_:b212098532 . _:b212098535 . _:b212098320 . _:b212098504 . . _:b212098534 . . _:b212098371 . _:b212098529 . _:b212098528 . _:b212098531 . . . _:b212098530 . . _:b212098541 . . . _:b212098540 . _:b212098304 . _:b212098157 . _:b212098543 . _:b84235 . _:b212098295 . _:b212098542 . . _:b212098537 . _:b84229 "The peptide sequences SLLMWITQCFL, SLLMWITQC, and QLSLLMWIT are located between positions 155 and 167 of the NY-ESO-1 protein (>>15<<), and show overlapping sequences." . _:b212098536 . _:b212098539 . _:b212098280 . _:b212098423 . _:b212098386 . _:b212098538 . _:b212098298 . _:b212098549 . . . _:b212098505 . _:b212098548 . _:b212098456 . _:b212098316 . _:b212098551 . _:b212098476 . _:b212098550 . _:b212098545 . . _:b212098159 . . _:b212098544 . . . _:b212098489 . . _:b212098547 . _:b212098546 . _:b212098449 . . _:b212098557 . . _:b212098556 . . _:b212098387 . _:b212098559 . . . _:b212098558 . _:b212098271 . . _:b212098553 . . _:b212098552 . . _:b84230 _:b84240 . _:b84230 _:b84241 . _:b84230 _:b84242 . _:b212098555 . _:b84230 _:b84243 . _:b84230 _:b84236 . _:b84228 _:b84229 . "PMC0" . _:b84230 _:b84237 . _:b84230 _:b84238 . _:b212098554 . _:b212098418 . _:b212098398 . _:b84230 _:b84239 . . _:b84230 _:b84232 . _:b84230 _:b84233 . _:b84230 _:b84234 . _:b84230 _:b84235 . _:b212098286 . . _:b212098390 . _:b212098254 . _:b212098291 . _:b84230 _:b84231 . . . _:b212098441 . _:b212098201 . _:b212098455 . _:b212098207 . _:b212098359 . _:b212098487 . _:b212098180 . _:b212098514 . . _:b212098208 . . _:b84235 "Using specific antitumor CTLs as probes, a number of CTL targets have been cloned on the basis of MHC class I\u2013restricted recognition (>>1<<, 6). However, this approach involves cultured cancer cell lines and stable CTL lines from the same patient, two requirements that cannot easily be met with many tumor types. With the demonstration that genes coding for CTL-recognized" . . . . . . . _:b212098327 . _:b212098310 . _:b212098472 . . . . . . _:b212098385 . . _:b84243 . . _:b212098444 . _:b84242 "(25), and difficult to interpret in trials with vaccines containing melanocyte differentiation antigens, since CTLs against these antigens can be generated in vitro from nonvaccinated melanoma patients as well as normal individuals (26, >>27<<). However, de novo induction and increase of preexisting CTL reactivity have been detected after vaccination with melanocyte differentiation antigens and observed to be associated with cancer regressions in a limited number of patients" . _:b212098288 . _:b212098493 . . . _:b212098508 . _:b212098213 . _:b212098222 . _:b212098417 . _:b212098246 . _:b212098529 . . . . . _:b212098540 . _:b212098290 . . . _:b212098287 . . _:b84222 . _:b212098510 . _:b212098285 . . _:b212098518 . _:b212098462 . _:b212098336 . . _:b212098256 . _:b212098445 . _:b212098509 . . _:b212098370 . . . . . _:b212098498 . _:b212098277 . _:b212098486 . _:b212098405 . . _:b212098243 . . . _:b212098499 . _:b212098555 . . . . _:b212098161 . _:b212098324 . . . _:b84230 "discussion" . _:b212098451 . _:b84223 . _:b212098526 . . . . _:b212098517 . _:b212098212 . . _:b212098410 . . . _:b212098301 . . _:b212098303 . . _:b212098361 . _:b212098265 . . _:b212098560 . _:b212098273 . . _:b212098545 . . _:b212098331 . . . _:b212098159 "13"^^ . . . _:b212098181 . _:b212098534 . . _:b212098560 "2"^^ . _:b212098156 "19"^^ . . _:b84238 "A number of novel tumor antigens have been defined by SEREX, including two new members of the cancer\u2013testis antigenic family, SSX2 (HOM-MEL-40) (5, >>16<<), and NY-ESO-1 (15)." . _:b212098184 . _:b212098157 "17"^^ . _:b212098158 "14"^^ . . _:b212098438 . _:b84237 "A number of novel tumor antigens have been defined by SEREX, including two new members of the cancer\u2013testis antigenic family, SSX2 (HOM-MEL-40) (>>5<<, 16), and NY-ESO-1 (15)." . _:b212098166 "9"^^ . _:b212098167 "9"^^ . . _:b212098164 "9"^^ . _:b212098205 . . _:b212098165 "9"^^ . _:b212098319 . _:b212098163 "10"^^ . _:b212098162 "10"^^ . . _:b212098466 . _:b212098483 . . _:b212098161 "11"^^ . _:b212098160 "11"^^ . _:b212098174 "7"^^ . . _:b212098175 "6"^^ . _:b212098172 "7"^^ . . _:b212098173 "7"^^ . . _:b212098170 "8"^^ . _:b212098380 . _:b212098171 "7"^^ . _:b212098340 . _:b212098168 "9"^^ . _:b212098169 "8"^^ . _:b212098180 "6"^^ . _:b84234 "An autologous typing system also provided the first evidence for the development of CTLs with specificity for human melanoma cells (3, 17, >>21<<\u201324). Using specific antitumor CTLs as probes, a number of CTL targets have been cloned on the basis of MHC class I\u2013restricted recognition (1, 6). However, this approach involves cultured cancer cell lines and stable CTL lines from the" . _:b212098183 "5"^^ . _:b212098181 "6"^^ . . _:b212098182 "5"^^ . _:b212098178 "6"^^ . _:b212098179 "6"^^ . _:b212098176 "6"^^ . . _:b212098177 "6"^^ . . _:b212098157 . . _:b212098226 . _:b212098189 "5"^^ . . _:b212098156 . _:b212098188 "5"^^ . _:b212098159 . _:b212098191 "5"^^ . _:b212098158 . _:b212098422 . . _:b212098190 "5"^^ . _:b212098516 . . _:b212098560 . . . _:b212098185 "5"^^ . . _:b84221 _:b84224 . _:b212098184 "5"^^ . _:b84221 _:b84225 . . _:b212098299 . _:b84221 _:b84226 . _:b84221 _:b84227 . . _:b212098275 . _:b212098187 "5"^^ . . _:b212098253 . _:b212098186 "5"^^ . . _:b212098165 . _:b212098197 "5"^^ . _:b212098164 . . _:b212098196 "5"^^ . _:b212098167 . _:b212098199 "4"^^ . _:b212098350 . _:b212098166 . _:b212098198 "4"^^ . . _:b212098161 . . _:b212098193 "5"^^ . _:b212098259 . _:b212098160 . _:b212098192 "5"^^ . _:b212098172 . _:b212098163 . _:b212098250 . _:b212098195 "5"^^ . _:b212098162 . _:b212098194 "5"^^ . _:b212098173 . . _:b212098205 "4"^^ . . _:b212098172 . _:b212098204 "4"^^ . _:b212098376 . _:b212098464 . . _:b212098175 . _:b212098207 "4"^^ . . _:b212098174 . _:b212098206 "4"^^ . . _:b212098169 . _:b212098201 "4"^^ . _:b212098168 . _:b212098200 "4"^^ . _:b212098191 . _:b212098362 . _:b212098171 . _:b212098203 "4"^^ . _:b212098372 . _:b212098170 . _:b212098202 "4"^^ . _:b212098228 . _:b212098181 . _:b212098213 "4"^^ . . _:b212098281 . _:b212098556 . _:b212098180 . _:b212098212 "4"^^ . . . . _:b212098183 . _:b212098215 "4"^^ . . _:b212098156 . _:b212098182 . _:b212098214 "4"^^ . . _:b212098177 . _:b212098209 "4"^^ . . _:b212098240 . _:b212098176 . _:b212098208 "4"^^ . _:b212098179 . _:b212098538 . _:b212098211 "4"^^ . _:b212098178 . _:b212098210 "4"^^ . . _:b212098189 . _:b212098221 "4"^^ . _:b212098188 . _:b212098220 "4"^^ . _:b212098203 . _:b212098191 . _:b212098223 "4"^^ . _:b212098190 . _:b212098222 "4"^^ . _:b212098185 . _:b212098217 "4"^^ . _:b212098536 . _:b212098184 . _:b212098216 "4"^^ . _:b212098482 . _:b212098232 . _:b212098187 . _:b212098219 "4"^^ . _:b212098186 . _:b212098218 "4"^^ . _:b212098229 "3"^^ . _:b212098197 . _:b212098228 "3"^^ . _:b212098196 . _:b212098194 . . _:b212098231 "3"^^ . _:b212098199 . _:b212098230 "3"^^ . _:b212098198 . _:b212098225 "3"^^ . _:b212098193 . . _:b212098224 "3"^^ . _:b212098192 . _:b212098312 . _:b212098550 . _:b212098227 "3"^^ . _:b212098195 . . _:b212098522 . _:b212098226 "3"^^ . _:b212098194 . _:b212098237 "3"^^ . _:b212098205 . . _:b212098236 "3"^^ . _:b212098204 . . _:b212098239 "3"^^ . _:b212098207 . _:b212098238 "3"^^ . _:b212098206 . _:b212098325 . . _:b84227 . . _:b212098233 "3"^^ . _:b212098481 . _:b212098201 . _:b84226 . _:b212098232 "3"^^ . _:b212098200 . _:b212098235 "3"^^ . _:b212098397 . _:b212098203 . _:b212098234 "3"^^ . _:b212098202 . . _:b212098213 . _:b212098245 "3"^^ . _:b212098249 . . _:b212098244 "3"^^ . _:b212098212 . _:b212098393 . _:b212098215 . . _:b212098247 "3"^^ . _:b212098214 . _:b212098246 "3"^^ . _:b212098209 . . _:b212098241 "3"^^ . . . . _:b212098208 . _:b212098240 "3"^^ . _:b212098211 . _:b212098243 "3"^^ . . . _:b212098210 . _:b212098242 "3"^^ . . _:b212098425 . _:b212098221 . _:b212098317 . _:b212098253 "3"^^ . . _:b212098220 . _:b212098252 "3"^^ . . _:b212098548 . _:b212098419 . _:b212098223 . _:b212098255 "3"^^ . _:b212098244 . _:b212098222 . _:b212098531 . . _:b212098254 "3"^^ . . _:b212098217 . _:b212098249 "3"^^ . _:b212098216 . _:b212098248 "3"^^ . . . . _:b212098219 . . _:b212098251 "3"^^ . . . _:b212098218 . _:b212098377 . _:b212098250 "3"^^ . _:b212098378 . _:b84224 "NY-ESO-1 peptides with HLA-A2\u2013binding motifs were predicted by computer analysis as described (>>19<<). Peptides were synthesized using a multiple peptide synthesizer (Abimed 422; Abimed, Langenfeld, Germany)." . _:b212098229 . _:b212098261 "3"^^ . _:b212098392 . _:b212098460 . _:b212098363 . _:b212098228 . . _:b212098260 "3"^^ . _:b212098231 . _:b212098263 "3"^^ . _:b212098494 . _:b212098230 . _:b212098262 "3"^^ . _:b212098211 . . _:b212098225 . _:b212098257 "3"^^ . _:b212098294 . _:b212098224 . _:b212098256 "3"^^ . _:b212098174 . _:b212098227 . _:b212098384 . _:b212098259 "3"^^ . . _:b84237 . _:b212098226 . _:b212098258 "3"^^ . _:b212098237 . _:b212098269 "3"^^ . . _:b212098236 . . _:b212098268 "3"^^ . . . . _:b212098239 . _:b212098271 "3"^^ . . . _:b212098238 . . _:b212098270 "3"^^ . _:b212098233 . _:b212098411 . _:b212098265 "3"^^ . _:b212098232 . _:b212098524 . _:b212098264 "3"^^ . _:b212098233 . _:b212098164 . _:b212098235 . _:b212098267 "3"^^ . . _:b212098234 . _:b212098266 "3"^^ . . _:b212098245 . _:b212098430 . . _:b212098277 "3"^^ . . _:b212098244 . _:b212098276 "3"^^ . _:b212098247 . _:b212098528 . _:b212098279 "3"^^ . _:b212098477 . _:b212098246 . _:b212098278 "3"^^ . _:b84221 "materials and methods" . . _:b212098241 . _:b212098463 . _:b212098273 "3"^^ . . _:b212098471 . _:b212098240 . _:b212098272 "3"^^ . _:b212098179 . _:b84222 "Mixed lymphocyte tumor cell cultures of PBLs and the autologous tumor cell line from patient NW38 were performed as previously described (>>17<<). The stable CTL line NW38-IVS-1 was maintained by weekly restimulation of 2\u20133 \u00D7 105 CTLs with 5 \u00D7 104 autologous melanoma cells and 2 \u00D7 105 MZ1257-EBV cells as feeders." . _:b212098206 . _:b212098243 . _:b212098427 . . _:b212098275 "3"^^ . _:b212098251 . _:b212098431 . . _:b212098274 "3"^^ . _:b212098242 . _:b212098285 "2"^^ . _:b212098253 . _:b212098300 . _:b212098284 "2"^^ . _:b212098252 . . _:b212098156 . _:b212098439 . _:b212098287 "2"^^ . _:b212098157 . _:b212098330 . _:b212098255 . _:b212098158 . _:b212098159 . _:b212098286 "2"^^ . _:b212098254 . . . _:b212098326 . _:b212098249 . _:b212098281 "3"^^ . _:b212098168 . _:b212098169 . _:b212098280 "3"^^ . _:b212098248 . _:b212098170 . _:b212098171 . _:b212098172 . _:b212098519 . _:b212098283 "2"^^ . . _:b212098173 . . _:b212098251 . _:b212098174 . . _:b212098175 . . _:b212098160 . _:b212098161 . _:b212098282 "3"^^ . _:b212098250 . _:b212098162 . _:b212098163 . _:b212098164 . _:b212098293 "2"^^ . _:b212098165 . _:b212098247 . _:b212098261 . _:b212098166 . _:b212098167 . _:b212098292 "2"^^ . _:b212098352 . _:b212098260 . _:b212098284 . . _:b212098295 "2"^^ . _:b212098263 . _:b212098166 . . . . _:b212098294 "2"^^ . _:b212098262 . . . _:b212098289 "2"^^ . _:b212098257 . _:b212098403 . _:b212098288 "2"^^ . _:b84228 "results" . _:b212098256 . _:b212098381 . . . _:b212098479 . . _:b212098291 "2"^^ . . _:b212098259 . _:b212098290 "2"^^ . _:b212098347 . _:b212098258 . _:b212098525 . _:b212098301 "2"^^ . _:b212098269 . _:b212098216 . _:b212098424 . _:b212098300 "2"^^ . _:b212098217 . _:b212098268 . _:b212098218 . _:b212098219 . _:b212098220 . _:b212098303 "2"^^ . _:b212098221 . _:b212098271 . _:b212098222 . . _:b212098223 . . _:b212098208 . _:b212098302 "2"^^ . _:b212098209 . _:b212098408 . _:b212098270 . _:b212098210 . . _:b212098211 . _:b212098212 . _:b212098297 "2"^^ . _:b212098213 . _:b212098515 . _:b212098265 . . . _:b212098214 . _:b212098215 . . _:b212098232 . _:b212098426 . _:b212098296 "2"^^ . _:b212098233 . _:b212098264 . _:b212098234 . _:b212098235 . _:b212098236 . _:b212098299 "2"^^ . _:b212098237 . _:b212098267 . _:b212098238 . _:b212098278 . _:b212098400 . . _:b212098239 . _:b212098224 . _:b212098298 "2"^^ . _:b212098225 . _:b212098266 . _:b212098226 . _:b212098227 . _:b212098228 . _:b212098302 . _:b212098309 "2"^^ . _:b212098229 . . _:b212098277 . _:b212098230 . _:b212098231 . _:b212098184 . _:b212098308 "2"^^ . _:b212098185 . _:b212098276 . _:b212098186 . . _:b212098187 . _:b212098188 . _:b212098311 "2"^^ . _:b212098189 . _:b212098279 . _:b212098190 . _:b212098191 . _:b212098176 . _:b212098310 "2"^^ . _:b212098177 . _:b212098278 . _:b212098178 . _:b212098179 . _:b212098180 . _:b212098305 "2"^^ . _:b212098181 . _:b212098273 . _:b212098182 . _:b212098183 . _:b212098200 . _:b84221 _:b84222 . _:b212098304 "2"^^ . _:b212098216 . _:b84221 _:b84223 . _:b212098272 . _:b212098201 . _:b212098202 . _:b212098454 . . _:b212098203 . _:b212098260 . _:b212098204 . _:b212098307 "2"^^ . _:b212098205 . _:b212098275 . _:b212098206 . _:b212098355 . _:b84221 . _:b212098207 . _:b212098192 . _:b212098306 "2"^^ . _:b212098193 . _:b84222 . _:b212098274 . _:b212098194 . _:b212098220 . _:b212098195 . _:b212098196 . _:b212098317 "2"^^ . _:b212098197 . _:b84223 . _:b212098285 . _:b212098198 . _:b212098199 . _:b212098353 . _:b212098280 . _:b212098316 "2"^^ . _:b212098281 . _:b212098241 . . _:b212098284 . _:b212098282 . _:b212098283 . _:b212098284 . _:b212098319 "2"^^ . _:b212098285 . _:b212098287 . _:b212098286 . _:b212098287 . _:b212098272 . _:b212098318 "2"^^ . _:b212098273 . _:b212098478 . _:b212098286 . _:b212098274 . _:b212098275 . _:b212098276 . _:b212098313 "2"^^ . _:b212098186 . _:b212098277 . _:b212098281 . _:b212098278 . _:b212098279 . _:b212098296 . _:b212098312 "2"^^ . _:b212098297 . . _:b212098280 . _:b212098298 . _:b212098299 . _:b212098300 . . _:b212098315 "2"^^ . _:b212098301 . _:b212098283 . _:b212098302 . _:b212098303 . _:b212098288 . _:b212098170 . _:b212098314 "2"^^ . _:b212098289 . _:b212098282 . _:b212098290 . _:b212098291 . _:b212098292 . _:b212098325 "2"^^ . _:b212098293 . _:b212098293 . _:b212098294 . _:b212098295 . _:b212098248 . _:b212098432 . _:b212098324 "2"^^ . _:b212098249 . _:b212098292 . _:b212098250 . _:b212098251 . _:b212098252 . _:b212098327 "2"^^ . _:b212098253 . _:b212098295 . _:b212098254 . _:b212098255 . . . _:b212098240 . _:b212098326 "2"^^ . _:b212098241 . . _:b212098294 . _:b212098242 . _:b212098243 . _:b212098244 . _:b212098321 "2"^^ . _:b212098245 . _:b212098560 . _:b212098289 . _:b212098246 . . . _:b212098247 . _:b212098264 . _:b212098320 "2"^^ . _:b212098265 . _:b212098288 . _:b212098266 . . _:b212098267 . _:b212098268 . _:b212098323 "2"^^ . _:b212098269 . _:b212098291 . _:b212098270 . _:b212098271 . . _:b212098256 . _:b212098322 "2"^^ . _:b212098257 . _:b212098290 . _:b212098258 . _:b212098259 . _:b212098260 . _:b212098333 "2"^^ . _:b212098261 . _:b212098301 . _:b212098262 . _:b212098263 . _:b212098162 . _:b212098332 "2"^^ . _:b212098344 . _:b212098345 . _:b212098300 . _:b212098346 . _:b212098347 . _:b212098348 . _:b212098335 "2"^^ . _:b212098349 . _:b212098303 . _:b212098350 . _:b212098351 . _:b212098336 . _:b212098334 "2"^^ . _:b212098337 . _:b212098302 . _:b212098338 . _:b212098339 . _:b212098227 . _:b212098340 . _:b212098329 "2"^^ . _:b212098341 . _:b212098297 . _:b212098342 . _:b212098343 . _:b212098360 . _:b212098328 "2"^^ . . _:b212098361 . _:b212098296 . _:b212098362 . _:b212098363 . . _:b212098364 . _:b212098331 "2"^^ . _:b212098365 . _:b212098394 . _:b212098299 . _:b212098366 . _:b212098367 . _:b212098352 . _:b212098511 . _:b212098330 "2"^^ . _:b212098353 . _:b212098298 . _:b212098354 . _:b212098355 . _:b212098356 . _:b212098341 "2"^^ . _:b212098357 . _:b212098358 . _:b212098359 . _:b212098312 . _:b212098474 . _:b212098340 "2"^^ . _:b212098313 . _:b212098314 . _:b212098315 . _:b212098316 . _:b212098343 "2"^^ . _:b212098317 . _:b212098318 . . _:b212098319 . _:b212098304 . _:b84221 . _:b212098342 "2"^^ . _:b212098305 . _:b212098306 . _:b212098307 . _:b212098308 . _:b212098337 "2"^^ . _:b212098309 . _:b212098310 . . _:b212098245 . . _:b212098311 . . . _:b212098336 "2"^^ . _:b212098328 . _:b212098329 . _:b212098176 . _:b212098330 . _:b212098331 . _:b212098332 . _:b212098185 . _:b212098339 "2"^^ . _:b212098333 . _:b212098334 . _:b212098335 . _:b212098342 . _:b212098320 . _:b212098338 "2"^^ . _:b212098321 . _:b212098533 . _:b212098322 . . _:b212098323 . _:b212098324 . _:b212098349 "2"^^ . _:b212098325 . _:b212098326 . _:b212098327 . _:b212098408 . _:b212098348 "2"^^ . _:b212098409 . _:b212098410 . _:b212098411 . _:b212098539 . _:b212098412 . _:b212098512 . _:b212098351 "2"^^ . _:b212098413 . _:b212098230 . _:b212098414 . _:b212098415 . _:b212098400 . _:b212098350 "2"^^ . _:b212098401 . _:b212098402 . _:b212098403 . _:b212098469 . _:b212098404 . _:b212098345 "2"^^ . _:b212098405 . . _:b212098406 . _:b212098407 . _:b212098424 . _:b212098344 "2"^^ . _:b212098425 . _:b212098426 . _:b212098427 . _:b212098428 . _:b212098347 "2"^^ . . _:b212098429 . _:b212098430 . _:b212098436 . _:b212098431 . . . _:b212098346 "2"^^ . _:b212098416 . _:b212098417 . . . _:b212098418 . _:b212098552 . . _:b212098419 . _:b212098420 . _:b212098357 "2"^^ . . _:b212098421 . _:b212098458 . _:b212098422 . _:b212098423 . _:b212098269 . _:b212098356 "2"^^ . . _:b212098376 . _:b212098160 . _:b212098377 . _:b212098468 . _:b212098378 . _:b212098379 . _:b212098380 . _:b212098359 "2"^^ . _:b212098381 . _:b212098382 . _:b212098383 . . _:b212098358 "2"^^ . . _:b212098368 . _:b212098369 . _:b212098235 . _:b212098473 . . _:b212098370 . _:b212098371 . . _:b212098372 . _:b212098353 "2"^^ . _:b212098373 . _:b212098374 . _:b212098459 . _:b212098375 . _:b212098557 . _:b212098392 . _:b212098352 "2"^^ . _:b212098168 . _:b212098307 . . _:b212098393 . _:b212098394 . _:b212098395 . _:b212098396 . _:b212098558 . _:b212098355 "2"^^ . _:b212098397 . _:b212098398 . _:b212098399 . _:b212098384 . _:b212098354 "2"^^ . _:b212098385 . _:b212098386 . _:b212098387 . _:b212098507 . _:b212098388 . _:b212098365 "2"^^ . _:b212098389 . _:b212098390 . _:b212098183 . _:b212098391 . _:b212098490 . _:b212098429 . _:b212098472 . _:b212098364 "2"^^ . _:b212098329 . _:b212098473 . _:b212098474 . _:b212098475 . _:b212098476 . _:b212098367 "2"^^ . _:b212098477 . _:b212098478 . _:b212098479 . . _:b212098464 . _:b212098366 "2"^^ . _:b212098465 . _:b212098466 . . _:b212098467 . . _:b212098306 . _:b212098468 . _:b212098361 "2"^^ . _:b212098469 . . _:b212098470 . _:b212098471 . _:b212098488 . _:b212098360 "2"^^ . _:b212098489 . _:b212098490 . _:b212098491 . _:b212098263 . _:b212098363 "2"^^ . . _:b212098492 . _:b212098493 . _:b212098494 . _:b212098484 . _:b212098437 . _:b212098368 . _:b212098495 . _:b212098480 . _:b212098362 "2"^^ . . _:b212098481 . . _:b212098482 . _:b212098483 . _:b212098484 . _:b212098373 "2"^^ . _:b212098485 . _:b212098283 . _:b212098486 . _:b212098487 . _:b212098440 . _:b84240 . _:b212098372 "2"^^ . _:b212098441 . _:b212098442 . _:b212098443 . _:b212098444 . _:b212098375 "2"^^ . _:b212098445 . _:b212098446 . . _:b212098447 . _:b212098432 . _:b212098374 "2"^^ . _:b212098433 . _:b212098297 . _:b212098434 . _:b212098435 . _:b212098436 . _:b212098369 "2"^^ . _:b212098437 . _:b212098438 . . _:b212098439 . _:b212098456 . _:b212098368 "2"^^ . _:b212098457 . _:b212098373 . _:b212098458 . _:b212098459 . _:b212098460 . _:b212098371 "2"^^ . _:b212098461 . _:b212098462 . . _:b212098463 . _:b212098448 . _:b212098370 "2"^^ . . . _:b212098449 . _:b212098450 . . _:b212098451 . _:b212098452 . _:b212098381 "2"^^ . _:b212098234 . _:b212098453 . _:b212098454 . _:b212098455 . _:b212098536 . _:b212098380 "2"^^ . _:b212098537 . _:b212098538 . _:b212098539 . _:b212098540 . _:b212098383 "2"^^ . _:b212098541 . _:b212098542 . _:b212098543 . _:b212098528 . _:b212098382 "2"^^ . _:b212098529 . . _:b212098530 . _:b212098531 . _:b212098532 . _:b212098377 "2"^^ . _:b212098533 . _:b84239 "A number of novel tumor antigens have been defined by SEREX, including two new members of the cancer\u2013testis antigenic family, SSX2 (HOM-MEL-40) (5, 16), and NY-ESO-1 (>>15<<)." . _:b212098534 . _:b212098535 . _:b212098552 . _:b212098376 "2"^^ . _:b212098553 . _:b212098554 . _:b212098555 . _:b212098556 . _:b212098379 "2"^^ . _:b212098557 . _:b212098558 . _:b212098559 . _:b212098252 . _:b212098378 "2"^^ . _:b212098544 . _:b212098545 . . _:b212098402 . _:b212098546 . _:b212098547 . _:b212098548 . _:b212098549 . _:b212098389 "2"^^ . _:b212098549 . _:b212098550 . _:b212098551 . _:b212098520 . _:b212098504 . _:b212098388 "2"^^ . . _:b212098505 . _:b212098506 . _:b212098412 . _:b212098507 . _:b212098508 . _:b212098391 "2"^^ . _:b212098509 . _:b212098510 . _:b212098511 . _:b212098496 . _:b212098390 "2"^^ . _:b212098497 . _:b212098199 . _:b212098498 . _:b212098499 . _:b212098500 . _:b212098385 "2"^^ . _:b212098501 . . . . _:b212098502 . _:b212098503 . _:b212098520 . _:b212098384 "2"^^ . _:b212098521 . _:b212098522 . _:b212098356 . _:b212098523 . _:b212098524 . _:b212098387 "2"^^ . . . _:b212098525 . _:b212098526 . _:b212098527 . _:b212098512 . _:b212098386 "2"^^ . _:b212098513 . _:b212098366 . _:b212098514 . _:b212098515 . _:b212098516 . _:b212098397 "2"^^ . _:b212098517 . _:b212098518 . _:b212098519 . _:b212098396 "2"^^ . . _:b212098399 "2"^^ . _:b84243 "However, de novo induction and increase of preexisting CTL reactivity have been detected after vaccination with melanocyte differentiation antigens and observed to be associated with cancer regressions in a limited number of patients (>>13<<). The demonstration of a simultaneous antibody and CTL response to NY-ESO-1 in the same patient suggests that serological methods may be useful in monitoring vaccine trials with NY-ESO-1 and other tumor antigens eliciting a humoral immune" . _:b212098398 "2"^^ . _:b212098393 "2"^^ . _:b212098392 "2"^^ . _:b212098506 . _:b212098395 "2"^^ . _:b84224 . . _:b212098394 "2"^^ . . . . . _:b212098405 "2"^^ . . _:b212098404 "2"^^ . _:b212098559 . _:b212098360 . _:b212098407 "2"^^ . . _:b212098406 "2"^^ . _:b212098282 . _:b212098257 . _:b212098333 . . _:b212098401 "2"^^ . _:b212098415 . _:b212098400 "2"^^ . . _:b212098403 "2"^^ . _:b212098404 . _:b212098402 "2"^^ . _:b212098210 . . _:b212098413 "2"^^ . _:b212098541 . _:b84241 "(25), and difficult to interpret in trials with vaccines containing melanocyte differentiation antigens, since CTLs against these antigens can be generated in vitro from nonvaccinated melanoma patients as well as normal individuals (>>26<<, 27). However, de novo induction and increase of preexisting CTL reactivity have been detected after vaccination with melanocyte differentiation antigens and observed to be associated with cancer regressions in a limited number of" . _:b212098412 "2"^^ . _:b212098553 . _:b212098415 "2"^^ . _:b212098379 . _:b212098413 . _:b212098414 "2"^^ . _:b212098409 "2"^^ . . _:b212098408 "2"^^ . _:b212098453 . _:b212098383 . _:b212098492 . _:b212098358 . _:b212098239 . _:b212098411 "2"^^ . . _:b212098410 "2"^^ . _:b212098421 "2"^^ . _:b212098399 . _:b212098420 "2"^^ . _:b212098423 "2"^^ . _:b212098422 "2"^^ . _:b212098501 . _:b212098417 "2"^^ . _:b84228 . _:b212098416 "2"^^ . _:b84229 . _:b212098419 "2"^^ . _:b84230 . _:b212098364 . _:b212098418 "2"^^ . _:b84231 . . . _:b212098429 "2"^^ . _:b84224 . _:b212098428 "2"^^ . _:b84225 . . . _:b212098224 . _:b84226 . _:b212098431 "2"^^ . . . _:b84227 . _:b212098430 "2"^^ . _:b212098425 "2"^^ . _:b84236 . _:b212098537 . . . . _:b84237 . _:b212098424 "2"^^ . _:b212098309 . . _:b212098427 "2"^^ . _:b84238 . . _:b212098426 "2"^^ . _:b84239 . . . _:b212098544 . _:b212098437 "2"^^ . _:b84232 . . _:b212098218 . _:b212098262 . _:b212098414 . _:b212098436 "2"^^ . _:b84233 . . _:b212098439 "2"^^ . _:b84234 . _:b212098351 . . _:b84235 . _:b212098438 "2"^^ . _:b212098313 . _:b212098188 . _:b212098433 "2"^^ . _:b212098496 . _:b212098432 "2"^^ . _:b212098272 . _:b212098435 "2"^^ . . _:b212098309 . _:b212098434 "2"^^ . . . _:b212098308 . _:b84240 . _:b212098445 "2"^^ . _:b212098214 . _:b212098374 . _:b212098264 . _:b212098311 . _:b84241 . _:b212098444 "2"^^ . . _:b212098354 . _:b212098310 . _:b84242 . _:b212098447 "2"^^ . _:b212098305 . _:b84243 . _:b212098446 "2"^^ . _:b212098343 . _:b84231 . _:b212098304 . _:b212098441 "2"^^ . _:b212098307 . _:b212098440 "2"^^ . _:b212098306 . _:b212098443 "2"^^ . _:b212098317 . _:b212098442 "2"^^ . _:b212098192 . _:b212098316 . _:b212098453 "2"^^ . _:b212098268 . _:b212098165 . _:b212098319 . _:b212098452 "2"^^ . _:b212098318 . _:b212098455 "2"^^ . . _:b212098313 . _:b212098454 "2"^^ . _:b212098407 . . _:b212098449 "2"^^ . _:b212098312 . . . _:b212098315 . _:b212098448 "2"^^ . _:b212098314 . _:b212098451 "2"^^ . _:b212098497 . _:b84234 . _:b212098325 . _:b212098450 "2"^^ . . . _:b212098324 . _:b212098461 "2"^^ . . . . _:b212098193 . . _:b212098461 . _:b212098327 . _:b212098460 "2"^^ . _:b212098231 . _:b212098173 . _:b212098326 . _:b212098463 "2"^^ . . _:b212098321 . _:b212098462 "2"^^ . . . . _:b212098320 . _:b212098457 "2"^^ . _:b212098435 . . _:b212098323 . _:b212098456 "2"^^ . . _:b212098322 . _:b212098459 "2"^^ . _:b212098502 . _:b212098333 . _:b212098458 "2"^^ . _:b212098346 . _:b212098332 . _:b212098469 "2"^^ . _:b84240 "These have been difficult to detect in vaccine trials with MAGE peptides (>>25<<), and difficult to interpret in trials with vaccines containing melanocyte differentiation antigens, since CTLs against these antigens can be generated in vitro from nonvaccinated melanoma patients as well as normal individuals (26, 27)." . _:b84228 . _:b212098332 . _:b212098335 . _:b212098468 "2"^^ . _:b84230 . . _:b212098221 . _:b212098334 . _:b212098471 "2"^^ . _:b212098329 . _:b212098470 "2"^^ . _:b212098242 . . _:b212098465 "2"^^ . _:b212098328 . . _:b212098331 . _:b212098464 "2"^^ . . _:b212098421 . _:b212098530 . _:b212098178 . _:b212098467 "2"^^ . _:b212098330 . . _:b212098527 . _:b212098341 . _:b212098466 "2"^^ . . _:b212098551 . . . _:b212098340 . _:b212098477 "2"^^ . _:b212098343 . _:b212098476 "2"^^ . . . _:b212098479 "2"^^ . . _:b212098342 . _:b212098322 . _:b84234 . _:b212098200 . _:b212098337 . _:b212098478 "2"^^ . _:b212098209 . _:b212098336 . _:b212098473 "2"^^ . _:b212098339 . _:b212098472 "2"^^ . . _:b212098338 . _:b212098475 "2"^^ . _:b212098202 . _:b212098197 . _:b212098474 "2"^^ . _:b212098349 . _:b212098348 . _:b212098485 "2"^^ . _:b212098351 . _:b212098484 "2"^^ . . _:b212098296 . _:b212098350 . _:b212098487 "2"^^ . . _:b212098345 . _:b212098486 "2"^^ . _:b212098391 . _:b212098344 . _:b84226 "Transfection of COS-7 cells was carried out as described (>>20<<). In brief, 2 \u00D7 104 COS-7 cells were transfected with 150 ng of plasmid pcDNA3.1(\u2212) containing NY-ESO-1 cDNA, and 150 ng of plasmid pcDNA1Amp containing HLA-A2.1 or HLA-A1 cDNA using the DEAE-dextran-chloroquine method." . _:b212098481 "2"^^ . . _:b212098347 . _:b212098480 "2"^^ . . _:b212098483 "2"^^ . _:b212098346 . _:b212098334 . _:b212098198 . . _:b212098482 "2"^^ . _:b212098357 . _:b212098314 . . _:b212098356 . _:b212098493 "2"^^ . _:b212098433 . _:b212098359 . _:b212098492 "2"^^ . _:b212098465 . _:b212098358 . _:b212098495 "2"^^ . . _:b212098369 . _:b212098353 . _:b212098494 "2"^^ . _:b212098434 . . _:b212098352 . _:b212098489 "2"^^ . . . _:b212098355 . _:b212098488 "2"^^ . _:b212098354 . _:b212098491 "2"^^ . _:b212098215 . . _:b212098345 . _:b212098365 . _:b212098490 "2"^^ . . _:b212098364 . _:b212098501 "2"^^ . . _:b212098321 . _:b212098367 . _:b212098500 "2"^^ . . _:b212098366 . _:b212098503 "2"^^ . . _:b212098361 . _:b212098502 "2"^^ . _:b212098360 . _:b212098497 "2"^^ . _:b212098363 . _:b212098496 "2"^^ . . _:b212098499 "2"^^ . _:b212098362 . . _:b212098498 "2"^^ . _:b212098373 . _:b84225 "Lytic activity of CTLs was tested in a 4-h chromium release assay as previously described (>>3<<). Peptide-specific CTL reactivity was determined on T2 cells labeled with 100 \u03BCCi of Na(51Cr)O4, 10 \u03BCg/ml of peptide, and 2.5 \u03BCg/ml of \u03B22-microglobulin." . _:b212098488 . _:b212098171 . _:b212098509 "2"^^ . _:b212098372 . . _:b212098219 . _:b212098508 "2"^^ . _:b212098375 . _:b212098175 . . . _:b212098511 "2"^^ . _:b212098374 . _:b212098315 . . _:b212098369 . _:b212098510 "2"^^ . _:b212098368 . _:b212098505 "2"^^ . _:b212098375 . _:b212098371 . _:b212098504 "2"^^ . _:b212098440 . . . _:b212098507 "2"^^ . _:b212098406 . _:b212098370 . _:b212098381 . _:b212098506 "2"^^ . . _:b212098380 . _:b212098517 "2"^^ . . _:b212098383 . _:b212098516 "2"^^ . _:b212098382 . _:b212098519 "2"^^ . . _:b212098377 . _:b212098518 "2"^^ . . _:b212098513 "2"^^ . _:b212098376 . . . _:b212098379 . _:b212098512 "2"^^ . . _:b212098515 "2"^^ . _:b212098378 . . . _:b212098389 . _:b84231 "Evidence for a specific humoral response to human cancer came from serological analysis of cell surface reactivity of sera from cancer patients for autologous cancer cells, an approach called autologous typing (>>4<<). However, with only a few exceptions, this approach did not allow for the structural definition of the antigenic target. An autologous typing system also provided the first evidence for the development of CTLs with specificity for human" . _:b212098514 "2"^^ . _:b212098388 . _:b212098525 "2"^^ . _:b212098391 . _:b212098524 "2"^^ . _:b212098335 . _:b212098390 . _:b212098527 "2"^^ . . _:b212098255 . _:b212098526 "2"^^ . _:b212098385 . _:b212098289 . _:b212098384 . _:b212098521 "2"^^ . . _:b212098485 . . _:b212098520 "2"^^ . _:b212098387 . _:b84238 . _:b212098386 . _:b212098523 "2"^^ . _:b212098397 . _:b212098522 "2"^^ . _:b212098396 . _:b212098533 "2"^^ . _:b212098399 . _:b212098532 "2"^^ . _:b212098398 . _:b212098535 "2"^^ . . _:b84236 . _:b212098261 . _:b212098534 "2"^^ . _:b212098393 . . _:b212098392 . _:b212098529 "2"^^ . _:b212098395 . _:b212098528 "2"^^ . _:b212098394 . _:b212098531 "2"^^ . . _:b212098546 . _:b212098409 . _:b212098405 . _:b212098530 "2"^^ . . . _:b212098541 "2"^^ . _:b212098404 . _:b212098237 . _:b212098407 . _:b212098540 "2"^^ . _:b212098406 . _:b212098543 "2"^^ . . _:b212098401 . _:b212098542 "2"^^ . _:b212098467 . _:b212098339 . _:b212098400 . _:b212098537 "2"^^ . . _:b212098536 "2"^^ . _:b212098403 . _:b212098402 . _:b212098539 "2"^^ . _:b212098158 . . _:b212098538 "2"^^ . _:b212098413 . . _:b212098412 . _:b212098549 "2"^^ . _:b212098416 . . . _:b212098415 . _:b212098548 "2"^^ . _:b212098491 . _:b212098349 . _:b212098551 "2"^^ . _:b212098414 . _:b84236 "Using specific antitumor CTLs as probes, a number of CTL targets have been cloned on the basis of MHC class I\u2013restricted recognition (1, >>6<<). However, this approach involves cultured cancer cell lines and stable CTL lines from the same patient, two requirements that cannot easily be met with many tumor types. With the demonstration that genes coding for CTL-recognized tumor" . _:b212098409 . _:b212098550 "2"^^ . _:b212098338 . _:b212098408 . _:b212098545 "2"^^ . . _:b212098411 . _:b212098544 "2"^^ . _:b212098410 . _:b212098547 "2"^^ . _:b212098446 . . _:b212098421 . _:b212098546 "2"^^ . _:b212098495 . _:b212098420 . _:b212098557 "2"^^ . _:b212098420 . _:b212098423 . _:b212098556 "2"^^ . _:b212098422 . _:b212098559 "2"^^ . . . . _:b212098558 "2"^^ . _:b212098417 . _:b212098266 . _:b212098442 . . _:b212098416 . _:b212098553 "2"^^ . . _:b212098419 . _:b212098552 "2"^^ . _:b212098418 . _:b212098555 "2"^^ . _:b212098532 . . _:b212098554 "2"^^ . _:b212098429 . _:b212098274 . . _:b212098428 . . . _:b212098431 . _:b212098396 . _:b212098389 . _:b212098430 . . _:b212098425 . _:b212098424 . . . . _:b212098236 . _:b212098427 . . . _:b212098426 . . _:b212098437 . . _:b212098204 . _:b212098436 . _:b212098167 . _:b212098439 . . _:b212098438 . _:b84241 . _:b212098433 . _:b212098542 . . _:b212098432 . _:b212098258 . _:b212098480 . _:b212098435 . _:b212098365 . _:b212098434 . _:b212098535 . _:b212098279 . _:b212098445 . . _:b212098444 . _:b212098357 . _:b212098447 . . _:b212098446 . _:b212098293 . _:b212098441 . _:b212098337 . _:b212098440 . . _:b212098452 . _:b212098547 . . . _:b212098443 . _:b212098318 . . _:b212098311 . _:b212098442 . . . _:b212098292 . _:b84239 . _:b212098453 . . _:b212098452 . . _:b212098455 . _:b212098454 . _:b84234 . _:b212098163 . _:b212098449 . . _:b212098448 . _:b212098328 . _:b84229 . . _:b212098451 . . . . . _:b212098450 . _:b212098461 . _:b212098503 . _:b212098460 . _:b212098554 . . . _:b212098463 . _:b212098187 . _:b212098462 . _:b212098401 . _:b212098457 . _:b212098308 . _:b212098169 . _:b212098456 . _:b84232 . _:b212098459 . . _:b212098458 . . _:b212098189 . _:b212098521 . _:b212098382 . _:b212098469 . _:b212098468 . _:b212098457 . _:b212098513 . _:b212098471 . _:b212098428 . _:b212098470 . _:b212098465 . . . . _:b84242 . _:b212098464 . _:b212098467 . _:b84225 . _:b212098223 . _:b212098267 . _:b212098466 . . _:b212098477 . . . _:b212098476 . . _:b212098479 . _:b212098478 . _:b84223 "Serum antibody responses against the full-length recombinant NY-ESO-1 protein, a lysate of NY-ESO-1\u2013transfected COS-7 cells, and a lysate of the autologous tumor cell line NW-MEL-38 were tested by standard Western blot analysis (>>18<<). In brief, 1 \u03BCg of NY-ESO-1 protein or lysates of 2 \u00D7 104 NY-ESO-1\u2013transfected COS-7 cells (containing 7.7 \u03BCg of protein) or of 5 \u00D7 103 tumor cells were diluted in SDS, and electrophoresed on a 15% SDS gel." . . . _:b212098473 . _:b212098472 . . _:b212098475 . _:b212098474 . _:b212098485 . _:b212098470 . _:b212098484 . _:b212098487 . . _:b212098486 . . . _:b212098481 . . . _:b212098480 . . _:b212098483 . _:b212098177 . _:b212098443 . . _:b212098482 . _:b212098493 . _:b212098190 . _:b212098492 . . _:b212098229 . _:b212098495 . . _:b212098248 . _:b212098494 . _:b84233 . . . _:b212098489 . . _:b212098488 . _:b212098491 . . _:b212098490 . _:b212098323 . _:b212098501 . _:b212098270 . . _:b212098388 . _:b212098500 . _:b212098195 . _:b84232 "An autologous typing system also provided the first evidence for the development of CTLs with specificity for human melanoma cells (>>3<<, 17, 21\u201324)." . _:b212098503 . _:b212098523 . _:b212098502 . _:b212098196 . _:b212098182 . _:b212098276 . _:b212098344 . _:b212098497 . . _:b212098496 . _:b212098499 . . _:b212098498 . _:b212098500 . _:b212098509 . _:b212098367 . . _:b84227 "Transfectants were tested for their ability to stimulate the production of TNF-\u03B1 by NW38-IVS-1, as described (>>20<<). In brief, 2,500 CTLs in 100 \u03BCl RPMI supplemented with 10% human serum and 25 U/ml recombinant human IL-2 were added to microwells containing COS-7 transfectants." . _:b212098508 . _:b212098511 . _:b212098395 . _:b212098510 . _:b212098348 . _:b212098505 . _:b212098504 . . _:b212098507 . . _:b212098506 . _:b212098517 . _:b212098516 . . _:b212098519 . . _:b212098305 . _:b212098518 . _:b212098238 . _:b212098217 . . _:b212098513 . _:b84233 "An autologous typing system also provided the first evidence for the development of CTLs with specificity for human melanoma cells (3, >>17<<, 21\u201324). Using specific antitumor CTLs as probes, a number of CTL targets have been cloned on the basis of MHC class I\u2013restricted recognition (1, 6). However, this approach involves cultured cancer cell lines and stable CTL lines from the" . _:b212098512 . _:b84234 . _:b212098475 . _:b212098515 . . _:b212098448 . _:b212098514 . _:b212098525 . _:b212098341 . _:b212098524 . _:b212098450 . _:b212098527 . _:b212098526 . _:b212098521 . _:b212098520 . _:b212098447 . . . _:b212098523 . _:b212098225 . _:b212098522 . _:b212098543 . _:b212098533 .