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introduction
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At present, lung cancer is the leading cause of cancer-associated mortality worldwide in men and women (>>1<<). Despite the discovery and application of new standard therapies, the current 5-year relative survival rate for lung cancer remains at 18% (1).
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Despite the discovery and application of new standard therapies, the current 5-year relative survival rate for lung cancer remains at 18% (>>1<<). Lung cancer is classified into two major categories according to pathological type: Non-small cell lung cancer (NSCLC) and small cell carcinoma. NSCLC, the most common type of lung cancer, accounts for ~80–85% of all lung cancer cases
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NSCLC, the most common type of lung cancer, accounts for ~80–85% of all lung cancer cases (>>2<<). The disease stage at diagnosis, including the T stage, has been reported to predict the prognosis of NSCLC (3). However, the effects of gene expression on tumor growth in NSCLC remain far from being completely understood.
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The disease stage at diagnosis, including the T stage, has been reported to predict the prognosis of NSCLC (>>3<<). However, the effects of gene expression on tumor growth in NSCLC remain far from being completely understood.
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CD44, a 85–90 kDa transmembrane glycoprotein, is widely expressed on the surface of cells in the majority of normal and carcinomatous human tissues (>>4<<). In normal human cells, CD44 has been reported to be involved in multitudinous cellular functions, including proliferation, adhesion, migration, hematopoiesis, lymphocyte activation, homing and extravasation (5).
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In normal human cells, CD44 has been reported to be involved in multitudinous cellular functions, including proliferation, adhesion, migration, hematopoiesis, lymphocyte activation, homing and extravasation (>>5<<). In various types of human malignancy, high levels of CD44 expression have been demonstrated to be associated with cancer progression (6–10). In lung cancer, diverse studies have clarified that the expression of CD44 is associated with
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In various types of human malignancy, high levels of CD44 expression have been demonstrated to be associated with cancer progression (>>6<<–10). In lung cancer, diverse studies have clarified that the expression of CD44 is associated with tumorigenesis and malignant features of NSCLC (11,12). Tumor and serum expression of CD44 have been confirmed as prognostic indicators for
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In lung cancer, diverse studies have clarified that the expression of CD44 is associated with tumorigenesis and malignant features of NSCLC (>>11<<,12). Tumor and serum expression of CD44 have been confirmed as prognostic indicators for patients with NSCLC (13,14). In addition, overexpression of CD44 is associated with the occurrence and migration of NSCLC (15). Furthermore,
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In lung cancer, diverse studies have clarified that the expression of CD44 is associated with tumorigenesis and malignant features of NSCLC (11,>>12<<). Tumor and serum expression of CD44 have been confirmed as prognostic indicators for patients with NSCLC (13,14). In addition, overexpression of CD44 is associated with the occurrence and migration of NSCLC (15). Furthermore,
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Tumor and serum expression of CD44 have been confirmed as prognostic indicators for patients with NSCLC (>>13<<,14). In addition, overexpression of CD44 is associated with the occurrence and migration of NSCLC (15). Furthermore, accumulating evidence has demonstrated that lung cancer cells expressing CD44 tend to have stem cell-like properties
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Tumor and serum expression of CD44 have been confirmed as prognostic indicators for patients with NSCLC (13,>>14<<). In addition, overexpression of CD44 is associated with the occurrence and migration of NSCLC (15). Furthermore, accumulating evidence has demonstrated that lung cancer cells expressing CD44 tend to have stem cell-like properties (16–18)
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In addition, overexpression of CD44 is associated with the occurrence and migration of NSCLC (>>15<<). Furthermore, accumulating evidence has demonstrated that lung cancer cells expressing CD44 tend to have stem cell-like properties (16–18) and that cancer stem cells are responsible for driving metastasis (16). CD44 also contributes to
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Furthermore, accumulating evidence has demonstrated that lung cancer cells expressing CD44 tend to have stem cell-like properties (>>16<<–18) and that cancer stem cells are responsible for driving metastasis (16).
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Furthermore, accumulating evidence has demonstrated that lung cancer cells expressing CD44 tend to have stem cell-like properties (16–18) and that cancer stem cells are responsible for driving metastasis (>>16<<). CD44 also contributes to drug resistance in lung cancer (19). Although evidence demonstrating that CD44 may act as an oncogene in NSCLC exists, direct cell experiments are still required to further confirm the effect of CD44 on the
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CD44 also contributes to drug resistance in lung cancer (>>19<<). Although evidence demonstrating that CD44 may act as an oncogene in NSCLC exists, direct cell experiments are still required to further confirm the effect of CD44 on the malignant phenotype of lung cancer cells.
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n3:24091605
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dc:title
materials and methods
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Sections were routinely stained, as described in our previous study (>>20<<). Briefly, the sections were immersed in xylene to remove the paraffin, washed in a graded series of ethanol, immersed in citrate buffer and then incubated in a high-pressure sterilization oven for antigen retrieval with citrate buffer at
n2:mentions
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The relative gene expression levels were calculated by the 2−ΔΔCq method (where ΔCq=Cqtarget- Cqcontrol) (>>21<<).
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n3:11846609
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results
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There was no significant difference between CD44 expression and age, sex, smoking status, pathology type, N classification or TNM stage (>>22<<). However, the level of CD44 expression was positively associated with an advanced T classification (P<0.05; Table I). A total of 58.9% of cases (33 of 56) stained positive for CD44 in the T1 group, while 80.0% of cases (28 of 35) stained
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discussion
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In previous studies, high expression of CD44 was hypothesized to be associated with poor prognosis in NSCLC (>>14<<,23). High expression of CD44 was also reported to be associated with not only occurrence and migration (15) but also metastasis (24) and drug resistance (19) of NSCLC.
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In previous studies, high expression of CD44 was hypothesized to be associated with poor prognosis in NSCLC (14,>>23<<). High expression of CD44 was also reported to be associated with not only occurrence and migration (15) but also metastasis (24) and drug resistance (19) of NSCLC.
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High expression of CD44 was also reported to be associated with not only occurrence and migration (>>15<<) but also metastasis (24) and drug resistance (19) of NSCLC.
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High expression of CD44 was also reported to be associated with not only occurrence and migration (15) but also metastasis (>>24<<) and drug resistance (19) of NSCLC.
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High expression of CD44 was also reported to be associated with not only occurrence and migration (15) but also metastasis (24) and drug resistance (>>19<<) of NSCLC.
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In the present study, an association between a higher CD44-positive expression rate and higher T stage was observed in NSCLC, which was consistent with the report from Shinohara et al (>>14<<) where significant associations were observed between CD44 expression and clinicopathological factors including T stage, N stage, pathological stage and histological type following immunohistochemical analysis of a cohort consisting of
n2:mentions
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were observed between CD44 expression and clinicopathological factors including T stage, N stage, pathological stage and histological type following immunohistochemical analysis of a cohort consisting of 261 consecutive patients (>>12<<), but the present study failed to identify a significant association between CD44 expression and occurrence or metastasis.
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There are also certain reports indicating that CD44 variant 6 (CD44v6) is associated with disease progression in NSCLC (>>13<<,25,26). Luo et al (23) reported that high expression of CD44v6 was associated with histopathological type and lymph node metastasis by using meta-analysis for a total of 921 patients with NSCLC from ten studies. However, whether the
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There are also certain reports indicating that CD44 variant 6 (CD44v6) is associated with disease progression in NSCLC (13,>>25<<,26). Luo et al (23) reported that high expression of CD44v6 was associated with histopathological type and lymph node metastasis by using meta-analysis for a total of 921 patients with NSCLC from ten studies. However, whether the
n2:mentions
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There are also certain reports indicating that CD44 variant 6 (CD44v6) is associated with disease progression in NSCLC (13,25,>>26<<). Luo et al (23) reported that high expression of CD44v6 was associated with histopathological type and lymph node metastasis by using meta-analysis for a total of 921 patients with NSCLC from ten studies. However, whether the expression
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Luo et al (>>23<<) reported that high expression of CD44v6 was associated with histopathological type and lymph node metastasis by using meta-analysis for a total of 921 patients with NSCLC from ten studies.
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These results were constant with those of a previous report, where purified CD44-positive cells were demonstrated to have higher tumorigenicity than negative cells in vitro and in vivo (>>17<<). With increasing research focusing on the function of CD44 in cancer, it is generally accepted that CD44 is a prime therapeutic target for cancer interventions (27). The present study supports the hypothesis that CD44 is a potential
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With increasing research focusing on the function of CD44 in cancer, it is generally accepted that CD44 is a prime therapeutic target for cancer interventions (>>27<<). The present study supports the hypothesis that CD44 is a potential therapeutic target for NSCLC. Inhibition of CD44 expression, either by siRNAs or by CRISPR/Cas9 gene editing targeting NSCLC, may suppress lung cancer cell proliferation
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