . _:b546166716 . _:b29203196 . _:b546166458 . _:b546166717 . _:b546166817 "2"^^ . _:b546166718 . _:b546166628 . _:b546166719 . _:b546166688 . _:b546166689 . _:b546166816 "2"^^ . _:b546166615 . _:b546166543 . _:b546166413 . _:b546166690 . _:b546166691 . _:b546166692 . _:b546166556 . _:b546166693 . _:b546166819 "2"^^ . _:b546166579 . _:b546166694 . _:b546166695 . . _:b546166696 . . _:b546166697 . _:b546166818 "2"^^ . _:b546166698 . _:b546166699 . _:b546166700 . _:b546166701 . _:b546166829 "2"^^ . _:b546166650 . _:b546166702 . _:b546166703 . _:b546166672 . _:b546166731 . _:b546166673 . _:b546166828 "2"^^ . _:b546166674 . _:b546166675 . . _:b546166676 . _:b546166677 . _:b546166831 "2"^^ . _:b546166678 . _:b546166824 . _:b546166679 . _:b546166680 . _:b546166681 . _:b546166830 "2"^^ . _:b546166550 . _:b546166682 . _:b546166683 . . _:b546166684 . . _:b546166685 . _:b546166825 "2"^^ . _:b546166686 . _:b546166687 . . _:b546166656 . . _:b546166657 . _:b546166824 "2"^^ . _:b546166658 . _:b546166659 . _:b546166570 . . _:b546166430 . _:b546166660 . _:b546166661 . _:b546166827 "2"^^ . _:b546166662 . _:b546166663 . _:b546166664 . _:b546166665 . _:b546166826 "2"^^ . _:b546166666 . _:b546166717 . _:b546166667 . _:b546166668 . _:b546166728 . _:b546166669 . _:b546166837 "2"^^ . _:b546166670 . _:b546166844 . _:b546166534 . _:b546166671 . . _:b546166768 . . _:b546166836 "2"^^ . _:b546166769 . _:b546166770 . _:b546166771 . _:b546166529 . _:b546166772 . _:b546166773 . _:b546166839 "2"^^ . _:b546166774 . _:b546166775 . _:b546166776 . _:b546166590 . _:b546166777 . _:b546166838 "2"^^ . _:b546166778 . _:b546166779 . _:b546166780 . _:b546166781 . _:b546166833 "2"^^ . _:b546166782 . . _:b546166783 . _:b546166752 . _:b546166783 . . _:b546166832 "2"^^ . _:b546166662 . _:b546166753 . _:b546166754 . . _:b546166755 . _:b546166756 . _:b546166757 . _:b546166835 "2"^^ . _:b546166758 . _:b546166759 . _:b546166760 . _:b546166761 . _:b546166834 "2"^^ . _:b546166762 . _:b546166763 . _:b546166376 . _:b546166764 . _:b546166765 . _:b546166845 "2"^^ . _:b546166766 . . _:b546166767 . . _:b546166777 . _:b546166736 . _:b546166737 . _:b546166844 "2"^^ . _:b546166738 . _:b546166739 . _:b546166740 . _:b546166741 . _:b546166847 "2"^^ . _:b546166742 . _:b546166743 . _:b546166744 . _:b546166745 . _:b29203204 . _:b546166846 "2"^^ . _:b546166746 . _:b546166747 . _:b546166748 . _:b546166749 . _:b29203205 . _:b546166841 "2"^^ . _:b546166750 . _:b546166751 . . _:b546166720 . _:b546166721 . _:b29203206 . _:b546166840 "2"^^ . _:b546166722 . _:b546166723 . _:b546166724 . _:b546166801 . _:b546166725 . _:b29203207 . _:b546166843 "2"^^ . _:b546166778 . _:b546166726 . _:b546166727 . _:b546166728 . _:b546166729 . _:b29203200 . _:b546166842 "2"^^ . . . _:b546166374 . _:b546166730 . _:b546166731 . _:b546166732 . . _:b546166733 . _:b29203201 . _:b546166853 "2"^^ . _:b546166734 . . _:b546166735 . _:b546166576 . _:b546166660 . _:b546166577 . _:b29203208 . _:b29203202 . _:b546166852 "2"^^ . _:b546166578 . _:b546166579 . _:b546166580 . _:b546166581 . _:b29203203 . _:b546166855 "2"^^ . _:b546166582 . _:b546166583 . _:b546166584 . _:b546166585 . _:b546166854 "2"^^ . _:b546166586 . _:b546166587 . _:b546166588 . _:b546166589 . _:b546166849 "2"^^ . _:b546166840 . _:b546166590 . _:b546166353 . _:b546166591 . _:b546166560 . _:b546166382 . _:b546166561 . _:b546166848 "2"^^ . _:b546166562 . _:b546166563 . . . _:b546166564 . _:b546166565 . _:b546166851 "2"^^ . _:b546166566 . _:b546166682 . _:b546166567 . _:b546166436 . _:b546166568 . _:b546166569 . _:b29203208 . _:b546166850 "2"^^ . _:b546166570 . _:b546166571 . . _:b546166572 . _:b546166573 . _:b546166861 "2"^^ . _:b546166574 . _:b546166771 . . _:b546166421 . _:b546166575 . _:b546166544 . _:b546166545 . _:b546166860 "2"^^ . . _:b546166546 . _:b546166723 . . _:b546166547 . _:b546166548 . _:b546166549 . _:b546166863 "2"^^ . _:b546166550 . _:b546166694 . . _:b546166551 . _:b546166552 . _:b546166553 . _:b546166862 "2"^^ . _:b546166554 . _:b546166555 . . _:b546166556 . _:b546166557 . _:b546166857 "2"^^ . _:b546166558 . . _:b546166559 . _:b546166528 . . _:b546166529 . _:b546166856 "2"^^ . _:b546166530 . _:b546166531 . . _:b546166532 . . _:b546166533 . _:b546166859 "2"^^ . _:b546166534 . _:b546166872 . _:b546166535 . _:b546166536 . _:b546166721 . _:b546166537 . _:b546166858 "2"^^ . _:b546166631 . _:b546166538 . _:b546166539 . _:b546166569 . _:b546166540 . _:b546166541 . _:b546166869 "2"^^ . _:b546166542 . _:b546166578 . _:b546166543 . _:b546166640 . _:b546166641 . _:b546166868 "2"^^ . _:b546166642 . _:b546166643 . _:b546166644 . _:b546166796 . _:b546166645 . _:b546166871 "2"^^ . _:b546166646 . _:b546166647 . . _:b546166648 . . _:b546166649 . _:b546166870 "2"^^ . _:b546166650 . _:b546166651 . _:b546166591 . _:b546166652 . _:b546166653 . _:b546166865 "2"^^ . _:b546166654 . _:b546166655 . _:b546166624 . _:b546166720 . _:b546166625 . _:b546166864 "2"^^ . _:b546166626 . _:b546166627 . _:b546166457 . _:b546166628 . . _:b546166629 . _:b546166867 "2"^^ . _:b546166630 . _:b546166631 . _:b546166632 . . _:b546166633 . _:b546166866 "2"^^ . _:b546166520 . _:b546166634 . _:b546166635 . _:b546166636 . _:b546166637 . _:b546166877 "2"^^ . _:b546166432 . _:b546166638 . _:b546166639 . _:b546166608 . _:b546166747 . _:b546166609 . _:b546166876 "2"^^ . _:b546166613 . _:b546166610 . . _:b546166611 . _:b546166612 . _:b546166647 . _:b546166613 . _:b546166879 "2"^^ . _:b546166614 . _:b546166497 . . . _:b546166615 . . . _:b546166616 . _:b546166557 . _:b546166617 . _:b546166878 "2"^^ . . _:b546166618 . _:b546166619 . _:b546166451 . _:b546166620 . _:b546166818 . _:b546166621 . _:b546166873 "2"^^ . _:b546166847 . _:b546166390 . _:b546166622 . _:b546166623 . . _:b546166592 . _:b546166593 . _:b546166872 "2"^^ . _:b546166594 . _:b546166595 . _:b546166341 . . _:b546166596 . _:b546166597 . _:b546166875 "2"^^ . . _:b546166598 . _:b546166599 . _:b546166600 . . _:b546166601 . _:b546166874 "2"^^ . . _:b546166602 . _:b546166603 . _:b546166343 . _:b546166604 . _:b546166605 . _:b546166606 . _:b546166607 . _:b546166342 . _:b546166486 . _:b546166524 . . _:b29203184 "materials and methods" . . . . _:b546166419 . . _:b29203203 . _:b546166880 "2"^^ . _:b546166349 . . _:b546166348 . _:b546166351 . . _:b546166599 . _:b546166350 . _:b546166789 . _:b546166345 . . _:b29203204 . _:b29203196 "2A,B; Liu et al. >>1996<<; Long et al. 2003; Shillingford et al. 2003), suggesting that YAP is required for cell survival but not terminal differentiation of mammary alveoli." . . _:b546166460 . _:b546166344 . _:b546166526 . _:b546166347 . . . . . _:b546166346 . _:b546166357 . _:b546166356 . _:b546166359 . . _:b29203192 . . . _:b546166358 . _:b546166353 . . . _:b546166352 . _:b29203185 . _:b546166855 . . . _:b546166422 . _:b546166355 . . . _:b546166561 . _:b546166354 . _:b546166862 . _:b546166483 . _:b546166365 . _:b546166870 . _:b546166364 . _:b546166638 . _:b546166540 . _:b546166367 . . _:b546166366 . _:b546166366 . _:b546166537 . . _:b546166361 . _:b546166360 . . _:b546166363 . "10.1101%2Fgad.233676.113" . . _:b546166359 . _:b546166362 . . _:b546166562 . _:b546166555 . . . _:b546166373 . . _:b546166632 . _:b546166621 . _:b546166372 . . _:b546166375 . . . . _:b546166813 . . _:b546166374 . _:b546166369 . _:b546166706 . _:b546166513 . _:b546166857 . _:b546166814 . _:b546166622 . _:b546166368 . . . . _:b546166371 . _:b546166525 . _:b546166370 . _:b29203203 "We tested this hypothesis through transgenic overexpression of human YAP under the control of a tetracycline response element (TRE) (Dong et al. >>2007<<). Crossing the TRE-YAP strain with the mammary-specific MMTV-rtTA driver (Gunther et al. 2002) allowed mammary-specific YAP overexpression in a doxycycline-regulated manner (Supplemental Fig. S5A). Interestingly, upon doxycycline feeding" . _:b546166381 . _:b546166547 . _:b546166380 . . . . _:b546166383 . _:b546166603 . . . . _:b546166382 . . _:b546166680 . _:b546166377 . . . _:b546166376 . _:b546166379 . _:b29203208 "Of note, a recent study showed that YAP is dispensable for intestinal development but required in regenerating intestinal epithelia, which exhibit dynamic changes of YAP protein levels (Cai et al. >>2010<<). Thus, Hippo signaling may be particularly important under conditions of rapid tissue growth and morphogenesis." . . . _:b546166673 . _:b546166447 . _:b546166732 . _:b546166378 . . . _:b546166435 . _:b546166772 . . _:b546166389 . . _:b546166477 . _:b546166388 . _:b546166760 . _:b546166391 . _:b29203188 "Yapflox and Sav1flox mice have been published (Cai et al. 2010; Zhang et al. >>2010<<). Mammary gland-specific knockout mice were generated by breeding Yapflox or Sav1flox mice with MMTV-Cre (line F) mice (Wagner et al. 1997) obtained from the National Cancer Institute Mouse Repository." . . _:b546166390 . _:b29203194 . _:b546166385 . _:b546166384 . _:b546166387 . . . . _:b546166386 . . _:b546166397 . _:b546166828 . . _:b546166396 . _:b546166646 . _:b546166399 . _:b546166398 . _:b546166503 . _:b546166346 . . _:b546166393 . . _:b546166770 . _:b546166392 . _:b546166384 . . . _:b546166864 . _:b546166395 . . _:b546166786 . . _:b546166394 . _:b546166849 . _:b546166405 . _:b29203187 "Yapflox and Sav1flox mice have been published (Cai et al. >>2010<<; Zhang et al. 2010). Mammary gland-specific knockout mice were generated by breeding Yapflox or Sav1flox mice with MMTV-Cre (line F) mice (Wagner et al. 1997) obtained from the National Cancer Institute Mouse Repository." . _:b546166493 . _:b546166404 . _:b546166407 . . _:b546166406 . . _:b546166401 . . _:b546166484 . _:b546166400 . _:b546166403 . . _:b546166402 . _:b29203197 . _:b546166542 . _:b546166413 . . _:b546166363 . _:b546166412 . . _:b546166415 . _:b546166683 . . . . _:b546166414 . . _:b546166409 . _:b29203190 . _:b546166394 . _:b546166408 . . _:b29203202 "that is enriched on the basolateral membrane of ductal mammary epithelial cells in virgin glands, and its expression is normally turned off in developing alveoli and ductal structures at mid pregnancy (Shillingford et al. >>2003<<). In Sav1-deficient mammary glands, AQP5 and NKCC1 were down-regulated by mid-pregnancy as in control glands (Fig." . _:b546166411 . . . _:b546166410 . _:b546166421 . . _:b546166420 . _:b546166343 "20"^^ . _:b546166341 "23"^^ . _:b546166423 . . _:b546166342 "21"^^ . _:b29203207 . . _:b546166479 . . _:b546166422 . _:b546166345 "19"^^ . _:b546166478 . . _:b29203185 "Pronuclear injection of linearized pTRE2-YAP transgene (Dong et al. >>2007<<) was carried out by the Johns Hopkins Transgenic Core Facility." . _:b29203192 "including ductal elongation in the virgin gland; proliferation, differentiation, and the formation of lobuloalveolar structures during pregnancy; and massive epithelial cell death in post-lactation-induced involution (Richert et al. >>2000<<; Brisken and Rajaram 2006; Watson and Khaled 2008). We first examined the temporal and spatial patterns of YAP expression by immunohistochemistry." . _:b546166417 . _:b546166344 "20"^^ . . _:b546166790 . _:b546166512 . _:b546166416 . _:b546166346 "18"^^ . _:b546166419 . . _:b546166347 "16"^^ . _:b546166387 . _:b546166464 . _:b546166418 . _:b546166586 . _:b546166348 "16"^^ . _:b546166429 . _:b546166349 "16"^^ . _:b546166367 . _:b546166350 "15"^^ . _:b546166355 . _:b546166428 . _:b546166351 "15"^^ . _:b546166846 . _:b546166431 . . _:b546166352 "14"^^ . _:b546166430 . . _:b546166359 "12"^^ . . . _:b546166353 "14"^^ . _:b546166425 . . _:b546166606 . _:b546166358 "13"^^ . _:b546166354 "14"^^ . _:b546166424 . . _:b546166357 "13"^^ . _:b546166874 . _:b546166355 "14"^^ . _:b546166427 . . _:b546166854 . _:b546166356 "14"^^ . . _:b546166426 . _:b546166502 . _:b546166681 . . _:b29203191 "results and discussion" . _:b546166437 . _:b546166519 . _:b546166859 . _:b546166665 . _:b546166597 . _:b546166436 . _:b546166439 . _:b546166367 "10"^^ . . . _:b546166438 . _:b546166366 "10"^^ . . _:b546166433 . _:b546166365 "10"^^ . _:b546166432 . . _:b546166364 "11"^^ . _:b546166435 . _:b546166363 "11"^^ . _:b546166819 . _:b546166434 . . _:b546166514 . _:b546166362 "11"^^ . _:b546166445 . _:b546166444 . _:b546166845 . _:b546166361 "12"^^ . _:b546166400 . _:b546166447 . _:b546166360 "12"^^ . . . _:b546166541 . _:b546166446 . _:b546166651 . _:b546166374 "8"^^ . . _:b546166441 . _:b546166375 "8"^^ . _:b546166440 . _:b546166505 . . _:b546166372 "9"^^ . . _:b29203188 . _:b546166443 . _:b546166641 . _:b546166373 "9"^^ . . _:b546166442 . _:b546166799 . _:b546166370 "9"^^ . _:b546166453 . _:b546166656 . _:b546166787 . _:b546166371 "9"^^ . . _:b546166452 . _:b546166368 "9"^^ . _:b546166548 . _:b546166356 . _:b546166415 . _:b546166455 . _:b546166369 "9"^^ . _:b546166454 . _:b546166382 "8"^^ . _:b546166441 . . _:b546166698 . . _:b546166449 . _:b546166383 "8"^^ . _:b546166670 . . _:b546166448 . _:b546166380 "8"^^ . . _:b546166451 . _:b546166381 "8"^^ . _:b546166809 . _:b546166450 . _:b546166378 "8"^^ . . _:b546166461 . _:b546166379 "8"^^ . _:b546166460 . _:b546166376 "8"^^ . . _:b546166463 . _:b546166377 "8"^^ . . _:b546166795 . _:b546166390 "7"^^ . _:b546166462 . . _:b546166485 . _:b546166391 "7"^^ . _:b546166457 . . _:b546166388 "7"^^ . _:b546166456 . . _:b546166443 . _:b546166389 "7"^^ . _:b546166677 . _:b546166459 . . _:b546166386 "7"^^ . _:b546166458 . _:b546166495 . _:b546166810 . _:b546166692 . . _:b546166387 "7"^^ . _:b546166469 . . _:b546166684 . . . _:b29203187 . _:b546166468 . _:b546166384 "8"^^ . _:b546166703 . _:b546166393 . _:b546166385 "7"^^ . _:b546166527 . _:b546166471 . _:b546166398 "7"^^ . _:b546166470 . _:b546166766 . _:b546166399 "7"^^ . _:b546166465 . _:b546166354 . _:b546166396 "7"^^ . _:b546166707 . _:b546166464 . . _:b546166349 . _:b546166751 . _:b546166784 . _:b546166397 "7"^^ . _:b546166516 . _:b546166467 . _:b546166394 "7"^^ . _:b546166710 . _:b546166466 . _:b546166831 . . _:b546166477 . _:b546166395 "7"^^ . _:b546166392 "7"^^ . _:b546166476 . _:b546166618 . _:b546166811 . _:b546166393 "7"^^ . _:b546166479 . . _:b546166406 "6"^^ . _:b546166456 . . . _:b546166478 . _:b546166693 . _:b546166407 "6"^^ . _:b546166423 . _:b546166473 . _:b546166404 "6"^^ . _:b546166472 . _:b546166405 "6"^^ . . _:b546166475 . _:b546166402 "6"^^ . _:b546166602 . . _:b546166474 . _:b546166403 "6"^^ . _:b546166565 . _:b546166485 . . _:b546166832 . _:b546166589 . _:b546166400 "7"^^ . _:b546166484 . _:b546166816 . _:b546166401 "7"^^ . _:b546166487 . . _:b546166744 . _:b546166685 . _:b546166414 "6"^^ . _:b546166486 . _:b546166415 "6"^^ . _:b546166481 . _:b546166381 . _:b546166412 "6"^^ . . _:b546166480 . _:b546166413 "6"^^ . . _:b546166483 . . _:b546166468 . _:b546166467 . _:b546166410 "6"^^ . _:b546166482 . . _:b546166411 "6"^^ . . _:b546166493 . _:b546166408 "6"^^ . _:b546166492 . . _:b546166452 . . _:b546166409 "6"^^ . _:b546166495 . _:b546166410 . _:b546166422 "6"^^ . _:b546166494 . . . _:b546166423 "6"^^ . _:b546166489 . _:b546166780 . _:b546166487 . _:b546166420 "6"^^ . _:b546166488 . _:b546166421 "6"^^ . _:b546166829 . _:b546166491 . _:b546166798 . _:b546166418 "6"^^ . _:b546166490 . . . _:b29203193 . _:b546166419 "6"^^ . . _:b546166501 . . _:b546166416 "6"^^ . _:b546166500 . _:b546166417 "6"^^ . _:b546166503 . . _:b546166502 . . _:b546166429 "5"^^ . _:b546166497 . . . _:b546166428 "5"^^ . _:b546166781 . _:b546166496 . . . _:b546166431 "5"^^ . _:b546166499 . _:b546166564 . . . . _:b546166430 "5"^^ . _:b546166498 . _:b546166425 "5"^^ . _:b546166509 . _:b546166733 . _:b546166424 "6"^^ . _:b546166508 . _:b546166428 . _:b546166427 "5"^^ . . _:b546166511 . _:b29203184 . _:b546166426 "5"^^ . _:b546166773 . _:b546166510 . . _:b546166437 "5"^^ . _:b29203191 . _:b546166505 . _:b546166414 . _:b546166436 "5"^^ . . _:b546166504 . _:b546166352 . _:b546166439 "5"^^ . _:b546166370 . _:b546166507 . . . _:b546166438 "5"^^ . _:b546166506 . _:b29203199 "S2F; Guelstein et al. >>1988<<; Wetzels et al. 1989). To further evaluate the differentiation defects, we examined two markers that are normally expressed in virgin mammary glands but are down-regulated at earlier stages of pregnancy." . . _:b546166433 "5"^^ . . _:b546166865 . _:b546166517 . . _:b546166730 . _:b546166432 "5"^^ . _:b546166785 . . _:b546166668 . _:b546166516 . _:b546166738 . _:b546166880 . _:b546166435 "5"^^ . _:b546166678 . _:b546166519 . _:b546166434 "5"^^ . . _:b546166518 . _:b546166445 "5"^^ . . _:b546166513 . . _:b546166444 "5"^^ . . _:b546166512 . _:b546166412 . _:b546166447 "5"^^ . _:b546166474 . _:b546166515 . _:b546166411 . _:b546166792 . . _:b546166446 "5"^^ . _:b29203205 . _:b546166514 . _:b546166441 "5"^^ . _:b546166571 . . _:b546166525 . . _:b546166440 "5"^^ . _:b546166524 . _:b546166443 "5"^^ . _:b546166585 . . _:b546166527 . _:b546166442 "5"^^ . _:b546166433 . _:b546166821 . _:b546166526 . . _:b546166453 "5"^^ . _:b546166521 . _:b29203189 "Mammary gland-specific knockout mice were generated by breeding Yapflox or Sav1flox mice with MMTV-Cre (line F) mice (Wagner et al. >>1997<<) obtained from the National Cancer Institute Mouse Repository." . _:b546166866 . _:b546166452 "5"^^ . _:b546166841 . . _:b546166520 . . . _:b546166455 "5"^^ . _:b546166523 . _:b546166405 . _:b546166454 "5"^^ . _:b546166536 . _:b546166522 . . _:b546166449 "5"^^ . _:b546166802 . _:b546166471 . _:b546166627 . _:b546166448 "5"^^ . _:b546166451 "5"^^ . _:b29203191 _:b29203208 . _:b546166697 . _:b546166450 "5"^^ . _:b546166878 . _:b546166768 . _:b546166455 . _:b546166601 . _:b546166510 . _:b546166461 "4"^^ . _:b29203191 _:b29203200 . _:b29203191 _:b29203201 . _:b546166460 "4"^^ . _:b29203191 _:b29203202 . _:b29203191 _:b29203203 . _:b29203191 _:b29203204 . _:b29203191 _:b29203205 . _:b546166463 "4"^^ . . . _:b29203191 _:b29203206 . _:b29203191 _:b29203207 . _:b546166462 "4"^^ . _:b546166469 . _:b546166463 . . _:b546166644 . _:b546166457 "4"^^ . . _:b546166462 . _:b546166456 "4"^^ . . . . . _:b546166459 "4"^^ . . _:b546166358 . _:b546166458 "4"^^ . _:b546166701 . . _:b546166469 "4"^^ . _:b546166654 . _:b546166434 . _:b546166468 "4"^^ . _:b546166655 . . _:b546166471 "4"^^ . . . _:b29203201 . _:b546166470 "4"^^ . _:b546166664 . _:b546166465 "4"^^ . . _:b29203206 "purpose, we took advantage of verteporfin (VP), a recently reported YAP inhibitor that interferes with the physical association between YAP and its obligatory DNA-binding transcription factor partner, TEAD/TEF (Liu-Chittenden et al. >>2012<<). A collection of eight commonly used human breast cancer lines with varying levels of YAP expression (Supplemental Fig." . _:b546166464 "4"^^ . . _:b546166584 . _:b29203202 . _:b546166467 "4"^^ . . _:b546166563 . . _:b546166466 "4"^^ . _:b29203204 "Crossing the TRE-YAP strain with the mammary-specific MMTV-rtTA driver (Gunther et al. >>2002<<) allowed mammary-specific YAP overexpression in a doxycycline-regulated manner (Supplemental Fig." . . _:b546166477 "4"^^ . _:b546166490 . _:b546166671 . _:b546166736 . . _:b546166476 "4"^^ . . _:b546166479 "4"^^ . . . _:b546166478 "4"^^ . . . _:b546166648 . _:b546166473 "4"^^ . _:b546166663 . _:b546166472 "4"^^ . _:b546166396 . _:b546166719 . _:b546166475 "4"^^ . . _:b546166474 "4"^^ . _:b546166645 . _:b546166576 . _:b546166485 "4"^^ . _:b546166484 "4"^^ . . _:b546166487 "4"^^ . . . _:b546166592 . . _:b546166507 . _:b546166486 "4"^^ . _:b546166481 "4"^^ . _:b546166704 . _:b546166533 . . _:b546166480 "4"^^ . _:b546166483 "4"^^ . _:b546166642 . . _:b546166815 . _:b546166482 "4"^^ . . . _:b546166544 . _:b546166493 "4"^^ . _:b546166879 . _:b546166614 . _:b546166826 . _:b546166429 . _:b546166492 "4"^^ . _:b546166495 "4"^^ . . . _:b546166494 "4"^^ . . _:b546166466 . _:b546166489 "4"^^ . _:b546166817 . . _:b546166672 . _:b546166488 "4"^^ . _:b546166708 . _:b546166377 . _:b546166491 "4"^^ . _:b546166609 . _:b546166500 . _:b546166774 . . _:b546166490 "4"^^ . . _:b546166761 . . _:b546166501 "4"^^ . _:b546166850 . _:b546166553 . . _:b546166825 . . _:b546166500 "4"^^ . . _:b546166503 "4"^^ . _:b546166653 . _:b546166640 . . _:b546166502 "4"^^ . _:b546166689 . _:b546166497 "4"^^ . . _:b546166806 . _:b546166610 . _:b546166676 . _:b546166362 . _:b546166496 "4"^^ . _:b546166499 "4"^^ . . _:b546166498 "4"^^ . . _:b546166509 "4"^^ . _:b546166437 . _:b546166508 "4"^^ . . . _:b546166511 "4"^^ . _:b546166499 . . _:b546166510 "4"^^ . _:b546166351 . . . _:b546166505 "4"^^ . _:b546166734 . . _:b546166504 "4"^^ . _:b546166383 . _:b546166507 "4"^^ . _:b546166573 . _:b546166506 "4"^^ . . _:b546166517 "3"^^ . _:b546166516 "3"^^ . _:b546166519 "3"^^ . _:b546166716 . . _:b546166518 "3"^^ . _:b546166711 . _:b546166513 "4"^^ . _:b546166749 . . . _:b546166512 "4"^^ . _:b546166369 . _:b546166515 "3"^^ . . _:b546166791 . _:b546166514 "3"^^ . _:b546166793 . . . _:b546166525 "3"^^ . _:b546166365 . _:b546166524 "3"^^ . . . _:b546166527 "3"^^ . _:b546166763 . _:b546166526 "3"^^ . _:b546166724 . _:b546166521 "3"^^ . . _:b546166520 "3"^^ . _:b546166624 . _:b546166523 "3"^^ . . . _:b546166522 "3"^^ . _:b546166533 "3"^^ . . _:b546166532 "3"^^ . . _:b546166535 "3"^^ . _:b546166534 "3"^^ . _:b546166605 . _:b546166851 . . _:b546166860 . _:b546166529 "3"^^ . _:b546166528 "3"^^ . . _:b546166535 . _:b546166531 "3"^^ . _:b546166380 . _:b546166530 "3"^^ . . . _:b546166541 "3"^^ . . _:b546166540 "3"^^ . . _:b546166454 . . _:b546166702 . _:b546166543 "3"^^ . . . _:b546166842 . _:b546166542 "3"^^ . _:b546166530 . . _:b546166629 . _:b546166537 "3"^^ . . _:b546166536 "3"^^ . _:b546166604 . . . _:b546166539 "3"^^ . . _:b546166729 . . _:b546166538 "3"^^ . . _:b546166659 . _:b546166549 "3"^^ . . _:b546166548 "3"^^ . . . _:b546166551 "3"^^ . . _:b546166550 "3"^^ . _:b29203198 "2A,B; Liu et al. 1996; Long et al. 2003; Shillingford et al. >>2003<<), suggesting that YAP is required for cell survival but not terminal differentiation of mammary alveoli." . _:b546166765 . "PMC0" . _:b546166545 "3"^^ . _:b546166544 "3"^^ . _:b546166574 . . _:b546166582 . _:b546166547 "3"^^ . . _:b546166861 . _:b546166404 . _:b546166567 . _:b546166546 "3"^^ . _:b546166360 . . _:b546166557 "3"^^ . . _:b546166635 . _:b546166556 "3"^^ . _:b546166559 "3"^^ . . . _:b546166868 . _:b29203191 _:b29203192 . _:b546166558 "3"^^ . _:b546166398 . _:b29203191 _:b29203193 . . _:b29203191 _:b29203194 . _:b29203191 _:b29203195 . _:b546166533 . _:b29203191 _:b29203196 . _:b546166553 "3"^^ . _:b29203191 _:b29203197 . _:b546166581 . . _:b546166388 . _:b29203191 _:b29203198 . _:b29203191 _:b29203199 . _:b546166666 . _:b546166532 . _:b546166552 "3"^^ . _:b546166535 . _:b546166555 "3"^^ . _:b546166649 . _:b546166625 . _:b546166534 . _:b29203184 _:b29203188 . _:b546166554 "3"^^ . _:b29203184 _:b29203189 . _:b29203184 _:b29203190 . . _:b546166529 . _:b546166565 "3"^^ . _:b29203184 _:b29203185 . _:b29203184 _:b29203186 . _:b546166848 . _:b29203184 _:b29203187 . _:b546166528 . _:b546166564 "3"^^ . _:b546166531 . _:b546166567 "3"^^ . _:b546166530 . _:b546166566 "3"^^ . . _:b546166541 . . _:b546166679 . _:b546166561 "3"^^ . _:b546166386 . _:b546166540 . _:b546166560 "3"^^ . _:b546166543 . _:b546166563 "3"^^ . _:b546166690 . _:b546166542 . _:b546166562 "3"^^ . _:b546166537 . _:b546166594 . . _:b546166573 "3"^^ . _:b546166750 . _:b546166536 . _:b546166572 "3"^^ . . . _:b546166539 . _:b546166575 "3"^^ . _:b546166538 . . _:b546166574 "3"^^ . _:b546166350 . _:b29203186 "Chodosh (Gunther et al. >>2002<<). For YAP induction, transgenic and nontransgenic littermates (FVB/C57BL6 mixed background) were fed 2 mg/mL doxycycline (Sigma) in drinking water supplemented with 2.5% sucrose starting at birth." . _:b546166549 . _:b546166712 . _:b546166491 . _:b546166569 "3"^^ . _:b29203200 . _:b546166548 . _:b546166858 . _:b546166568 "3"^^ . _:b546166418 . _:b546166596 . _:b546166551 . _:b546166571 "3"^^ . _:b546166568 . . _:b546166550 . . _:b546166759 . _:b546166570 "3"^^ . _:b546166347 . _:b546166504 . _:b546166545 . _:b546166581 "3"^^ . _:b546166544 . _:b546166580 "3"^^ . _:b546166406 . _:b546166547 . _:b546166583 "3"^^ . . _:b546166465 . _:b546166546 . _:b546166582 "3"^^ . . _:b546166557 . _:b29203205 "Expression of the polyoma middle T antigen (PyMT) under the control of the MMTV promoter results in early onset mammary tumors and lung metastases in virgin mice (Guy et al. >>1992<<). Interestingly, PyMT-induced mammary tumors showed increased expression of YAP (Fig. 4A) and TAZ (Supplemental Fig. S6A). To examine whether YAP contributes to PyMT-induced breast cancer, we inactivated YAP by introducing MMTV-Cre;" . . _:b546166869 . _:b546166577 "3"^^ . . _:b546166556 . _:b546166576 "3"^^ . _:b546166559 . _:b546166579 "3"^^ . . _:b546166558 . _:b546166424 . _:b546166578 "3"^^ . . _:b546166593 . . _:b546166553 . _:b546166589 "3"^^ . . _:b546166552 . _:b546166588 "3"^^ . . _:b546166555 . _:b546166591 "3"^^ . _:b546166853 . . _:b546166877 . _:b546166554 . . . _:b546166590 "3"^^ . . _:b546166565 . _:b546166585 "3"^^ . _:b546166611 . _:b546166564 . _:b546166584 "3"^^ . . . _:b546166567 . _:b546166587 "3"^^ . . _:b546166566 . _:b546166586 "3"^^ . _:b546166561 . _:b546166392 . _:b546166597 "3"^^ . _:b546166560 . _:b546166753 . . _:b546166596 "3"^^ . _:b546166739 . _:b546166563 . _:b546166599 "3"^^ . _:b546166562 . . _:b546166598 "3"^^ . _:b546166623 . _:b29203193 "in the virgin gland; proliferation, differentiation, and the formation of lobuloalveolar structures during pregnancy; and massive epithelial cell death in post-lactation-induced involution (Richert et al. 2000; Brisken and Rajaram >>2006<<; Watson and Khaled 2008). We first examined the temporal and spatial patterns of YAP expression by immunohistochemistry." . _:b546166573 . _:b546166593 "3"^^ . _:b546166408 . _:b546166835 . _:b546166572 . . _:b546166639 . _:b546166592 "3"^^ . _:b546166741 . _:b546166782 . _:b546166575 . _:b546166595 "3"^^ . . . . _:b546166808 . _:b546166574 . _:b546166594 "3"^^ . _:b546166569 . _:b546166605 "2"^^ . _:b29203199 . . _:b546166568 . _:b546166604 "2"^^ . _:b546166558 . . _:b546166571 . _:b546166607 "2"^^ . . _:b546166402 . _:b546166606 "2"^^ . _:b546166570 . _:b546166559 . . . _:b546166581 . _:b546166601 "3"^^ . _:b546166580 . _:b546166752 . _:b546166600 "3"^^ . _:b546166583 . . _:b546166603 "3"^^ . _:b546166696 . _:b546166582 . _:b546166602 "3"^^ . . _:b546166577 . _:b546166613 "2"^^ . _:b546166431 . _:b546166446 . _:b546166576 . _:b546166612 "2"^^ . _:b546166833 . . _:b546166579 . _:b546166615 "2"^^ . . _:b546166560 . _:b546166578 . _:b546166614 "2"^^ . . _:b546166589 . _:b546166609 "2"^^ . _:b546166588 . _:b546166608 "2"^^ . . _:b546166836 . _:b546166611 "2"^^ . _:b546166591 . . . _:b546166590 . _:b546166610 "2"^^ . . _:b546166585 . _:b546166621 "2"^^ . _:b546166619 . . _:b546166620 "2"^^ . _:b546166584 . . _:b546166675 . _:b546166587 . _:b546166623 "2"^^ . _:b546166714 . . _:b546166622 "2"^^ . _:b546166586 . . . _:b546166617 "2"^^ . _:b546166597 . . _:b546166596 . _:b546166616 "2"^^ . _:b546166448 . . _:b546166837 . _:b546166599 . _:b546166619 "2"^^ . . _:b546166618 "2"^^ . _:b546166598 . _:b546166779 . _:b546166755 . _:b546166593 . _:b546166629 "2"^^ . . . _:b546166628 "2"^^ . _:b546166592 . _:b546166442 . . . _:b546166595 . _:b546166631 "2"^^ . _:b546166389 . _:b546166594 . _:b546166630 "2"^^ . _:b546166445 . _:b546166695 . _:b546166341 . _:b546166605 . _:b546166625 "2"^^ . . . . _:b546166634 . _:b546166624 "2"^^ . _:b546166604 . _:b546166607 . _:b546166627 "2"^^ . . _:b546166606 . _:b546166626 "2"^^ . . _:b546166637 "2"^^ . _:b546166601 . . _:b546166769 . _:b546166600 . _:b546166636 "2"^^ . . _:b546166515 . _:b546166539 . _:b546166639 "2"^^ . _:b546166603 . . _:b546166700 . . _:b546166348 . _:b546166867 . _:b546166602 . _:b546166638 "2"^^ . _:b546166494 . _:b546166472 . . _:b546166633 "2"^^ . _:b546166613 . . _:b546166511 . . _:b546166612 . _:b546166632 "2"^^ . _:b546166598 . _:b546166615 . _:b546166635 "2"^^ . _:b546166614 . _:b546166634 "2"^^ . _:b546166804 . _:b546166626 . _:b546166609 . _:b546166645 "2"^^ . . . _:b546166401 . _:b546166644 "2"^^ . _:b546166608 . _:b546166722 . _:b546166611 . _:b546166647 "2"^^ . _:b546166610 . _:b546166646 "2"^^ . . _:b546166621 . _:b29203194 "proliferation, differentiation, and the formation of lobuloalveolar structures during pregnancy; and massive epithelial cell death in post-lactation-induced involution (Richert et al. 2000; Brisken and Rajaram 2006; Watson and Khaled >>2008<<). We first examined the temporal and spatial patterns of YAP expression by immunohistochemistry." . _:b546166754 . _:b546166742 . _:b546166641 "2"^^ . . _:b546166633 . _:b546166620 . _:b546166640 "2"^^ . _:b546166623 . _:b546166643 "2"^^ . . . _:b546166642 "2"^^ . _:b546166622 . _:b546166691 . _:b546166395 . _:b546166617 . _:b546166743 . _:b546166653 "2"^^ . _:b546166699 . . _:b546166652 "2"^^ . _:b546166616 . _:b546166476 . . _:b546166619 . _:b546166655 "2"^^ . _:b546166630 . . . . _:b546166654 "2"^^ . _:b546166618 . . _:b546166629 . _:b546166649 "2"^^ . . _:b546166628 . _:b546166648 "2"^^ . _:b546166531 . _:b546166342 . _:b546166566 . _:b546166631 . _:b546166651 "2"^^ . _:b546166449 . _:b546166832 . _:b546166650 "2"^^ . _:b546166630 . _:b546166833 . _:b546166834 . . _:b546166835 . . _:b546166836 . _:b546166661 "2"^^ . _:b546166625 . _:b546166837 . _:b546166838 . _:b546166492 . _:b546166839 . _:b546166840 . _:b546166805 . _:b546166660 "2"^^ . _:b546166624 . _:b546166841 . _:b546166842 . _:b546166843 . . _:b546166844 . _:b546166663 "2"^^ . _:b546166627 . _:b546166845 . _:b546166846 . _:b546166847 . _:b546166816 . _:b546166662 "2"^^ . _:b546166626 . . _:b546166817 . _:b546166818 . _:b29203206 . _:b546166819 . _:b546166820 . _:b546166657 "2"^^ . _:b546166637 . _:b546166821 . _:b546166737 . _:b546166822 . . _:b546166823 . _:b546166824 . _:b546166656 "2"^^ . _:b546166636 . _:b546166825 . . _:b546166826 . _:b546166827 . _:b546166828 . _:b546166659 "2"^^ . _:b546166639 . . _:b546166416 . _:b546166829 . _:b546166830 . _:b546166831 . _:b546166800 . _:b546166658 "2"^^ . _:b546166638 . _:b546166801 . _:b546166802 . . . _:b546166361 . _:b546166803 . _:b546166804 . _:b546166669 "2"^^ . _:b546166633 . _:b546166805 . _:b546166501 . _:b546166806 . _:b29203190 "Although this MMTV-Cre line was recently reported to show lactation defects resulting in the death of all pups before weaning (Robinson and Hennighausen >>2011<<), in our colony, these mice (in a FVB/129/C57Bl6 mixed background) can rear 50% of pups to viable adults, and examination of mammary glands at 18.5 d of pregnancy reveals no histological difference between wild-type and MMTV-Cre mice." . _:b546166807 . _:b546166808 . _:b546166668 "2"^^ . _:b546166632 . _:b546166809 . _:b546166810 . _:b546166811 . _:b546166379 . _:b546166812 . _:b546166671 "2"^^ . _:b546166635 . _:b546166813 . _:b546166814 . _:b546166617 . . _:b546166815 . _:b546166784 . _:b29203189 . _:b546166670 "2"^^ . _:b546166634 . _:b546166785 . _:b546166786 . _:b546166787 . . _:b546166788 . _:b546166665 "2"^^ . _:b546166645 . _:b546166789 . _:b546166453 . _:b546166790 . _:b546166657 . _:b546166791 . _:b546166792 . _:b546166664 "2"^^ . _:b546166644 . _:b546166793 . _:b546166794 . _:b546166795 . . _:b546166667 "2"^^ . _:b546166647 . _:b546166796 . _:b546166797 . _:b546166798 . _:b546166799 . . _:b546166666 "2"^^ . _:b546166646 . _:b546166641 . _:b546166677 "2"^^ . . _:b546166767 . _:b546166640 . _:b546166676 "2"^^ . _:b546166549 . _:b546166371 . _:b546166679 "2"^^ . _:b546166643 . . . _:b546166880 . _:b546166875 . _:b546166678 "2"^^ . _:b546166642 . . _:b546166653 . _:b546166673 "2"^^ . . . _:b546166652 . _:b546166672 "2"^^ . _:b546166572 . _:b546166655 . _:b546166675 "2"^^ . _:b546166373 . _:b546166864 . _:b546166674 "2"^^ . _:b546166654 . _:b546166865 . _:b546166866 . _:b546166867 . _:b546166735 . _:b546166868 . _:b546166685 "2"^^ . _:b546166669 . _:b546166649 . _:b546166587 . _:b546166869 . _:b546166583 . _:b546166870 . _:b546166871 . _:b546166872 . _:b29203195 . _:b546166684 "2"^^ . _:b546166648 . . _:b546166873 . _:b546166874 . _:b546166875 . _:b546166686 . _:b546166876 . _:b546166687 "2"^^ . _:b546166651 . _:b546166459 . . _:b29203186 . _:b546166877 . . _:b546166878 . _:b546166879 . _:b546166848 . _:b546166686 "2"^^ . _:b546166650 . _:b546166849 . _:b546166850 . _:b546166851 . _:b546166403 . . _:b546166852 . _:b29203197 "2A,B; Liu et al. 1996; Long et al. >>2003<<; Shillingford et al. 2003), suggesting that YAP is required for cell survival but not terminal differentiation of mammary alveoli." . _:b546166681 "2"^^ . _:b546166661 . _:b546166853 . . _:b546166854 . _:b546166409 . _:b546166855 . _:b546166856 . _:b546166680 "2"^^ . _:b546166660 . _:b546166857 . _:b546166858 . _:b546166859 . . _:b546166860 . _:b546166683 "2"^^ . _:b546166663 . _:b546166661 . _:b546166861 . _:b546166862 . _:b546166863 . _:b546166718 . _:b546166662 . _:b546166682 "2"^^ . _:b546166417 . _:b546166426 . _:b546166473 . _:b546166657 . _:b546166693 "2"^^ . _:b546166656 . _:b546166692 "2"^^ . _:b546166659 . _:b546166695 "2"^^ . . _:b546166757 . . _:b546166694 "2"^^ . _:b546166658 . _:b546166407 . _:b546166488 . _:b546166669 . _:b546166689 "2"^^ . _:b546166668 . _:b546166688 "2"^^ . _:b546166671 . _:b546166691 "2"^^ . . _:b546166372 . _:b546166670 . _:b546166690 "2"^^ . _:b546166665 . _:b546166701 "2"^^ . _:b546166745 . _:b546166803 . . _:b546166664 . _:b546166700 "2"^^ . . _:b546166508 . _:b546166667 . _:b546166703 "2"^^ . . . _:b546166702 "2"^^ . _:b546166666 . _:b546166551 . _:b546166643 . _:b546166677 . _:b546166697 "2"^^ . . . _:b546166807 . _:b546166696 "2"^^ . _:b546166676 . _:b546166679 . _:b546166699 "2"^^ . . _:b546166482 . _:b546166620 . _:b546166698 "2"^^ . _:b546166678 . _:b546166673 . _:b546166709 "2"^^ . . _:b546166480 . . _:b546166532 . . _:b546166708 "2"^^ . _:b546166672 . . . _:b546166711 "2"^^ . _:b546166675 . . . . _:b546166834 . _:b546166710 "2"^^ . _:b546166674 . . _:b546166789 . _:b546166608 . _:b546166439 . _:b546166705 "2"^^ . _:b546166685 . _:b546166788 . _:b546166518 . _:b546166684 . _:b546166704 "2"^^ . _:b546166791 . . _:b546166687 . _:b546166788 . _:b546166707 "2"^^ . _:b546166871 . . _:b546166790 . _:b546166686 . _:b546166823 . _:b546166706 "2"^^ . _:b546166616 . _:b546166785 . _:b546166681 . _:b546166717 "2"^^ . _:b546166764 . _:b546166784 . _:b546166545 . _:b546166680 . _:b546166716 "2"^^ . . _:b546166822 . _:b546166787 . . . _:b546166683 . _:b29203198 . _:b546166719 "2"^^ . _:b546166470 . _:b546166786 . . _:b546166718 "2"^^ . _:b546166682 . _:b546166368 . _:b546166797 . . _:b546166420 . _:b546166607 . _:b546166713 "2"^^ . _:b546166693 . _:b546166391 . . . _:b546166796 . _:b546166588 . _:b546166674 . _:b546166692 . _:b546166712 "2"^^ . . _:b546166799 . _:b546166461 . _:b546166715 "2"^^ . _:b546166695 . . _:b546166798 . _:b546166694 . _:b546166714 "2"^^ . _:b546166793 . _:b546166713 . _:b546166689 . _:b546166820 . _:b546166725 "2"^^ . . _:b546166792 . . . _:b546166724 "2"^^ . _:b546166688 . . _:b546166795 . _:b546166691 . _:b546166727 "2"^^ . _:b546166496 . _:b29203188 . _:b546166794 . _:b546166612 . _:b546166726 "2"^^ . _:b546166690 . . . . _:b29203189 . _:b546166805 . _:b546166636 . . _:b546166701 . _:b546166721 "2"^^ . _:b546166727 . _:b546166804 . _:b29203190 . . . _:b546166720 "2"^^ . _:b546166700 . _:b546166595 . _:b546166807 . . _:b29203191 . _:b546166703 . _:b546166723 "2"^^ . _:b546166806 . _:b29203184 . _:b546166521 . . _:b546166722 "2"^^ . _:b546166702 . _:b546166385 . _:b29203185 . _:b546166801 . _:b546166438 . _:b546166697 . _:b546166733 "2"^^ . . _:b29203186 . _:b546166800 . . _:b546166696 . _:b546166732 "2"^^ . _:b546166803 . _:b29203187 . _:b546166699 . _:b546166735 "2"^^ . . _:b546166802 . _:b29203196 . _:b546166440 . _:b546166734 "2"^^ . _:b546166698 . . _:b546166813 . _:b29203197 . _:b546166554 . _:b546166709 . _:b546166729 "2"^^ . _:b546166812 . _:b29203198 . . . _:b546166728 "2"^^ . _:b546166708 . . _:b29203199 . _:b546166815 . _:b546166711 . _:b546166731 "2"^^ . _:b546166814 . _:b29203192 . . _:b546166730 "2"^^ . _:b546166710 . . _:b29203193 . _:b546166809 . . _:b546166741 "2"^^ . _:b546166705 . _:b546166808 . _:b29203194 . . . _:b546166704 . _:b546166740 "2"^^ . _:b546166345 . _:b29203195 . _:b546166811 . _:b546166707 . _:b29203207 "Consistent with this view, the YAP amplicon in mammary tumors was isolated from a genetic background bearing loss of Brca1 and p53 tumor suppressor genes (Overholtzer et al. >>2006<<). Second, the Hippo pathway is dispensable in virgin glands but specifically required during pregnancy, when loss of YAP causes apoptosis of mammary epithelial cells, and hyperactivation of YAP results in a failure of terminal" . _:b546166743 "2"^^ . _:b546166810 . . _:b546166742 "2"^^ . _:b546166706 .