_:b547271304 . _:b547271327 "3"^^ . _:b547271456 . . _:b547271321 . _:b547271307 . _:b547271326 "3"^^ . . _:b547271321 "3"^^ . _:b547271306 . . _:b547271317 . _:b547271320 "3"^^ . . . _:b547271323 "3"^^ . _:b547271316 . . _:b547271287 . _:b547271322 "3"^^ . _:b547271319 . . _:b547271318 . _:b547271333 "3"^^ . _:b547271313 . _:b547271332 "3"^^ . _:b547271294 . _:b547271312 . _:b547271335 "3"^^ . _:b547271315 . _:b547271430 . _:b547271334 "3"^^ . _:b547271084 . _:b547271314 . _:b547271366 . _:b547271329 "3"^^ . . _:b547271693 . _:b547271819 . . _:b547271965 . _:b547271328 "3"^^ . _:b547271325 . . . _:b547271324 . _:b547271413 . _:b547271378 . _:b547271331 "3"^^ . _:b547271327 . _:b547271330 "3"^^ . . . _:b547271352 . . _:b547271341 "3"^^ . _:b547271326 . . . . _:b547271321 . _:b547271340 "3"^^ . . . _:b29539917 "RUNX2 gene expression increased with the duration of culture on TCPS (1 to 7 days; DT1 to DT7; Fig. 1b). These data suggested to us that the extent of exposure to a culture environment, or mechanical dose, alone can bias hMSC behavior.>>17<< That is, hMSCs store information from physical environments and this mechanical history influences future fate." . _:b547271320 . _:b547271343 "3"^^ . . . _:b547271490 . _:b547271323 . _:b547271379 . _:b547271342 "3"^^ . _:b547271322 . _:b547271337 "3"^^ . _:b547271957 . _:b547271333 . _:b547271705 . _:b547271336 "3"^^ . _:b547271371 . . _:b547271332 . _:b547271339 "3"^^ . . _:b547271335 . _:b547271338 "3"^^ . _:b547271334 . _:b547271349 "3"^^ . _:b547271041 . . . _:b547271348 "3"^^ . _:b547271329 . . _:b547271054 . _:b547271351 "2"^^ . _:b547271328 . _:b547271636 . . _:b547271350 "2"^^ . _:b547271331 . _:b547271330 . _:b547271345 "3"^^ . _:b547271341 . _:b547271344 "3"^^ . . _:b547271340 . _:b547271347 "3"^^ . _:b547271343 . _:b547271346 "3"^^ . _:b547271954 . . _:b547271357 "2"^^ . _:b547271342 . _:b547271356 "2"^^ . _:b547271337 . . _:b547271359 "2"^^ . . _:b547271336 . _:b547271358 "2"^^ . . _:b547271339 . . _:b547271353 "2"^^ . _:b547271338 . _:b547271786 . _:b547271352 "2"^^ . _:b547271583 . . _:b547271349 . . _:b547271355 "2"^^ . _:b547271348 . _:b547271840 . _:b547271206 . _:b547271855 . _:b547271123 . _:b547271354 "2"^^ . _:b547271351 . _:b547271365 "2"^^ . _:b547271350 . . _:b547271364 "2"^^ . . _:b547271345 . _:b547271869 . _:b547271933 . _:b547271367 "2"^^ . _:b547271344 . _:b547271362 . . _:b547271366 "2"^^ . . _:b547271347 . _:b547271573 . _:b547271472 . _:b547271489 . _:b547271361 "2"^^ . _:b547271742 . _:b547271346 . _:b547271360 "2"^^ . . _:b547271824 . _:b547271787 . _:b547271357 . _:b547270995 . _:b547271223 . _:b547271363 "2"^^ . _:b547271356 . . _:b547271362 "2"^^ . _:b547271359 . _:b547271691 . . _:b547271373 "2"^^ . _:b547271358 . . _:b547271468 . . _:b547271372 "2"^^ . _:b547271353 . _:b547271115 . _:b547271467 . _:b547271375 "2"^^ . _:b547271116 . _:b547271352 . _:b547271374 "2"^^ . _:b547271355 . _:b29539903 . _:b547271369 "2"^^ . _:b547271354 . _:b547271886 . . . _:b547271368 "2"^^ . _:b547271365 . _:b547271371 "2"^^ . _:b547271595 . . _:b547271698 . _:b547271364 . _:b547271370 "2"^^ . _:b547271367 . _:b547271794 . _:b547271381 "2"^^ . _:b29539927 "ir)reversible effects of mechanical dosing" . _:b29539908 . _:b547271366 . _:b547271380 "2"^^ . _:b547271361 . _:b29539909 . . . _:b547271383 "2"^^ . _:b547271360 . _:b547271949 . _:b29539910 . _:b547271382 "2"^^ . . _:b29539911 . _:b547271363 . _:b547271377 "2"^^ . _:b547271362 . _:b29539904 . _:b547271376 "2"^^ . _:b547271373 . _:b29539905 . _:b547271379 "2"^^ . _:b547271372 . _:b29539906 . . _:b547271254 . _:b547271784 . _:b547271378 "2"^^ . _:b547271375 . _:b29539907 . _:b547271389 "2"^^ . _:b547271374 . _:b29539916 . _:b547271642 . . _:b547271388 "2"^^ . . _:b29539917 . _:b547271369 . _:b547271391 "2"^^ . _:b547271368 . _:b29539918 . . _:b547271554 . _:b547271390 "2"^^ . _:b547271371 . _:b29539919 . . _:b547271385 "2"^^ . _:b547271370 . _:b29539912 . _:b547271384 "2"^^ . _:b547271381 . _:b29539913 . _:b547271939 . _:b547271387 "2"^^ . _:b547271380 . _:b29539914 . _:b547271599 . _:b547271386 "2"^^ . _:b547271383 . _:b29539915 . . . _:b547271709 . _:b547271397 "2"^^ . _:b547271382 . _:b29539924 . _:b547271396 "2"^^ . _:b547271377 . _:b29539925 . . _:b547271399 "2"^^ . . _:b29539926 . _:b547271376 . _:b547271398 "2"^^ . _:b547271379 . _:b29539927 . . _:b547271393 "2"^^ . . _:b547271378 . _:b29539920 . _:b547271392 "2"^^ . _:b547271389 . _:b29539921 . _:b547271412 . . _:b547271395 "2"^^ . _:b547271388 . _:b29539922 . . _:b547271394 "2"^^ . _:b547271111 . _:b547271391 . _:b29539923 . . _:b547271174 . _:b547271405 "2"^^ . _:b547271390 . _:b547271867 . _:b547271375 . _:b547271404 "2"^^ . _:b547271385 . _:b547271143 . _:b547271407 "2"^^ . _:b547271384 . _:b547271482 . _:b547271406 "2"^^ . _:b547271246 . _:b547271387 . . _:b547271401 "2"^^ . _:b547271386 . _:b29539928 . _:b547271400 "2"^^ . _:b547271981 . _:b547271397 . _:b29539929 . . _:b547271403 "2"^^ . _:b547271396 . _:b29539930 . _:b547271402 "2"^^ . . _:b547271420 . _:b547271399 . . _:b547271413 "2"^^ . _:b547271398 . . . _:b547271412 "2"^^ . _:b547271393 . . . _:b547271415 "2"^^ . . _:b547271392 . _:b547271631 . _:b547271414 "2"^^ . . _:b547271395 . . _:b547271409 "2"^^ . _:b547271026 . _:b547271394 . _:b547271408 "2"^^ . _:b547271405 . _:b547271388 . _:b547271411 "2"^^ . . _:b547271404 . . _:b547271410 "2"^^ . _:b547271407 . _:b547271158 . _:b547271421 "2"^^ . _:b547271475 . _:b547271427 . . _:b547271406 . _:b547271747 . _:b547271420 "2"^^ . _:b547271401 . . . _:b547271423 "2"^^ . _:b547271400 . . . . _:b547271422 "2"^^ . . _:b547271462 . _:b547271403 . _:b547271417 "2"^^ . _:b547271402 . _:b547271416 "2"^^ . _:b547271413 . _:b547271593 . _:b547271419 "2"^^ . _:b547271412 . _:b547271418 "2"^^ . _:b547271415 . . _:b547271429 "2"^^ . _:b547271414 . _:b547271774 . _:b547271428 "2"^^ . _:b547271409 . _:b547271504 . . . . _:b547271431 "2"^^ . . . _:b547271408 . _:b547271224 . _:b547271230 . _:b547271335 . _:b547271094 . _:b547271430 "2"^^ . . _:b547271411 . . _:b547271425 "2"^^ . . _:b547271410 . . _:b547271424 "2"^^ . _:b547271421 . . _:b547271293 . _:b547271427 "2"^^ . _:b547271420 . _:b547271880 . _:b547271906 . . _:b547271426 "2"^^ . . _:b547271423 . _:b547271477 . _:b547271437 "2"^^ . _:b547271422 . _:b547271436 "2"^^ . _:b547271417 . _:b547271550 . . _:b547271439 "2"^^ . _:b547271416 . . _:b547271438 "2"^^ . _:b547271419 . . _:b547271433 "2"^^ . _:b547271418 . . _:b547271432 "2"^^ . _:b547271435 "2"^^ . _:b547271485 . . . _:b547271434 "2"^^ . _:b547271445 "2"^^ . . _:b547271739 . _:b547271444 "2"^^ . . _:b547271447 "2"^^ . _:b547271506 . _:b547271446 "2"^^ . . . _:b547271441 "2"^^ . _:b547271440 "2"^^ . _:b547271172 . _:b547271885 . _:b547271443 "2"^^ . . _:b547271442 "2"^^ . . . _:b547271453 "2"^^ . . _:b547271452 "2"^^ . _:b547271455 "2"^^ . . _:b547271454 "2"^^ . . _:b547271449 "2"^^ . _:b547271442 . _:b547271448 "2"^^ . . _:b547271451 "2"^^ . _:b547271558 . _:b547271326 . _:b547271450 "2"^^ . _:b547271895 . _:b547271461 "2"^^ . . _:b547271460 "2"^^ . . _:b547271845 . _:b547271463 "2"^^ . . _:b547271079 . _:b547271462 "2"^^ . . _:b547271534 . _:b547271457 "2"^^ . _:b547271875 . . _:b547271456 "2"^^ . _:b547271344 . _:b547271459 "2"^^ . _:b547271458 "2"^^ . . _:b547271469 "2"^^ . _:b547271468 "2"^^ . _:b547271471 "2"^^ . . . _:b547271470 "2"^^ . _:b547271610 . _:b547271465 "2"^^ . . _:b547271464 "2"^^ . . _:b547271467 "2"^^ . . _:b547271448 . _:b547271466 "2"^^ . . _:b547271477 "2"^^ . _:b547271476 "2"^^ . _:b547271470 . . _:b547271479 "2"^^ . . _:b547271402 . _:b547271478 "2"^^ . . . . _:b547271473 "2"^^ . _:b547271472 "2"^^ . . . _:b547271475 "2"^^ . _:b547271403 . _:b547271474 "2"^^ . _:b547271937 . . _:b547271680 . _:b547271485 "2"^^ . _:b29539904 "Polyethylene glycol di-photodegradable acrylate (PEGdiPDA) was synthesized and characterized as previously described.>>18<<,19 The adhesive peptide OOGRGDSG (diethylene glycol-diethylene glycol-glycine-arginine-glycine-aspartic acid-serine-glycine) was synthesized (Protein Technologies Tribute peptide synthesizer) through Fmoc solid-phase methodology and HATU" . _:b547271484 "2"^^ . _:b547271045 . _:b547271616 . _:b547271142 . _:b547271487 "2"^^ . _:b547271952 . . _:b547271921 . . _:b547271486 "2"^^ . . _:b547271502 . _:b547271481 "2"^^ . . _:b547271480 "2"^^ . _:b547271483 "2"^^ . _:b547271200 . _:b547271482 "2"^^ . _:b547271923 . . . _:b547271493 "2"^^ . _:b547271154 . _:b547271391 . _:b547271492 "2"^^ . . _:b547271796 . _:b547271495 "2"^^ . _:b547271389 . . _:b547271494 "2"^^ . . . . _:b547271489 "2"^^ . . _:b547271653 . . _:b547271644 . . _:b547271488 "2"^^ . . _:b547271491 "2"^^ . _:b547271905 . _:b547271727 . . _:b547271490 "2"^^ . . _:b547271501 "2"^^ . _:b547271125 . _:b547271532 . _:b547271463 . _:b547271500 "2"^^ . _:b547271204 . _:b547271425 . _:b547271503 "2"^^ . . _:b547271842 . _:b547271502 "2"^^ . _:b547271525 . _:b547271497 "2"^^ . . _:b547271496 "2"^^ . _:b547271591 . _:b29539903 "methods" . . . _:b547271499 "2"^^ . . _:b547271683 . . . _:b547271270 . _:b547271498 "2"^^ . _:b547271622 . _:b547271881 . . _:b547271509 "2"^^ . . . _:b547271703 . _:b547271405 . _:b547271508 "2"^^ . . _:b547271511 "2"^^ . _:b547271300 . _:b547271510 "2"^^ . . _:b547271505 "2"^^ . _:b547271449 . _:b547271504 "2"^^ . _:b547270989 . _:b547271507 "2"^^ . _:b547270988 . _:b547271506 "2"^^ . _:b547270991 . _:b547271517 "2"^^ . . _:b547270990 . . _:b547271516 "2"^^ . . _:b547271519 "2"^^ . . _:b547271458 . _:b547271518 "2"^^ . _:b547271513 "2"^^ . . _:b547271512 "2"^^ . _:b547270997 . _:b547271107 . . _:b547271515 "2"^^ . _:b547270996 . . . _:b547271514 "2"^^ . _:b547270999 . . . _:b547271525 "2"^^ . _:b547270998 . _:b547271524 "2"^^ . _:b547270993 . _:b547271862 . _:b547271724 . . _:b547271527 "2"^^ . _:b547270992 . . _:b547271065 . . _:b547271526 "2"^^ . _:b547270995 . _:b547271076 . _:b547271521 "2"^^ . _:b29539913 "changes induced by constitutive YAP activation mediated genetically, epigenetically, or structurally?30 There is strong evidence that YAP/TAZ mechanotransduction depends directly on the organization of the actin cytoskeleton.>>15<<,16 In preliminary experiments in this setting, the effects of mechanical dosing persisted even after disruption of the actin cytoskeleton with latrunculin A (Supplementary Fig." . _:b547270994 . _:b547271520 "2"^^ . _:b547271005 . _:b547271523 "2"^^ . _:b29539929 "ex vivo (e.g., passage number and substrate modulus).17 Experiments on cell differentiation, cell function, or cell transplantation, in particular those employing multipotent stem cells, may be confounded by unintended mechanical dosing.>>14<< Additionally, stem cells are differentiated, in standard protocols, by delivering specific doses of chemical factors during culture." . _:b547271004 . . _:b547271522 "2"^^ . _:b547271007 . _:b547271533 "2"^^ . _:b547271006 . _:b547271390 . _:b547271532 "2"^^ . _:b547271722 . _:b547271001 . _:b547271030 . _:b547271535 "2"^^ . _:b547271000 . . . _:b547271534 "2"^^ . _:b547271003 . _:b547271529 "2"^^ . _:b547271002 . _:b547271528 "2"^^ . _:b547271013 . _:b547271101 . _:b547271531 "2"^^ . _:b547271012 . _:b547271515 . _:b547271766 . _:b547271530 "2"^^ . _:b547271189 . _:b547271095 . _:b547271015 . _:b547271541 "2"^^ . _:b547271014 . _:b547271540 "2"^^ . _:b547271009 . _:b547271543 "2"^^ . . _:b547271008 . _:b547271542 "2"^^ . _:b547271011 . _:b547271836 . _:b547271284 . _:b547271537 "2"^^ . _:b547271010 . _:b547271382 . _:b547271536 "2"^^ . _:b547271852 . _:b547271021 . . _:b547271539 "2"^^ . _:b547271020 . _:b547271538 "2"^^ . _:b547271023 . . . _:b547271549 "2"^^ . _:b547271022 . _:b547271392 . . _:b547271548 "2"^^ . _:b547271017 . . _:b547271551 "2"^^ . _:b547271016 . . _:b547271550 "2"^^ . _:b547271019 . . _:b547271545 "2"^^ . _:b547271883 . _:b547271018 . _:b547271544 "2"^^ . _:b547271563 . _:b547271029 . _:b547271501 . . _:b547271547 "2"^^ . _:b547271028 . _:b547271935 . . _:b547271546 "2"^^ . _:b547271031 . _:b547271150 . _:b547271424 . . _:b547271557 "2"^^ . _:b547271030 . . _:b547271556 "2"^^ . _:b547271025 . _:b547271250 . _:b547271948 . _:b547271198 . _:b547271559 "2"^^ . _:b547271024 . . . _:b547271558 "2"^^ . _:b547271027 . . _:b547271553 "2"^^ . _:b547271026 . . . _:b547271552 "2"^^ . _:b547271037 . _:b547271469 . _:b547271555 "2"^^ . _:b547271036 . _:b547271313 . _:b547271037 . _:b547271554 "2"^^ . _:b547271039 . . _:b547271565 "2"^^ . _:b547271512 . _:b547271038 . _:b547271615 . . _:b547271564 "2"^^ . _:b547271033 . _:b547271567 "2"^^ . _:b547271770 . _:b547271032 . _:b547271330 . . _:b547271052 . _:b547271566 "2"^^ . _:b547271035 . _:b547271934 . . _:b547271561 "2"^^ . _:b547271034 . . _:b547271874 . _:b547271560 "2"^^ . _:b547271045 . _:b547271563 "2"^^ . _:b547271044 . _:b547271562 "2"^^ . _:b547271047 . . _:b547271573 "2"^^ . _:b547271046 . _:b547271943 . . . _:b547271572 "2"^^ . _:b547271041 . _:b547271518 . _:b547271572 . _:b547271575 "2"^^ . . _:b29539921 . _:b547271040 . . _:b547271574 "2"^^ . . _:b547271043 . _:b547271569 "2"^^ . _:b547271042 . . _:b547271568 "2"^^ . _:b547271053 . _:b29539918 . _:b547271144 . _:b547271118 . _:b547271930 . _:b547271571 "2"^^ . _:b547271052 . . _:b547271570 "2"^^ . _:b547271055 . _:b547271015 . _:b547271581 "2"^^ . _:b547271054 . . _:b547271580 "2"^^ . _:b547271049 . _:b547271289 . _:b547271161 . . _:b547271583 "2"^^ . _:b547271048 . _:b29539913 . _:b547271277 . . _:b547271582 "2"^^ . _:b547271050 . _:b547271051 . . . _:b547271577 "2"^^ . _:b547271050 . _:b547271273 . . . _:b547271576 "2"^^ . _:b547271061 . _:b547271625 . _:b547271579 "2"^^ . _:b547271748 . _:b547271060 . _:b547271673 . _:b547271578 "2"^^ . _:b547271063 . . . _:b547271589 "2"^^ . _:b547271062 . . . _:b547271588 "2"^^ . _:b547271057 . . _:b547271591 "2"^^ . _:b547271056 . . . _:b547271590 "2"^^ . _:b547271059 . _:b547271333 . _:b547271621 . _:b547271585 "2"^^ . _:b547271058 . . _:b547271849 . . _:b547271843 . _:b547271584 "2"^^ . _:b547271069 . . . . _:b29539929 . _:b547271587 "2"^^ . _:b547271068 . _:b547271850 . _:b547271789 . _:b547271586 "2"^^ . _:b547271017 . _:b547271071 . _:b547271597 "2"^^ . _:b547271070 . . _:b547271132 . _:b547271596 "2"^^ . _:b547271065 . _:b547271746 . _:b547271599 "2"^^ . . _:b547271799 . _:b547271064 . _:b547271039 . . _:b547271598 "2"^^ . _:b547271067 . . _:b547271593 "2"^^ . _:b547271066 . . _:b547271473 . _:b547271592 "2"^^ . . _:b547271077 . . . _:b547271595 "2"^^ . _:b547271076 . _:b547271516 . _:b547271594 "2"^^ . _:b547271079 . . _:b547271605 "2"^^ . . _:b547271078 . _:b547271604 "2"^^ . _:b547271847 . _:b547271689 . _:b547271073 . _:b547271790 . _:b547271607 "2"^^ . _:b547271072 . _:b547271271 . _:b547271606 "2"^^ . _:b547271075 . _:b547271601 "2"^^ . _:b547271074 . _:b547271820 . . . _:b547271453 . _:b547271600 "2"^^ . _:b547271185 . _:b547271085 . . _:b547271228 . . _:b547271603 "2"^^ . _:b547271084 . _:b547271602 "2"^^ . _:b547271087 . _:b547271975 . _:b547271613 "2"^^ . _:b547271961 . _:b547271086 . _:b547271660 . _:b547271137 . . . _:b547271612 "2"^^ . _:b547271931 . _:b547271081 . . . _:b547271615 "2"^^ . _:b547271080 . _:b547271614 "2"^^ . _:b547271083 . . _:b547271609 "2"^^ . _:b547271082 . . _:b547271608 "2"^^ . . _:b547271093 . _:b547271611 "2"^^ . _:b547271092 . . _:b547271777 . _:b547271773 . _:b547271180 . . . _:b547271610 "2"^^ . . _:b547271095 . _:b547271280 . _:b547271708 . _:b547271621 "2"^^ . . _:b547271094 . . _:b547271620 "2"^^ . . _:b547271089 . _:b547271623 "2"^^ . _:b547271088 . _:b547271093 . _:b547271622 "2"^^ . _:b547271091 . . _:b547271617 "2"^^ . _:b547271090 . _:b29539924 . . _:b547271616 "2"^^ . _:b547271073 . _:b547271101 . . _:b547271619 "2"^^ . _:b547271100 . _:b547271618 "2"^^ . _:b547271103 . . . _:b547271629 "2"^^ . . _:b547271102 . _:b547271628 "2"^^ . . . _:b547271097 . _:b547271631 "2"^^ . _:b547271096 . _:b547271711 . _:b547271630 "2"^^ . _:b547271099 . _:b547271625 "2"^^ . _:b547271098 . _:b547271816 . _:b547271740 . _:b29539924 "substrate in situ and in the presence of cells. To accomplish this, we synthesized dynamically tunable, photodegradable hydrogels based on a poly(ethylene glycol) di-photodegradable acrylate (PEGdiPDA) crosslinker (Fig. 2a).>>18<<-20 PEGdiPDA photodegradable hydrogels enable the user to tune material properties exogenously with precise control21-23 and have been exploited to investigate the cellular response to dynamic alterations in substrate mechanics." . . _:b547271624 "2"^^ . _:b547271109 . . . _:b547271627 "2"^^ . _:b547271108 . _:b547271262 . _:b547271626 "2"^^ . _:b547271111 . . . . _:b547271637 "2"^^ . _:b547271110 . _:b547271636 "2"^^ . _:b547271734 . _:b547271105 . . . _:b547271639 "2"^^ . . _:b547271104 . . _:b547271638 "2"^^ . _:b547271107 . _:b547271568 . . . _:b547271633 "2"^^ . _:b547271106 . . . . _:b547271632 "2"^^ . _:b547271117 . . _:b547271190 . . _:b547271635 "2"^^ . _:b547271116 . . _:b547271055 . _:b547271634 "2"^^ . . _:b29539905 . _:b547271119 . _:b547271659 . _:b547271645 "2"^^ . _:b547271083 . _:b547271478 . _:b547271118 . _:b547271644 "2"^^ . _:b547271113 . . _:b547271647 "2"^^ . _:b547271809 . _:b547271112 . . _:b547271646 "2"^^ . _:b547271364 . _:b547271115 . . _:b547271307 . _:b547271641 "2"^^ . _:b547271114 . . _:b547271044 . . _:b547271640 "2"^^ . _:b547271125 . . _:b29539916 "mechanical memory and dosing" . . _:b547271643 "2"^^ . _:b547271124 . _:b547271134 . _:b547271642 "2"^^ . . . _:b547271127 . _:b547271653 "2"^^ . _:b547271126 . . _:b547271439 . _:b547271652 "2"^^ . . _:b547271121 . . _:b547271623 . _:b547271655 "2"^^ . _:b547271120 . . . _:b547271654 "2"^^ . _:b547271123 . _:b547271582 . . _:b29539908 "The preparation of PEGdiPDA, photodegradable hydrogels was adapted from previously described protocols. 20,>>25<< Briefly, PEGdiPDA was co-polymerized with poly(ethylene glycol) monoacrylate (PEGA; Mn ~ 400 Da; Monomer-Polymer and Dajac Laboratories, Inc." . _:b547271649 "2"^^ . _:b547271122 . _:b547271109 . . . _:b547271648 "2"^^ . _:b547271133 . _:b547271522 . _:b547271651 "2"^^ . _:b547271132 . _:b547271569 . _:b547271650 "2"^^ . _:b547271135 . _:b547271661 "2"^^ . _:b547271134 . _:b547271692 . _:b547271941 . _:b547271029 . _:b547271771 . _:b547271660 "2"^^ . _:b547271129 . _:b547271940 . _:b547271663 "2"^^ . _:b547271128 . _:b547271556 . _:b547271943 . _:b547271662 "2"^^ . _:b29539905 "Polyethylene glycol di-photodegradable acrylate (PEGdiPDA) was synthesized and characterized as previously described.18,>>19<< The adhesive peptide OOGRGDSG (diethylene glycol-diethylene glycol-glycine-arginine-glycine-aspartic acid-serine-glycine) was synthesized (Protein Technologies Tribute peptide synthesizer) through Fmoc solid-phase methodology and HATU" . _:b547271131 . _:b547271942 . _:b547270989 . _:b547271657 "2"^^ . _:b547271130 . _:b547271937 . _:b547270994 . _:b547271656 "2"^^ . _:b547271141 . _:b547271519 . _:b547271936 . _:b547271659 "2"^^ . _:b547271140 . _:b547271939 . _:b547271658 "2"^^ . _:b547271143 . _:b547271938 . _:b547271608 . _:b547271669 "2"^^ . _:b547271142 . _:b547271736 . _:b547271949 . _:b547271668 "2"^^ . _:b547271137 . _:b547271858 . _:b547271710 . _:b547271948 . _:b547271136 . _:b547271671 "2"^^ . _:b547271394 . _:b547271136 . . _:b547271951 . _:b547271600 . _:b547271194 . _:b547271670 "2"^^ . _:b547271139 . _:b547271950 . _:b547271665 "2"^^ . _:b547271138 . _:b547271945 . _:b547271664 "2"^^ . _:b547271149 . _:b547271919 . _:b547271305 . _:b547271944 . _:b547271208 . _:b547271667 "2"^^ . _:b547271148 . . _:b547271947 . _:b547271666 "2"^^ . _:b547271151 . . _:b547271946 . _:b547271677 "2"^^ . _:b547271150 . . _:b547271957 . _:b547271676 "2"^^ . _:b547271145 . _:b547271956 . _:b547271046 . . _:b547271679 "2"^^ . _:b547271144 . _:b547271801 . . _:b547271959 . . . _:b547271678 "2"^^ . _:b547271103 . _:b547271147 . . . _:b547271958 . _:b547271673 "2"^^ . _:b547271146 . _:b547271953 . _:b547271873 . _:b547271672 "2"^^ . _:b547271157 . _:b547271952 . . _:b547271675 "2"^^ . . _:b547271156 . _:b547271955 . _:b547271958 . _:b547271674 "2"^^ . . _:b547271159 . _:b547271954 . _:b547271145 . _:b547271248 . _:b547271158 . _:b547271337 . . _:b547271965 . _:b547271153 . _:b547271964 . _:b547271152 . _:b547271598 . . _:b547271967 . _:b547271155 . _:b547271681 . _:b547271966 . _:b547271154 . _:b547271580 . _:b547271961 . _:b547271165 . _:b547271112 . _:b547271960 . . _:b547271164 . . . _:b547271963 . _:b547271901 . _:b547271167 . _:b547271962 . . _:b547271166 . _:b547271175 . _:b547271973 . . _:b547271161 . _:b547271213 . _:b547271972 . _:b547271941 . _:b547271016 . _:b547271160 . _:b547271975 . . _:b547271163 . _:b547271974 . _:b547271730 . . . _:b547271162 . _:b547271638 . _:b547271969 . . _:b547271641 . . _:b547271571 . _:b547271968 . _:b547271971 . _:b547271089 . _:b547271970 . . . _:b547271981 . _:b547271980 . _:b547271183 . . _:b547271977 . _:b547271461 . _:b547271976 . . . _:b547271979 . _:b547271978 . . . _:b547271718 . . _:b547271916 . _:b547270992 . . . . _:b547271760 . _:b547271179 . _:b547271386 . _:b29539926 . _:b547271176 . _:b547271444 . _:b29539924 . . . . _:b547271893 . _:b547271162 . . . . _:b547271303 . . _:b547271426 . _:b547271864 . . . _:b547271821 . _:b547271429 . _:b547271778 . _:b547271487 . _:b547271783 . _:b547271471 . _:b29539912 "exposure to microenvironmental cues may lead to reversible and irreversible (long-term) effects on stem cells. Are the irreversible changes induced by constitutive YAP activation mediated genetically, epigenetically, or structurally?>>30<< There is strong evidence that YAP/TAZ mechanotransduction depends directly on the organization of the actin cytoskeleton." . . . . . _:b547271767 . _:b547271498 . _:b547271034 . _:b547271363 . _:b29539904 . _:b547271594 . _:b547271459 . _:b547271635 . _:b547271182 . _:b547271075 . _:b547271370 . _:b547271121 . . . _:b547271918 . . _:b547271524 . . . . _:b547271404 . . _:b547271810 . . _:b547271001 . _:b547271397 . _:b547271062 . . . _:b547271088 . . _:b547271127 . _:b547271656 . . _:b547271765 . _:b547271361 . . _:b547271585 . _:b547271110 . _:b547271384 . . . . _:b547271841 . _:b547271832 . _:b547271833 . _:b547271012 . _:b547271834 . . . _:b547271835 . _:b547271836 . _:b547271837 . _:b547271838 . _:b547271407 . . _:b547271839 . . _:b547271824 . _:b547271825 . _:b547271826 . _:b547271785 . _:b547271827 . _:b547271828 . _:b547271829 . _:b547271830 . _:b547271831 . _:b547271816 . _:b547271979 . _:b547271817 . _:b547271818 . _:b547271819 . _:b547271832 . _:b547271820 . _:b547271821 . _:b547271822 . _:b547271061 . _:b547271823 . . . _:b547271808 . _:b547271809 . _:b547271810 . _:b547271811 . _:b547271812 . _:b547271813 . . _:b547271814 . . _:b547271815 . _:b547271864 . _:b547271865 . _:b547271866 . . _:b547271867 . . _:b547271868 . _:b547271869 . _:b547271446 . _:b547271870 . _:b547271871 . _:b547271856 . _:b547271857 . _:b547271858 . _:b547271000 . _:b547271859 . _:b547271860 . . _:b547271564 . _:b547271861 . _:b547271862 . . _:b547271863 . _:b547271848 . _:b547271849 . _:b547271850 . _:b547271851 . . _:b547271852 . _:b547271853 . _:b547271854 . _:b547271855 . _:b547271840 . _:b547271537 . _:b547271841 . _:b547271842 . . _:b547271843 . _:b547271844 . _:b547271845 . _:b547271846 . _:b547271847 . . _:b547271896 . _:b547271897 . _:b547271898 . . _:b547271899 . _:b547271900 . _:b547271901 . _:b547271233 . _:b547271902 . _:b547271903 . _:b547271888 . _:b547271889 . _:b547271035 . _:b547271890 . _:b547271891 . _:b547271381 . _:b547271892 . _:b547271893 . _:b547271894 . _:b547271895 . _:b547271880 . _:b547271236 . _:b547271881 . _:b547271882 . _:b547271567 . _:b547271883 . _:b547271884 . _:b547271885 . _:b547271886 . _:b547271887 . _:b547271872 . _:b547271873 . _:b547271874 . _:b547271255 . _:b547271875 . _:b547271876 . _:b547271877 . _:b547271878 . _:b547271879 . . _:b547271928 . . _:b547271013 . _:b547271929 . . _:b547271930 . . _:b547271401 . _:b547271931 . . _:b547271932 . . . _:b547271933 . _:b547271934 . _:b547271935 . . _:b547271920 . . _:b547271921 . . _:b547271922 . . _:b547271923 . . _:b547271924 . _:b547271925 . _:b547271926 . _:b547271520 . _:b547271927 . _:b547271912 . _:b547271913 . _:b547271914 . _:b547271915 . _:b547271916 . _:b547271917 . _:b547271918 . _:b547271919 . _:b547271503 . _:b547271904 . _:b547271905 . . _:b547271906 . _:b547271907 . _:b547271265 . _:b547271908 . _:b547271909 . . _:b547271910 . . . . . _:b547271911 . . _:b547271704 . . _:b547271705 . _:b547271706 . . _:b547271707 . . . _:b547271708 . _:b547271059 . _:b547271709 . _:b547271710 . . _:b547271711 . _:b547271696 . _:b547271697 . _:b547271698 . _:b547271699 . _:b547271700 . _:b547271701 . _:b547271702 . _:b547271350 . _:b547271703 . _:b547271688 . _:b547271689 . _:b547271690 . _:b547271691 . _:b547271692 . _:b547271693 . . _:b547271694 . _:b547271695 . _:b547271680 . _:b547271681 . . _:b547271682 . _:b547271683 . _:b547271684 . _:b547271685 . _:b547271686 . _:b547271753 . _:b547271687 . _:b547271736 . _:b547271737 . _:b547271738 . _:b547271739 . _:b547271740 . _:b547271741 . . _:b547271742 . _:b547271743 . . _:b547271728 . _:b547271729 . _:b547271730 . . _:b547271731 . . _:b547271732 . _:b547271082 . _:b547271733 . _:b547271734 . _:b547271266 . _:b29539915 . _:b547271735 . _:b547271720 . _:b547271721 . _:b547271722 . _:b547271723 . _:b547271724 . . _:b547271725 . _:b547271156 . _:b547271726 . _:b547271727 . . _:b547271712 . _:b547271275 . _:b547271926 . _:b547271713 . _:b547271714 . _:b547271870 . _:b547271715 . _:b547271716 . . _:b547271717 . . . _:b547271718 . . _:b547271719 . _:b547271768 . _:b547271769 . _:b547271716 . _:b547271021 . _:b547271770 . _:b547271771 . _:b547271772 . _:b547271773 . _:b547271422 . . _:b547271774 . _:b547271775 . . _:b547271760 . _:b547271258 . _:b547271761 . . _:b547271762 . _:b547271763 . _:b547271764 . _:b547271281 . . _:b547271765 . _:b547271766 . _:b547271767 . _:b547271752 . _:b547271753 . _:b547271754 . _:b547271755 . _:b547271756 . _:b547271757 . . _:b547271758 . _:b547271759 . _:b547271601 . _:b547271744 . _:b547271579 . . _:b547271745 . _:b547271746 . . _:b547271747 . _:b547271748 . _:b547271749 . _:b547271750 . _:b547271751 . _:b547271929 . _:b547271800 . . _:b547271801 . _:b547271261 . _:b547271802 . _:b547271762 . _:b547271345 . _:b547271803 . _:b547271804 . _:b547271329 . . _:b547271805 . _:b547271806 . _:b547271936 . _:b547271807 . _:b547271792 . . _:b547271793 . _:b547271794 . _:b547271795 . _:b547271796 . _:b547271822 . _:b547271797 . . _:b547271798 . _:b547271799 . _:b547271784 . _:b547271785 . _:b547271786 . _:b547271787 . _:b547271788 . _:b547271789 . . _:b547271790 . _:b547271791 . _:b547271776 . _:b547271777 . _:b547271778 . _:b547271779 . _:b29539910 "outlook" . _:b547271780 . . _:b547271781 . _:b547271640 . _:b547271782 . _:b547271783 . _:b547271782 . . . _:b547271911 . . . . _:b547271336 . . _:b547271910 . . _:b547271339 . _:b547271222 . . . . _:b547271481 . . _:b547271669 . . . _:b547271036 . . . . . . _:b547271431 . . . . _:b547270997 . . _:b547271632 . . _:b547271735 . _:b547271102 . _:b547271374 . . . . . _:b547271944 . . . _:b547271898 . _:b547271688 . _:b547271298 . . . _:b29539907 "The preparation of PEGdiPDA, photodegradable hydrogels was adapted from previously described protocols. >>20<<,25 Briefly, PEGdiPDA was co-polymerized with poly(ethylene glycol) monoacrylate (PEGA; Mn ~ 400 Da; Monomer-Polymer and Dajac Laboratories, Inc." . . _:b547271087 . . _:b547271589 . _:b29539923 "mechanical dosing on dynamic substrates" . _:b547271960 . _:b547271961 . _:b547271962 . _:b547271963 . _:b547271964 . _:b547271965 . _:b547271966 . _:b547271967 . _:b547271952 . _:b547271826 . _:b547271953 . . _:b547271954 . _:b547271955 . _:b547271956 . _:b547271957 . _:b547271958 . _:b547271959 . _:b547271944 . _:b547271945 . . _:b547271946 . _:b547271268 . _:b547271947 . _:b547271948 . _:b547271949 . . _:b547271950 . _:b547271951 . _:b547271936 . _:b547271937 . _:b547271938 . _:b547271939 . _:b547271347 . _:b547271940 . _:b547271972 . _:b547271941 . _:b547271764 . _:b547271942 . _:b547271440 . _:b547271943 . _:b547271776 . . . _:b547271612 . . _:b547271514 . _:b547271416 . . _:b547271521 . . _:b547271043 . _:b547271976 . _:b547271435 . _:b547271977 . _:b547271551 . _:b547271978 . _:b547271979 . _:b547271120 . _:b547271980 . _:b547271602 . _:b547271064 . _:b547271955 . _:b547271981 . _:b547271968 . _:b547271804 . _:b547271969 . _:b547271970 . _:b547271971 . _:b547271629 . _:b547271972 . _:b547271973 . _:b547271974 . _:b547271975 . . . _:b547271025 . . _:b547271493 . . _:b547271151 . _:b29539922 "the nuclei for DT7 / So3. Additional staining confirmed that TAZ localized with YAP (cytoplasmic on soft substrates and nuclear on stiff substrates); YAP/TAZ together form a functional mechanosensitive entity (Supplementary Fig. S7).15,>>16<< These findings demonstrate that extended culture on supraphysiologically stiff substrata (TCPS) leads to persistent activation of YAP in hMSCs even after transfer to de-activating, soft hydrogels." . . . . _:b547271757 . . _:b547271028 . _:b547271542 . . _:b547271527 . . _:b547271263 . _:b547271418 . . _:b547271792 . . _:b547271662 . _:b547271942 . . . . _:b547271696 . _:b547271646 . _:b29539924 . . . . . . _:b547271962 . _:b547271811 . . _:b547271925 . . _:b547271517 . . . _:b547271166 . . _:b547271951 . _:b547271565 . _:b547271649 . _:b547271346 . _:b547271651 . . . _:b547271210 . _:b547271882 . _:b547271835 . . _:b547271733 . _:b547271167 . _:b547271744 . _:b547271432 . _:b547271581 . _:b547271665 . . _:b547271022 . _:b547271511 . . . _:b547271570 . . _:b547271818 . . _:b547271342 . _:b547271072 . . . _:b547271685 "2"^^ . _:b547271866 . _:b29539907 . . _:b547271684 "2"^^ . . . . _:b547271687 "2"^^ . _:b547271686 "2"^^ . _:b547271681 "2"^^ . _:b547271126 . _:b547271680 "2"^^ . . . _:b547271683 "2"^^ . _:b547271042 . _:b547271168 . . . . _:b547271682 "2"^^ . . _:b547271693 "2"^^ . _:b547271285 . _:b547271692 "2"^^ . . _:b547271695 "2"^^ . . _:b547271694 "2"^^ . _:b547271689 "2"^^ . _:b547271731 . _:b547271977 . _:b547271688 "2"^^ . _:b547271685 . _:b547271814 . _:b547271691 "2"^^ . _:b547271684 . . . _:b547271690 "2"^^ . . _:b547271687 . . _:b547271701 "2"^^ . _:b547271686 . . _:b547271768 . . _:b547271700 "2"^^ . _:b547271360 . _:b547271681 . _:b547271703 "2"^^ . _:b547271868 . _:b547271680 . _:b547271679 . _:b547271892 . _:b547271702 "2"^^ . _:b547271683 . _:b547271697 "2"^^ . _:b547271682 . . _:b547271696 "2"^^ . _:b547271693 . _:b547271699 "2"^^ . _:b547271692 . . . _:b547271698 "2"^^ . _:b547271695 . _:b547271709 "2"^^ . _:b547271694 . . _:b547271060 . _:b547271708 "2"^^ . _:b547271689 . _:b547271122 . _:b547271711 "2"^^ . _:b547271688 . . _:b547271710 "2"^^ . _:b547271691 . _:b547271051 . _:b547271705 "2"^^ . _:b547271197 . _:b547271690 . _:b547271574 . . _:b547271704 "2"^^ . . _:b547271701 . _:b547271562 . _:b547271242 . _:b547271706 . _:b547271707 "2"^^ . _:b547271700 . _:b547271292 . _:b547271164 . _:b547271706 "2"^^ . _:b547271703 . _:b29539926 "culture (Supplementary Fig. S4). The unique use of light enables precise control over the hydrogel modulus, as the photoreaction ceases when the light is turned off and the extent of reaction is governed by the dose of light (Fig. 2b).>>18<<,21 Exploiting this property, initially stiff hydrogels were formed into a set of discrete hydrogels with defined moduli (E ~2, 4, 6, and 10 kPa; Fig." . _:b547271959 . _:b547271338 . _:b547271717 "2"^^ . _:b547271702 . _:b547271716 "2"^^ . _:b547271697 . . _:b547271308 . _:b547271479 . _:b547271719 "2"^^ . _:b547271696 . _:b547271718 "2"^^ . _:b547271699 . _:b547271713 "2"^^ . _:b547271698 . _:b547271353 . _:b547271712 "2"^^ . _:b547271860 . _:b547271709 . . _:b547271092 . _:b547271100 . _:b547271715 "2"^^ . _:b547271494 . _:b547271155 . _:b547271708 . _:b547271106 . _:b547271714 "2"^^ . _:b547271711 . . . . _:b547271725 "2"^^ . _:b547271710 . _:b547271724 "2"^^ . _:b547271705 . _:b547271215 . _:b547271854 . . _:b547271727 "2"^^ . _:b547271704 . _:b547271666 . _:b547271726 "2"^^ . . _:b547271788 . _:b547271707 . . _:b547271721 "2"^^ . _:b547271706 . _:b547271815 . _:b547271720 "2"^^ . _:b547271450 . _:b547271717 . _:b547271385 . _:b547271723 "2"^^ . . _:b547271716 . _:b547271722 "2"^^ . _:b547271719 . . _:b547271733 "2"^^ . _:b547271718 . . . . _:b547271480 . _:b547271732 "2"^^ . _:b547271713 . _:b547271133 . _:b547271735 "2"^^ . _:b547271712 . . _:b547271301 . _:b547271734 "2"^^ . _:b547271715 . _:b29539919 "in hMSCs on substrata with stiff moduli (E ~ 40 kPa) and de-activated (located in the cytoplasm) on substrata with soft moduli (E ~ 1 kPa); further, this work showed that YAP/TAZ activation correlated with osteogenic differentiation.15,>>16<< Thus, we investigated the role of YAP location, or activation, during mechanical dosing and in the mechanical memory of hMSCs." . _:b547271729 "2"^^ . _:b547271714 . . . _:b547271728 "2"^^ . _:b547271725 . _:b547271731 "2"^^ . _:b547271508 . _:b547271724 . . _:b547271730 "2"^^ . _:b547271727 . _:b547271457 . _:b547271741 "2"^^ . _:b547271751 . . _:b547271726 . _:b547271369 . . _:b547271740 "2"^^ . _:b547271531 . _:b547271704 . _:b547271721 . . . . _:b547271743 "2"^^ . _:b547271720 . _:b547271596 . _:b547271714 . _:b547271742 "2"^^ . _:b547271723 . _:b547271004 . _:b547271737 "2"^^ . _:b547271722 . _:b547271736 "2"^^ . _:b547271664 . _:b547271733 . . _:b547271739 "2"^^ . _:b547271732 . _:b547271738 "2"^^ . . _:b547271735 . . . _:b547271749 "2"^^ . . _:b547271734 . _:b547271748 "2"^^ . _:b547271729 . _:b547271751 "2"^^ . _:b547271728 . _:b547271750 "2"^^ . _:b547271731 . _:b547271529 . _:b547271745 "2"^^ . . _:b547271730 . _:b547271549 . _:b547271744 "2"^^ . _:b547271741 . _:b547271747 "2"^^ . _:b547271740 . _:b547271240 . _:b547271746 "2"^^ . _:b547271743 . _:b547271566 . _:b547271717 . _:b547271757 "2"^^ . _:b547271742 . _:b547271756 "2"^^ . _:b547271737 . _:b547271219 . _:b547271325 . . . _:b547271436 . _:b547271759 "2"^^ . . _:b547271736 . _:b29539914 "changes induced by constitutive YAP activation mediated genetically, epigenetically, or structurally?30 There is strong evidence that YAP/TAZ mechanotransduction depends directly on the organization of the actin cytoskeleton.15,>>16<< In preliminary experiments in this setting, the effects of mechanical dosing persisted even after disruption of the actin cytoskeleton with latrunculin A (Supplementary Fig." . _:b547271626 . _:b547271758 "2"^^ . _:b547271739 . _:b547271753 "2"^^ . _:b547271738 . _:b547271752 "2"^^ . _:b547271749 . _:b547271014 . _:b547271755 "2"^^ . _:b547271748 . _:b547271754 "2"^^ . _:b547271751 . _:b547271725 . _:b547271765 "2"^^ . _:b547271750 . _:b29539911 "this manuscript suggests a temporal role in cellular mechanotransduction that involves the history of a cell\u2019s microenvironment. YAP shuttles between the cytoplasm and nucleus, as a mediator of extracellular microenvironmental signals.>>16<< We show that continual exposure to culture environments that activate YAP transiently can lead to constitutive activation." . _:b547271764 "2"^^ . _:b547271745 . _:b547271238 . _:b547271767 "2"^^ . _:b547271744 . _:b547271178 . _:b547271766 "2"^^ . . _:b547271747 . . _:b547271761 "2"^^ . _:b547271746 . . _:b547271760 "2"^^ . . _:b547271757 . _:b547271304 . . _:b547271763 "2"^^ . _:b547271756 . . _:b547271920 . . _:b547271762 "2"^^ . _:b547271759 . _:b547271773 "2"^^ . _:b547271758 . _:b547271245 . . . _:b547271907 . _:b547271772 "2"^^ . _:b547271654 . _:b547271753 . _:b547271530 . _:b547271775 "2"^^ . _:b547271752 . . _:b547271774 "2"^^ . . _:b547271755 . _:b547271769 "2"^^ . . _:b547271754 . . _:b547271768 "2"^^ . . _:b547271765 . _:b547271771 "2"^^ . _:b547271764 . . . . _:b547271770 "2"^^ . _:b547271096 . _:b547271767 . _:b547271633 . _:b547271781 "2"^^ . _:b547271766 . _:b547271780 "2"^^ . _:b547271761 . . _:b547271783 "2"^^ . _:b547271760 . . _:b547271782 "2"^^ . . _:b547271763 . _:b547271777 "2"^^ . _:b547271762 . _:b547271890 . . _:b547271776 "2"^^ . _:b547271773 . _:b547271779 "2"^^ . _:b547271267 . _:b547271772 . . _:b547271778 "2"^^ . _:b547271775 . _:b547271789 "2"^^ . _:b547271774 . . _:b547271234 . _:b547271788 "2"^^ . _:b547271769 . . . _:b547271791 "2"^^ . . _:b547271768 . . . _:b547271790 "2"^^ . . _:b547271771 . _:b547271419 . _:b547271785 "2"^^ . _:b547271770 . . _:b547271367 . _:b547271784 "2"^^ . _:b547271781 . . _:b547271787 "2"^^ . _:b547271780 . _:b29539906 "peptide OOGRGDSG (diethylene glycol-diethylene glycol-glycine-arginine-glycine-aspartic acid-serine-glycine) was synthesized (Protein Technologies Tribute peptide synthesizer) through Fmoc solid-phase methodology and HATU activation.>>26<< Acrylic acid was coupled on resin to the N-terminal amine with HATU to synthesize Acryl-OOGRGDSG." . _:b547271786 "2"^^ . _:b547271783 . _:b547271547 . _:b547271797 "2"^^ . _:b547271251 . _:b547271782 . . _:b547271607 . _:b547271269 . _:b547271796 "2"^^ . _:b547271777 . _:b547270991 "22"^^ . . _:b547271799 "2"^^ . . _:b547271776 . . _:b547270990 "28"^^ . _:b547270999 . _:b547271798 "2"^^ . _:b547271395 . _:b547271779 . . _:b547271274 . _:b547271793 "2"^^ . . _:b547271778 . _:b547271193 . _:b547270993 "19"^^ . _:b547271792 "2"^^ . . _:b547271789 . _:b547271020 . _:b547271795 "2"^^ . _:b547270992 "20"^^ . _:b547271788 . _:b547271148 . _:b547271557 . . _:b547271794 "2"^^ . . _:b547271791 . . _:b547270994 "18"^^ . . . _:b547271805 "2"^^ . _:b547271790 . _:b547270995 "17"^^ . _:b547271260 . _:b547271804 "2"^^ . _:b547271924 . _:b547271315 . _:b547271785 . _:b547270996 "17"^^ . _:b29539925 . _:b547270989 "46"^^ . _:b547270997 "14"^^ . _:b547271526 . _:b547271807 "2"^^ . _:b547271784 . _:b547270998 "14"^^ . _:b547271712 . _:b547271806 "2"^^ . . _:b547271787 . _:b547271454 . _:b547270999 "14"^^ . _:b547271801 "2"^^ . _:b547271786 . . _:b547271000 "14"^^ . _:b547270988 "63"^^ . _:b547271800 "2"^^ . _:b547271007 "12"^^ . _:b547271797 . _:b547271803 "2"^^ . _:b547271006 "12"^^ . _:b547271796 . _:b547271802 "2"^^ . _:b547271005 "12"^^ . _:b547271799 . . . _:b547271813 "2"^^ . . _:b547271798 . _:b547271004 "12"^^ . _:b547271831 . . _:b547271812 "2"^^ . _:b547271793 . _:b547271003 "13"^^ . _:b547271780 . _:b547271815 "2"^^ . _:b547271792 . _:b547271187 . _:b547271628 . _:b547271002 "13"^^ . _:b547271814 "2"^^ . _:b547271752 . _:b547271795 . _:b547271001 "13"^^ . _:b547271809 "2"^^ . _:b547271794 . . _:b547271015 "10"^^ . _:b547271808 "2"^^ . _:b547271805 . _:b547271056 . _:b547271014 "10"^^ . _:b547271098 . _:b547271811 "2"^^ . . _:b547271804 . . . . _:b547271013 "10"^^ . _:b547271810 "2"^^ . _:b547271807 . . _:b547271012 "10"^^ . _:b547271821 "2"^^ . _:b547271830 . _:b547271806 . _:b547271011 "10"^^ . _:b547271820 "2"^^ . _:b547271801 . _:b547271010 "10"^^ . _:b547271630 . _:b547271823 "2"^^ . _:b547271358 . _:b547271800 . . _:b547271009 "11"^^ . _:b547271822 "2"^^ . _:b547271803 . . _:b547271296 . _:b547271817 "2"^^ . _:b547271008 "12"^^ . _:b547271802 . _:b547271816 "2"^^ . _:b547271813 . _:b547271022 "9"^^ . . . _:b547271819 "2"^^ . _:b547271812 . _:b547271023 "9"^^ . _:b547271694 . _:b547271818 "2"^^ . . _:b547271815 . _:b547271761 . _:b547271020 "9"^^ . . . _:b547271829 "2"^^ . . _:b547271814 . _:b547271021 "9"^^ . . _:b547271828 "2"^^ . _:b547271809 . . _:b547271018 "9"^^ . . . _:b547271807 . _:b547271831 "2"^^ . _:b547271808 . _:b547271019 "9"^^ . _:b547271380 . . _:b547271476 . . _:b547271830 "2"^^ . _:b547271811 . _:b547271016 "9"^^ . . _:b547271825 "2"^^ . _:b547271810 . _:b547271017 "9"^^ . _:b547271824 "2"^^ . _:b547271030 "8"^^ . _:b547271821 . _:b547271317 . . _:b547271827 "2"^^ . _:b547271031 "8"^^ . _:b547271820 . _:b547271826 "2"^^ . _:b547271028 "8"^^ . _:b547271899 . _:b547271823 . _:b547271411 . _:b547271837 "2"^^ . _:b547271029 "8"^^ . _:b547271822 . _:b547271836 "2"^^ . _:b547271026 "8"^^ . _:b547271817 . _:b547271291 . _:b547271839 "2"^^ . _:b547271027 "8"^^ . _:b547271816 . . _:b547271546 . _:b547271838 "2"^^ . . _:b547271819 . _:b547271024 "9"^^ . _:b547271024 . _:b547271833 "2"^^ . _:b547271252 . _:b547271818 . _:b547271798 . _:b547271025 "8"^^ . _:b547271038 "7"^^ . _:b547271832 "2"^^ . _:b547271829 . . _:b547271279 . _:b547271835 "2"^^ . _:b547271074 . _:b547271039 "7"^^ . _:b547271828 . _:b547271497 . _:b547271834 "2"^^ . _:b547271036 "8"^^ . _:b547271831 . . _:b547271845 "2"^^ . _:b547271037 "7"^^ . _:b547271830 . . _:b547271387 . _:b547271844 "2"^^ . _:b547271034 "8"^^ . _:b547271825 . . . _:b547271932 . _:b547271847 "2"^^ . _:b547271035 "8"^^ . _:b547271909 . _:b547271824 . . . _:b547271846 "2"^^ . _:b547271032 "8"^^ . _:b547271827 . _:b547271561 . _:b547271841 "2"^^ . _:b547271033 "8"^^ . _:b547271826 . _:b547271492 . . _:b547271046 "7"^^ . _:b547271922 . _:b547271840 "2"^^ . _:b547271837 . . _:b547271510 . _:b547271047 "7"^^ . _:b547271846 . _:b547271843 "2"^^ . _:b547271836 . . _:b547271044 "7"^^ . _:b547271842 "2"^^ . _:b547271839 . _:b547271045 "7"^^ . _:b547271853 "2"^^ . _:b547271838 . _:b547271552 . _:b547271042 "7"^^ . _:b547271852 "2"^^ . . _:b547271833 . _:b547271249 . _:b547271031 . _:b547271855 "2"^^ . . _:b547271043 "7"^^ . _:b547271832 . . _:b547271040 "7"^^ . _:b547271854 "2"^^ . _:b547271835 . _:b547271658 . _:b547271041 "7"^^ . _:b547271849 "2"^^ . _:b547271834 . _:b547271054 "6"^^ . _:b547271848 "2"^^ . . _:b547271845 . . _:b547271055 "6"^^ . . _:b547271851 "2"^^ . _:b547271844 . _:b547271064 . _:b547271052 "6"^^ . _:b547271065 . _:b547271850 "2"^^ . . _:b547271066 . _:b547271847 . _:b547271067 . _:b547271068 . _:b547271053 "6"^^ . . _:b547271861 "2"^^ . _:b547271069 . _:b547271070 . _:b547271846 . _:b547271002 . _:b547271071 . _:b547271056 . _:b547271050 "6"^^ . _:b547271057 . _:b547271860 "2"^^ . _:b547271058 . _:b547271841 . . _:b547271059 . _:b547271060 . _:b547271051 "6"^^ . _:b547271061 . _:b547271863 "2"^^ . _:b547271062 . _:b547271840 . _:b547271063 . . _:b547271048 . _:b547271049 . _:b547271862 "2"^^ . _:b547271048 "7"^^ . _:b547271050 . _:b547271843 . _:b547271051 . _:b547271052 . _:b547271053 . _:b547271857 "2"^^ . _:b547271049 "7"^^ . _:b547271054 . _:b547271842 . _:b547271055 . _:b547271040 . _:b547271062 "6"^^ . _:b547271041 . _:b547271856 "2"^^ . _:b547271027 . _:b547271853 . _:b547271042 . _:b547271043 . _:b547271044 . . _:b547271063 "6"^^ . _:b547271045 . _:b547271859 "2"^^ . _:b547271046 . _:b547271861 . _:b547271852 . _:b547271047 . _:b547271096 . _:b547271060 "6"^^ . . _:b547271858 "2"^^ . _:b547271097 . _:b547271098 . _:b547271855 . _:b547271099 . _:b547271100 . _:b547271232 . _:b547271061 "6"^^ . _:b547271101 . _:b547271869 "2"^^ . _:b547271102 . _:b547271854 . . _:b547271103 . _:b547271088 . _:b547271058 "6"^^ . _:b547271089 . _:b547271868 "2"^^ . _:b547271090 . _:b547271849 . _:b547271091 . _:b547271010 . _:b547271917 . _:b547271059 "6"^^ . _:b547271092 . _:b547271093 . _:b547271871 "2"^^ . _:b547271094 . _:b547271848 . _:b547271095 . _:b547271080 . _:b547271373 . _:b547271056 "6"^^ . . _:b547271870 "2"^^ . _:b547271081 . _:b547271082 . _:b547271851 . _:b547271149 . . _:b547271083 . _:b547271084 . _:b547271057 "6"^^ . _:b547271085 . _:b547271865 "2"^^ . . _:b547271850 . _:b547271086 . _:b547271087 . _:b547271072 . _:b547271528 . _:b547271070 "6"^^ . _:b547271073 . _:b547271864 "2"^^ . _:b547271074 . _:b547271861 . _:b547271075 . . _:b547271076 . _:b547271077 . _:b547271867 "2"^^ . . _:b547271860 . _:b547271078 . _:b547271079 . _:b547271316 . _:b547271128 . _:b547271068 "6"^^ . . _:b547271866 "2"^^ . _:b547271129 . _:b547271130 . _:b547271863 . _:b547271131 . _:b547271071 "5"^^ . _:b547271132 . _:b547271069 "6"^^ . _:b547271133 . _:b547271877 "2"^^ . _:b547271134 . _:b547271862 . _:b547271829 . _:b547271396 . _:b547271047 . _:b547271135 . . . _:b547271066 "6"^^ . _:b547271120 . _:b547271121 . _:b547271876 "2"^^ . . _:b547271122 . _:b547271857 . _:b547271123 . _:b547271124 . _:b29539925 . _:b547271067 "6"^^ . _:b547271125 . _:b547271938 . _:b547271879 "2"^^ . _:b547271126 . _:b547271856 . _:b547271613 . . _:b547271127 . _:b547271220 . _:b547271064 "6"^^ . _:b547271112 . _:b547271113 . _:b547271878 "2"^^ . _:b547271376 . _:b547271114 . _:b547271859 . _:b547271115 . _:b547271116 . _:b547271065 "6"^^ . _:b547271117 . _:b547271873 "2"^^ . _:b547271118 . _:b547271858 . _:b547271119 . _:b547271104 . _:b547271105 . _:b547271872 "2"^^ . _:b547271106 . _:b547271869 . _:b547271107 . _:b547271184 . _:b547271077 "5"^^ . _:b547271108 . _:b547271109 . _:b547271875 "2"^^ . . _:b547271110 . _:b547271868 . _:b547271111 . _:b547271076 "5"^^ . _:b547271160 . _:b547271161 . _:b547271874 "2"^^ . _:b547271162 . _:b547271871 . _:b547271163 . _:b547271079 "5"^^ . _:b547271164 . _:b547271165 . _:b547271885 "2"^^ . _:b547271166 . _:b547271870 . _:b547271167 . _:b547271078 "5"^^ . _:b547271152 . _:b547271153 . _:b547271884 "2"^^ . _:b547271154 . _:b547271865 . _:b547271104 . _:b547271155 . _:b547271073 "5"^^ . _:b547271156 . _:b547271157 . _:b547271887 "2"^^ . . _:b547271864 . _:b547271158 . _:b547271159 . _:b547271072 "5"^^ . _:b547271144 . _:b29539906 . _:b547271145 . _:b547271886 "2"^^ . _:b547271639 . _:b547271146 . _:b547271867 . _:b547271147 . _:b547271075 "5"^^ . _:b547271148 . _:b547271149 . _:b547271881 "2"^^ . . _:b547271150 . _:b547271866 . _:b547271151 . _:b547271074 "5"^^ . _:b547271136 . _:b547271137 . _:b547271880 "2"^^ . _:b547271138 . _:b547271877 . . _:b547271139 . _:b547271085 "5"^^ . _:b547271140 . . _:b547271883 "2"^^ . _:b547271141 . _:b547271142 . _:b547271876 . _:b547271143 . _:b547271084 "5"^^ . . _:b547271882 "2"^^ . _:b547271879 . _:b547271087 "5"^^ . _:b547271893 "2"^^ . _:b547271878 . _:b547271086 "5"^^ . _:b547271421 . _:b547271650 . _:b547271892 "2"^^ . _:b547271466 . _:b547271873 . _:b547271081 "5"^^ . . _:b547271895 "2"^^ . . _:b29539918 "in hMSCs on substrata with stiff moduli (E ~ 40 kPa) and de-activated (located in the cytoplasm) on substrata with soft moduli (E ~ 1 kPa); further, this work showed that YAP/TAZ activation correlated with osteogenic differentiation.>>15<<,16 Thus, we investigated the role of YAP location, or activation, during mechanical dosing and in the mechanical memory of hMSCs." . _:b547271872 . _:b547271080 "5"^^ . _:b547271894 "2"^^ . _:b547271875 . _:b547271083 "5"^^ . . _:b547271889 "2"^^ . _:b547271874 . _:b547271082 "5"^^ . . _:b547271247 . _:b547271888 "2"^^ . _:b547271885 . _:b547271093 "5"^^ . _:b547271434 . _:b547271891 "2"^^ . _:b547271872 . _:b547271884 . _:b547271092 "5"^^ . _:b547271241 . _:b547271890 "2"^^ . . _:b547271887 . _:b547271095 "5"^^ . _:b547271678 . _:b547271901 "2"^^ . . _:b547271886 . _:b547271094 "5"^^ . _:b547271745 . _:b547271900 "2"^^ . _:b547271505 . _:b547271881 . . _:b547271089 "5"^^ . _:b547271231 . _:b547271672 . _:b547271903 "2"^^ . . _:b547271880 . _:b547271088 "5"^^ . . . _:b547271902 "2"^^ . _:b547271883 . _:b547271091 "5"^^ . _:b547271259 . _:b547271897 "2"^^ . _:b547271882 . _:b547271090 "5"^^ . . _:b547271896 "2"^^ . _:b547271737 . _:b547271101 "4"^^ . _:b547271893 . _:b547271844 . _:b547271899 "2"^^ . _:b547271100 "4"^^ . _:b547271892 . _:b547271451 . _:b547271000 . . _:b547271001 . _:b547271898 "2"^^ . _:b547271002 . _:b547271895 . _:b547271103 "4"^^ . . _:b547271003 . _:b547271004 . _:b547271005 . _:b547271909 "2"^^ . _:b547271006 . _:b547271894 . _:b547271102 "4"^^ . _:b547271007 . _:b547271495 . _:b547270992 . _:b547271509 . _:b547270993 . _:b547271908 "2"^^ . _:b547270994 . _:b547271889 . _:b547270995 . _:b547271097 "5"^^ . . _:b547270996 . _:b547270997 . _:b547271911 "2"^^ . . _:b547271888 . _:b547271723 . _:b547270998 . _:b547270999 . _:b547271096 "5"^^ . . _:b547271910 "2"^^ . _:b547271099 "4"^^ . _:b547271891 . _:b547270988 . _:b547270989 . _:b547271905 "2"^^ . _:b547270990 . _:b547271890 . _:b547271098 "4"^^ . _:b547270991 . _:b547271904 "2"^^ . _:b547271109 "4"^^ . _:b547271901 . _:b547271560 . . _:b547271907 "2"^^ . _:b547271108 "4"^^ . _:b547271900 . . _:b547271032 . _:b547271033 . _:b547271906 "2"^^ . _:b547271034 . _:b547271903 . . _:b547271111 "4"^^ . _:b547271035 . _:b547271036 . _:b547271037 . _:b547271917 "2"^^ . _:b547271038 . _:b547271902 . _:b547271110 "4"^^ . _:b547271039 . _:b547271024 . _:b547271025 . _:b547271916 "2"^^ . _:b547271026 . _:b547271897 . _:b547271105 "4"^^ . _:b547271027 . _:b547271028 . _:b547271029 . _:b547271919 "2"^^ . _:b547271030 . _:b547271896 . _:b547271104 "4"^^ . _:b547271031 . _:b547271016 . _:b547271017 . _:b547271918 "2"^^ . _:b547271018 . _:b547271899 . _:b547271107 "4"^^ . . _:b547271398 . _:b547271019 . _:b547271020 . _:b547271021 . _:b547271913 "2"^^ . _:b547271022 . _:b547271898 . _:b547271106 "4"^^ . _:b547271023 . _:b547271008 . _:b547271053 . _:b547271912 "2"^^ . . . _:b547271009 . _:b547271010 . _:b547271909 . _:b547271117 "4"^^ . _:b547271011 . _:b547271012 . _:b547271013 . _:b547271915 "2"^^ . _:b547271014 . _:b547271908 . _:b547271116 "4"^^ . _:b547271015 . _:b547271320 . _:b29539916 . _:b547271321 . _:b547271914 "2"^^ . _:b547271322 . _:b547271911 . _:b547271119 "4"^^ . _:b547271323 . _:b547271851 . . _:b547271324 . _:b547271325 . _:b547271925 "2"^^ . _:b547271326 . _:b547271910 . _:b547271118 "4"^^ . _:b547271327 . _:b547271312 . _:b547271008 . _:b547271924 "2"^^ . _:b547271781 . _:b547271313 . _:b547271314 . _:b547271905 . _:b547271113 "4"^^ . . _:b29539910 . _:b547271315 . _:b547271695 . . _:b547271316 . _:b29539925 . _:b547271317 . _:b547271927 "2"^^ . _:b547271318 . _:b547271904 . _:b547271112 "4"^^ . _:b547271319 . _:b547271304 . _:b547271609 . _:b547271305 . _:b547271926 "2"^^ . _:b547271306 . _:b547271907 . _:b547271543 . _:b547271115 "4"^^ . _:b547271225 . _:b547271575 . _:b547271307 . _:b547271308 . _:b29539920 . _:b547271196 . _:b547271921 "2"^^ . _:b547271131 . _:b547271309 . . _:b547271310 . _:b547271906 . _:b547271114 "4"^^ . _:b547271311 . _:b29539923 . _:b547271296 . _:b547271297 . _:b547271920 "2"^^ . _:b547271298 . _:b547271917 . _:b547271125 "4"^^ . _:b547271299 . _:b29539922 . _:b547271163 . _:b29539927 . _:b547271300 . _:b547271417 . _:b547271301 . _:b547271923 "2"^^ . . _:b547271302 . _:b547271916 . _:b547271124 "4"^^ . _:b547271303 . _:b547271352 . . _:b547271353 . _:b547271922 "2"^^ . _:b547271743 . _:b547271354 . _:b547271919 . _:b547271127 "4"^^ . _:b547271355 . _:b547271970 . _:b547271356 . _:b547271357 . _:b547271933 "2"^^ . . _:b547271918 . _:b547271126 "4"^^ . _:b547271358 . _:b547271359 . _:b547271344 . . _:b547271345 . _:b547271932 "2"^^ . _:b547271346 . _:b547271913 . _:b547271121 "4"^^ . _:b547271347 . _:b547271348 . _:b547271312 . _:b547271349 . _:b547271935 "2"^^ . _:b547271350 . _:b547271912 . _:b547271120 "4"^^ . _:b547271351 . _:b547271336 . _:b547271337 . _:b547271934 "2"^^ . _:b547271338 . _:b547271915 . _:b547271123 "4"^^ . _:b547271339 . _:b547271340 . _:b547271341 . _:b547271929 "2"^^ . _:b547271342 . _:b547271914 . _:b547271122 "4"^^ . _:b547271343 . _:b547271328 . . _:b547271928 "2"^^ . _:b547271329 . _:b547271330 . _:b547271925 . _:b547271133 "4"^^ . _:b547271331 . _:b547271332 . _:b547271715 . _:b547271333 . _:b547271931 "2"^^ . _:b547271334 . _:b547271924 . _:b547271700 . _:b547271132 "4"^^ . . _:b547271335 . _:b547271384 . _:b547271385 . _:b547271930 "2"^^ . _:b547271500 . _:b547271386 . _:b547271927 . _:b547271135 "4"^^ . _:b547271387 . _:b547271388 . _:b547271389 . _:b547271941 "2"^^ . . _:b547271390 . _:b547271926 . _:b547271134 "4"^^ . _:b547271391 . _:b547271376 . _:b547271377 . _:b547271940 "2"^^ . _:b547271378 . _:b547271921 . _:b547271129 "4"^^ . _:b547271379 . . _:b547271380 . _:b547271381 . _:b547271943 "2"^^ . _:b547271382 . _:b547271920 . _:b547271128 "4"^^ . _:b547271286 . _:b547271383 . _:b547271368 . _:b547271369 . _:b547271942 "2"^^ . _:b547271370 . _:b547271923 . _:b547271131 "4"^^ . _:b547271371 . _:b547271871 . _:b547271372 . . _:b547271937 "2"^^ . _:b547271373 . _:b547271374 . _:b547271922 . _:b547271130 "4"^^ . _:b547271375 . . _:b547271360 . _:b547271361 . _:b547271936 "2"^^ . _:b547271362 . _:b547271933 . _:b547271141 "4"^^ . _:b547271363 . _:b547271364 . _:b547271365 . _:b547271939 "2"^^ . _:b547271366 . _:b547271932 . _:b547271140 "4"^^ . . _:b547271367 . _:b547271416 . _:b547271417 . _:b547271938 "2"^^ . _:b547271418 . _:b547271935 . _:b547271143 "4"^^ . _:b547271419 . _:b547271420 . _:b547271421 . _:b547271949 "2"^^ . _:b547271422 . _:b547271934 . _:b547271142 "4"^^ . _:b547271423 . _:b547271408 . _:b547271428 . _:b547271409 . _:b547271948 "2"^^ . _:b547271410 . _:b547271929 . _:b547271701 . _:b547271137 "4"^^ . _:b547271411 . . _:b547271412 . . _:b547271951 "2"^^ . _:b547271413 . . _:b547271928 . _:b547271136 "4"^^ . _:b547271414 . _:b547271415 . _:b29539911 . _:b547271400 . _:b547271401 . _:b547271950 "2"^^ . _:b547271402 . _:b547271931 . _:b547271769 . _:b547271139 "4"^^ . _:b547271403 . _:b547270993 . _:b29539903 . _:b547271404 . _:b547271405 . _:b547271945 "2"^^ . _:b547271406 . _:b547271930 . _:b547271138 "4"^^ . _:b547271407 . _:b547271392 . _:b547271393 . _:b547271944 "2"^^ . _:b547271394 . _:b547271429 . . _:b547271149 "4"^^ . _:b547271395 . _:b29539914 . _:b547271396 . _:b547271397 . _:b547271947 "2"^^ . . _:b547271428 . _:b547271148 "4"^^ . _:b547271398 . . _:b547271399 . _:b547271192 . _:b547271193 . _:b547271946 "2"^^ . _:b547271194 . _:b547271431 . _:b547271151 "4"^^ . _:b547271195 . _:b547271196 . _:b547271197 . _:b547271957 "2"^^ . _:b547271198 . _:b547271430 . _:b547271150 "4"^^ . _:b547271218 . _:b547271199 . _:b547271184 . . _:b547271956 "2"^^ . _:b547271185 . _:b547271186 . _:b547271425 . _:b547271145 "4"^^ . _:b547271187 . _:b547271188 . _:b547271189 . _:b547271959 "2"^^ . _:b547271738 . _:b547271190 . _:b547271424 . _:b547271144 "4"^^ . _:b547271191 . _:b29539919 . . _:b547271176 . . _:b547271958 "2"^^ . . _:b547271177 . _:b547271178 . _:b547271427 . _:b547271147 "4"^^ . _:b547271058 . _:b547271099 . _:b547271179 . _:b547271180 . . _:b547271181 . _:b547271953 "2"^^ . _:b547271182 . _:b547271426 . _:b547271146 "4"^^ . _:b547271183 . _:b547271685 . _:b547271168 . _:b547271209 . _:b547271952 "2"^^ . _:b547271169 . _:b547271170 . _:b547271437 . _:b547271157 "4"^^ . _:b547271171 . _:b547271172 . _:b547271067 . _:b547271955 "2"^^ . _:b547271173 . _:b547271174 . _:b547271436 . _:b547271156 "4"^^ . _:b547271175 . _:b547271224 . _:b547271225 . _:b547271954 "2"^^ . _:b547271226 . _:b547271791 . _:b547271677 . _:b547271439 . _:b547271159 "4"^^ . _:b547271227 . _:b547271228 . _:b547271229 . _:b547271965 "2"^^ . _:b547271460 . _:b547271230 . _:b547271438 . _:b547271158 "4"^^ . _:b547271231 . _:b547271216 . . _:b547271217 . _:b547271969 . _:b547271964 "2"^^ . _:b547271218 . _:b547271433 . . _:b547271153 "4"^^ . . _:b547271219 . _:b547271220 . _:b547271221 . _:b547271967 "2"^^ . _:b547271222 . _:b547271432 . _:b547271152 "4"^^ . _:b547271223 . . . _:b547271208 . _:b547271209 . _:b547271966 "2"^^ . _:b547271210 . _:b547271435 . _:b547271155 "4"^^ . _:b547271211 . _:b547271212 . _:b547271213 . _:b547271961 "2"^^ . _:b547271214 . _:b547271434 . _:b547271354 . _:b547271154 "4"^^ . _:b547271215 . _:b547271200 . . _:b547271201 . _:b547271960 "2"^^ . _:b547271202 . _:b547271619 . _:b547271445 . _:b547271165 "4"^^ . _:b547271203 . _:b547271204 . _:b547271205 . _:b547271963 "2"^^ . _:b547271206 . _:b547271444 . _:b547271164 "4"^^ . . _:b547271207 . _:b547271256 . _:b547271257 . _:b547271962 "2"^^ . _:b547271795 . _:b547271005 . _:b547271447 . _:b547271167 "4"^^ . _:b547271258 . _:b547271259 . _:b547271260 . . _:b547271973 "2"^^ . _:b547271261 . _:b547271262 . _:b547271446 . _:b547271166 "4"^^ . _:b547271263 . _:b547271248 . _:b547271603 . _:b547271249 . _:b547271972 "2"^^ . _:b547271250 . _:b547271441 . _:b547271161 "4"^^ . _:b29539903 _:b29539904 . _:b547271251 . _:b29539903 _:b29539905 . _:b547271252 . _:b29539903 _:b29539906 . _:b547271975 "2"^^ . . _:b547271253 . _:b29539903 _:b29539907 . _:b547271440 . _:b547271160 "4"^^ . _:b547271254 . _:b29539903 _:b29539908 . _:b547271255 . _:b29539903 _:b29539909 . _:b547271240 . _:b547271241 . _:b547271974 "2"^^ . _:b547271676 . _:b547271242 . _:b547271443 . _:b547271163 "4"^^ . _:b547271243 . _:b547271244 . _:b547271245 . _:b547271969 "2"^^ . _:b547271915 . _:b547271246 . _:b547271442 . _:b547271162 "4"^^ . _:b547271247 . _:b547271956 . _:b547271232 . . _:b547271968 "2"^^ . _:b547271953 . _:b547271233 . _:b547271234 . _:b547271453 . _:b547271406 . . _:b547271235 . _:b547271236 . _:b547271237 . _:b547271971 "2"^^ . _:b547271447 . . _:b547271452 . _:b547271238 . _:b547271239 . _:b547271288 . . _:b547271970 "2"^^ . _:b547271289 . _:b547271290 . _:b547271455 . _:b547271291 . _:b547271292 . _:b547271293 . _:b547271981 "2"^^ . _:b547271294 . _:b547271454 . _:b547271295 . _:b547271280 . _:b547271281 . _:b547271980 "2"^^ . _:b547271306 . _:b547271282 . _:b547271449 . _:b547271019 . _:b547271283 . _:b547271284 . _:b547271285 . _:b547271286 . _:b547271448 . . . _:b547271287 . _:b547271272 . . _:b547271273 . _:b547271274 . _:b547271451 . _:b547271275 . _:b547271276 . _:b547271277 . _:b547271977 "2"^^ . _:b547271278 . _:b547271450 . _:b547271365 . _:b547271279 . . _:b547271264 . . _:b547271976 "2"^^ . _:b547271265 . _:b547271266 . _:b547271461 . . _:b547271267 . _:b547271268 . _:b547271269 . _:b547271979 "2"^^ . _:b547271270 . _:b547271460 . _:b547271271 . _:b547271604 . _:b547271576 . _:b547271577 . _:b547271978 "2"^^ . _:b547271119 . _:b547271463 . _:b547271578 . . _:b547271588 . _:b547271579 . _:b547271580 . . _:b547271581 . _:b547271582 . _:b547271462 . _:b547271583 . _:b547271113 . _:b547271568 . _:b547271569 . _:b547271570 . _:b547271657 . _:b547271457 . . _:b547271571 . _:b547271572 . _:b547271573 . _:b547271574 . _:b547271456 . _:b547271575 . _:b547271560 . _:b547271561 . _:b547271562 . _:b547271756 . _:b547271614 . _:b547271459 . _:b547271563 . _:b547271564 . _:b547271565 . _:b547271566 . _:b547271458 . _:b547271567 . _:b547271552 . _:b547271357 . _:b547271553 . _:b547271576 . _:b547271554 . _:b547271469 . . _:b547271555 . _:b547271556 . _:b547271557 . . _:b547271468 . _:b547271558 . _:b547271559 . . _:b547271608 . _:b547271609 . _:b547271610 . _:b547271471 . _:b547271611 . . _:b547271612 . _:b547271613 . _:b547271614 . _:b547271470 . . _:b547271615 . _:b547271600 . _:b547271601 . _:b547271602 . _:b547271465 . _:b547271603 . _:b29539930 . _:b547271349 . . . _:b29539930 "particular those employing multipotent stem cells, may be confounded by unintended mechanical dosing.14 Additionally, stem cells are differentiated, in standard protocols, by delivering specific doses of chemical factors during culture.>>29<< The present findings suggest that an analogous strategy may exist to bias differentiation through the mechanical dosing of stem cells during culture." . _:b29539915 . _:b547271604 . _:b547271605 . _:b547271606 . _:b547271464 . _:b547271607 . _:b547271592 . . _:b547271593 . _:b547271594 . _:b547271467 . _:b547271415 . _:b547271595 . _:b547271596 . _:b547271597 . _:b547271598 . _:b547271466 . _:b547271599 . _:b547271584 . _:b547271314 . _:b547271585 . _:b547271586 . _:b29539912 . _:b547271477 . . _:b547271559 . . _:b547271587 . _:b547271588 . . _:b547271589 . _:b547271590 . _:b547271476 . _:b547271591 . _:b547271640 . _:b29539925 . _:b547271221 . _:b547271641 . _:b547271642 . _:b547271479 . _:b547271643 . . _:b547271644 . . _:b547271645 . _:b547271646 . _:b547271478 . _:b547271647 . _:b547271632 . _:b547271633 . _:b547271634 . _:b547271473 . . . . _:b547271635 . _:b547271636 . _:b547271637 . _:b547271638 . _:b547271472 . _:b547271639 . . _:b547271243 . _:b547271624 . _:b547271625 . _:b547270988 . _:b547271475 . _:b547271626 . _:b547271627 . _:b547271496 . _:b547271628 . _:b547271973 . _:b547271837 . _:b547271629 . _:b547271630 . _:b547271474 . _:b547271631 . . _:b547271616 . _:b547271617 . _:b547271244 . _:b547271485 . . _:b547271618 . . _:b547271081 . _:b547271619 . _:b547271153 . _:b547271400 . _:b547271620 . _:b547271621 . _:b547271622 . _:b547271484 . _:b547271623 . _:b547271672 . _:b547271797 . _:b547271673 . _:b547271674 . _:b547271487 . _:b547271288 . _:b547271675 . _:b547271676 . _:b547271677 . _:b547271912 . _:b547271597 . _:b547271678 . _:b547271486 . _:b547271679 . . _:b547271664 . _:b547271707 . _:b547271080 . _:b547271665 . _:b547271666 . _:b547271481 . _:b547271667 . . _:b547271668 . _:b547271669 . _:b547271670 . _:b547271480 . . _:b547271671 . _:b547271207 . _:b547271656 . . _:b547271657 . _:b547271658 . _:b547271483 . _:b547271659 . . _:b547271660 . _:b547271661 . _:b547271662 . _:b547271482 . . _:b547271663 . _:b547271648 . _:b547271649 . . _:b547271493 . . _:b547271650 . . _:b547270996 . _:b547271651 . . _:b547271652 . . _:b547271653 . . _:b547271492 . _:b547271654 . . _:b547271655 . . _:b547271448 . _:b547271449 . _:b547271450 . _:b547271495 . _:b547271451 . _:b547271452 . _:b29539908 . _:b547271690 . _:b547271453 . _:b547271454 . _:b547271494 . _:b547271455 . _:b547271838 . _:b547271440 . . _:b547271441 . _:b547271442 . _:b547271489 . _:b547271443 . _:b547271444 . _:b547271445 . _:b547271446 . _:b547271488 . _:b547271447 . _:b547271432 . . _:b547271433 . . _:b547271491 . _:b547271434 . _:b547271435 . _:b547271436 . _:b547271437 . _:b547271438 . _:b547271490 . . _:b547271439 . _:b547271424 . _:b547271348 . _:b547271425 . . _:b29539915 . _:b547271947 . _:b547271501 . _:b547271426 . _:b547271427 . _:b547271428 . _:b547271429 . . _:b547271500 . _:b547271430 . _:b547271431 . _:b547271177 . "PMC0" . _:b547271480 . _:b547271481 . _:b547271482 . _:b547271503 . _:b547271483 . _:b547271484 . _:b547271485 . _:b547271486 . _:b547271502 . _:b547271487 . _:b547271472 . _:b547271473 . _:b547271474 . _:b547271497 . _:b547271475 . _:b547271476 . _:b547271477 . _:b547271478 . _:b547271496 . _:b547271479 . . _:b547271340 . _:b547271464 . _:b547271188 . _:b547271465 . . _:b547271499 . _:b547271466 . _:b547271467 . . _:b547271468 . _:b547271469 . _:b547271470 . _:b547271592 . _:b547271498 . _:b547271471 . _:b547271755 . _:b547271003 . _:b547271456 . _:b547271457 . _:b547271458 . _:b547271509 . _:b547271459 . _:b547271460 . _:b547271461 . _:b547271462 . _:b547271508 . _:b547271463 . _:b547271512 . . _:b547271513 . _:b547271514 . _:b547271511 . _:b547271515 . _:b547271516 . _:b547271624 . . _:b547271540 . _:b547271517 . _:b547271518 . _:b547271510 . _:b547271519 . _:b547271504 . _:b547271505 . _:b547271211 . _:b547271505 . _:b547271506 . _:b547271507 . _:b547271508 . _:b547271173 . _:b547271509 . _:b547271510 . _:b547271504 . _:b547271511 . _:b547271496 . _:b547271497 . _:b547271498 . _:b547271507 . . _:b547271499 . _:b547271500 . _:b547271501 . _:b547271667 . _:b547271584 . _:b547271502 . _:b547271506 . _:b547271503 . _:b547271488 . _:b547271805 . _:b547271536 . _:b547271290 . _:b547271489 . _:b547271490 . _:b547271889 . _:b547271517 . _:b547271491 . _:b547271492 . _:b547271493 . _:b547271494 . _:b547271516 . . _:b547271495 . _:b547271544 . _:b547271545 . _:b547271546 . _:b547271519 . _:b547271097 . _:b547271410 . _:b547271547 . _:b547271548 . _:b547271549 . _:b547271550 . _:b547271518 . _:b547271551 . _:b29539915 "conformation, and topography on mechanical memory and dosing? The literature is converging on the paradigm that cellular mechanotransduction is not governed by modulus alone, but through a complex interplay of all of these factors.>>4<<-7 What additional information will be revealed by studying mechanical memory and dosing on substrates with defined and independently varied topography, surface chemistry, protein conformation, and modulus?" . _:b547271536 . _:b547271537 . _:b547271538 . _:b547271513 . _:b547271539 . _:b547271606 . _:b547271540 . _:b547271165 . _:b547271541 . _:b547271007 . _:b547271542 . _:b547271512 . _:b547271543 . _:b547271528 . _:b547271529 . _:b547271530 . _:b29539928 . _:b547271515 . _:b547271531 . _:b547271532 . _:b547271675 . _:b547271533 . _:b547271534 . _:b547271514 . _:b547271535 . . _:b547271520 . _:b547271521 . . _:b547271525 . _:b547271522 . _:b547271523 . _:b547271859 . _:b547271438 . _:b547271524 . _:b547271525 . _:b547271526 . _:b547271524 . _:b547271527 . _:b547271527 . . _:b547271526 . . _:b547271813 . _:b547271521 . _:b29539916 _:b29539917 . _:b29539916 _:b29539918 . _:b29539916 _:b29539919 . _:b547271520 . _:b547271523 . _:b547271257 . _:b547271522 . _:b547271408 . _:b547271533 . _:b547271264 . _:b547271532 . _:b547271976 . _:b547271535 . _:b547271534 . _:b29539910 _:b29539912 . _:b29539910 _:b29539913 . _:b547271732 . _:b547271523 . _:b29539910 _:b29539914 . _:b547271295 . _:b29539910 _:b29539915 . _:b547271529 . _:b29539910 _:b29539911 . _:b547271528 . _:b547271068 . _:b547271531 . . _:b547271530 . _:b547271853 . . _:b547271541 . _:b547271038 . _:b29539917 . _:b547271540 . _:b547271543 . _:b547270998 . _:b547271823 . _:b547271542 . . _:b547271537 . _:b547271833 . _:b547271536 . _:b547271070 . _:b547271539 . _:b29539927 _:b29539928 . _:b29539927 _:b29539929 . _:b29539927 _:b29539930 . _:b547271331 . _:b547271538 . _:b547271549 . _:b547270990 . _:b547271548 . . _:b547271319 . _:b547271551 . . _:b547271950 . _:b547271863 . . _:b547271550 . _:b29539909 . . _:b29539920 _:b29539921 . _:b29539920 _:b29539922 . _:b547271545 . . _:b547271578 . _:b547271661 . _:b547271544 . _:b29539923 _:b29539924 . _:b29539923 _:b29539925 . _:b29539923 _:b29539926 . _:b547271507 . _:b547271547 . _:b547271546 . _:b547271557 . _:b547271620 . . . . _:b547271556 . _:b547271559 . _:b547271763 . . _:b547271147 . _:b547271558 . . _:b547271553 . _:b547271409 . . _:b547271535 . _:b547271552 . . . _:b547271555 . _:b547271554 . _:b547271565 . _:b547271856 . . _:b547271564 . _:b547271567 . . _:b547271566 . . _:b547271561 . _:b547271699 . _:b547271553 . _:b547271560 . . _:b547271729 . . . _:b547271914 . _:b547271563 . . _:b547271562 . _:b547271971 . . _:b547271573 . _:b547271541 . _:b547271572 . . _:b547271575 . _:b547271574 . _:b547271674 . _:b547271006 . . _:b547271341 . . _:b547271569 . . . _:b547271309 . . _:b547271568 . . _:b547271865 . _:b547271571 . . _:b547271570 . _:b547271581 . _:b547271108 . . _:b547271580 . _:b547271960 . _:b547271583 . _:b547271713 . . _:b547271582 . . _:b547271235 . _:b547271577 . . _:b547271160 . _:b547271576 . _:b547271637 . _:b547271721 . . _:b547271897 . . _:b547271579 . . . _:b547271578 . . _:b547271283 . _:b547271589 . _:b547271324 . _:b547271588 . _:b547271591 . _:b547271668 . _:b547271483 . . _:b547271590 . _:b547271585 . . _:b547271584 . . _:b547271587 . _:b547271586 . _:b547271827 . _:b547271545 . _:b547271282 . _:b547271597 . . _:b547271596 . _:b547271548 . _:b547271138 . _:b547271599 . _:b547271205 . _:b547271645 . _:b547271598 . _:b547271720 . _:b547271593 . _:b547271592 . _:b547271946 . _:b547271595 . _:b547271069 . . . _:b547271594 . . _:b547271605 . . _:b547271544 . _:b547271032 . _:b547271604 . _:b547271334 . _:b547271513 . _:b547271607 . _:b547271802 . . . _:b547271606 . _:b547271601 . _:b547271600 . . . _:b547271603 . _:b547271900 . _:b547271602 . _:b547271613 . _:b547271643 . _:b547271612 . _:b547271652 . _:b547271615 . _:b547271963 . . _:b547271216 . . _:b547271614 . . _:b547271609 . _:b547271464 . _:b547271945 . _:b547271078 . . . _:b547271608 . _:b547271927 . _:b547271611 . . _:b547271071 . _:b547271057 . _:b547271610 . . . . _:b547271621 . . _:b547271620 . . _:b547271623 . _:b547271779 . _:b547271622 . _:b547271964 . _:b547271445 . . _:b547271617 . _:b547271616 . _:b547271618 . _:b547271619 . _:b547271618 . _:b547271629 . . _:b547271628 . . _:b547271631 . . _:b547271630 . _:b547271625 . _:b547271878 . . _:b547271624 . _:b547271356 . _:b547271627 . . _:b547271626 . _:b547271105 . . . _:b547271891 . _:b547271637 . _:b547271128 . _:b547271328 . . _:b547271465 . _:b547271636 . _:b547271828 . _:b547271726 . . _:b547271639 . . _:b547271011 . _:b547271638 . . _:b547271633 . . _:b547271066 . _:b547271491 . _:b547271632 . . _:b547271635 . . _:b547271423 . _:b547271634 . _:b547271023 . . _:b547271645 . . _:b547271644 . _:b547271049 . _:b547271647 . _:b547271587 . _:b547271217 . _:b547271646 . . _:b547271641 . . . . _:b547271640 . . _:b547271655 . _:b547271332 . _:b547271643 . _:b547271876 . . . _:b547271642 . . _:b547271393 . . _:b547271966 . . _:b547271653 . . _:b547271577 . . . _:b547271652 . _:b547271655 . . _:b547271654 . . _:b547271226 . . _:b547271649 . _:b547271648 . _:b547271671 . . _:b547271651 . . . _:b547271650 . _:b547271077 . _:b547271090 . . . _:b547271661 . . _:b547271660 . _:b547271928 . . _:b547271663 . _:b547271812 . _:b547271662 . . _:b547271657 . . _:b547271903 . . _:b547271978 . _:b547271656 . . _:b547271659 . _:b547271658 . . _:b547271669 . _:b547271980 . . _:b547271668 . _:b547271671 . _:b547271173 "3"^^ . _:b547271670 . . . _:b547271322 . _:b547271172 "3"^^ . _:b547271665 . _:b547271140 . _:b547271299 . _:b547271175 "3"^^ . _:b547271664 . . _:b547271174 "3"^^ . _:b547271667 . _:b547271759 . . _:b547271666 . _:b547271169 "3"^^ . _:b547271130 . . . _:b547271677 . _:b547271168 "4"^^ . _:b547271484 . _:b547271152 . _:b547271676 . _:b547271171 "3"^^ . _:b547271170 "3"^^ . _:b547271679 . . . _:b547271772 . _:b547271455 . _:b547271181 "3"^^ . _:b547271678 . . _:b547271180 "3"^^ . _:b547271673 . _:b547271433 . _:b29539925 "18-20 PEGdiPDA photodegradable hydrogels enable the user to tune material properties exogenously with precise control21-23 and have been exploited to investigate the cellular response to dynamic alterations in substrate mechanics.>>24<<" . . _:b547271183 "3"^^ . _:b547271672 . . . _:b547271182 "3"^^ . _:b547271675 . _:b547271902 . _:b547271177 "3"^^ . _:b547271674 . _:b547271488 . . _:b547271176 "3"^^ . _:b547271173 . _:b547271040 . _:b547271605 . _:b547271179 "3"^^ . _:b547271172 . _:b547271617 . _:b547271178 "3"^^ . _:b547271611 . _:b547271175 . _:b547271189 "3"^^ . _:b547271174 . . . _:b547271188 "3"^^ . _:b547271169 . _:b547271359 . _:b547271191 "3"^^ . _:b547271168 . . . _:b547271817 . _:b547271190 "3"^^ . _:b547271171 . _:b547271085 . _:b547271877 . _:b547271185 "3"^^ . _:b547271170 . . . _:b547271184 "3"^^ . _:b547271181 . _:b547271186 . _:b547271663 . _:b547271187 "3"^^ . _:b547271180 . _:b547271278 . _:b547271896 . . . _:b547271533 . _:b547271183 . _:b547271186 "3"^^ . _:b547271686 . _:b547271728 . _:b547271197 "3"^^ . _:b547271834 . _:b547271182 . _:b547271355 . _:b547271196 "3"^^ . _:b547271177 . _:b547271146 . _:b547271586 . _:b547271192 . _:b547271199 "3"^^ . _:b547271176 . . _:b547271198 "3"^^ . _:b547271179 . _:b547271195 . . _:b547271193 "3"^^ . _:b547271178 . . _:b547271201 . _:b547271311 . _:b547271192 "3"^^ . _:b547271189 . _:b547271195 "3"^^ . _:b547271188 . . . _:b547271194 "3"^^ . _:b547271191 . _:b547271940 . _:b547271205 "3"^^ . _:b547271190 . _:b547271204 "3"^^ . _:b547271806 . _:b547271185 . _:b547271202 . _:b547271207 "3"^^ . _:b29539925 . _:b547271184 . _:b547271206 "3"^^ . _:b547271187 . . _:b547271201 "3"^^ . _:b547271186 . _:b547271200 "3"^^ . _:b547271197 . . _:b547271377 . _:b547271203 "3"^^ . _:b547271196 . . _:b547271202 "3"^^ . _:b547271199 . . _:b547271213 "3"^^ . _:b547271198 . _:b547271212 "3"^^ . _:b547271193 . . _:b547271215 "3"^^ . _:b547271192 . . _:b547271214 "3"^^ . _:b547271195 . _:b547271297 . . _:b547271687 . _:b547271351 . _:b547271063 . _:b547271209 "3"^^ . _:b547271194 . _:b547271208 "3"^^ . _:b547271754 . _:b547271205 . _:b547271302 . _:b547271211 "3"^^ . _:b547271204 . _:b547271210 "3"^^ . _:b547271207 . . . _:b547271221 "3"^^ . . _:b547271206 . _:b547271443 . _:b547271220 "3"^^ . _:b547271201 . . _:b547271702 . _:b547271223 "3"^^ . _:b547271200 . _:b547271222 "3"^^ . . _:b547271203 . _:b547271227 . . _:b547271217 "3"^^ . _:b547271202 . . . . _:b547271216 "3"^^ . _:b547271213 . . _:b547271219 "3"^^ . _:b547271212 . . _:b547271215 . _:b547271218 "3"^^ . . _:b547271414 . . _:b547271968 . _:b547271634 . _:b547271229 "3"^^ . _:b547271214 . _:b547271904 . _:b547271228 "3"^^ . _:b547271647 . _:b547271437 . _:b547271209 . _:b29539928 "dosing on hMSCs highlight a unique mechanism by which stem cells may integrate and store signals from the ECM. These data also call attention to the manner in which cells are cultured ex vivo (e.g., passage number and substrate modulus).>>17<< Experiments on cell differentiation, cell function, or cell transplantation, in particular those employing multipotent stem cells, may be confounded by unintended mechanical dosing." . _:b547271231 "3"^^ . _:b547271208 . _:b547271230 "3"^^ . _:b547271211 . _:b547271368 . _:b547271399 . _:b547271225 "3"^^ . _:b547271210 . . _:b547271682 . _:b547271224 "3"^^ . _:b547271383 . _:b547271221 . . _:b547271227 "3"^^ . _:b547271220 . _:b547271135 . _:b547271226 "3"^^ . _:b547271590 . _:b547271223 . . . _:b547271222 . . _:b547271237 "3"^^ . _:b547271967 . _:b547271236 "3"^^ . _:b547271217 . . _:b547271256 . _:b547271239 "3"^^ . _:b547271216 . . _:b547271808 . _:b547271238 "3"^^ . _:b547271219 . _:b547271887 . _:b547271233 "3"^^ . _:b547271218 . _:b547271141 . _:b547271232 "3"^^ . _:b547271229 . _:b547271235 "3"^^ . _:b547271228 . _:b547271318 . _:b547271234 "3"^^ . _:b547271231 . _:b547271245 "3"^^ . _:b547271033 . _:b547271230 . _:b547271244 "3"^^ . _:b547271793 . _:b547271225 . _:b547271229 . . _:b547271247 "3"^^ . _:b547271719 . _:b547271372 . _:b547271224 . . . . _:b547271908 . _:b547271452 . _:b547271246 "3"^^ . _:b547271227 . _:b547271241 "3"^^ . _:b547271538 . _:b547271226 . _:b547271913 . _:b547271750 . _:b547271775 . . . _:b547271237 . _:b547271240 "3"^^ . . . _:b547271243 "3"^^ . _:b547271236 . _:b547271214 . . _:b547271242 "3"^^ . _:b547271758 . _:b547271239 . _:b547271803 . _:b547271253 "3"^^ . _:b547271238 . _:b547271252 "3"^^ . _:b547271320 . _:b547271233 . _:b547271255 "3"^^ . _:b547271232 . . . . . _:b547271235 . _:b547271091 . _:b547271254 "3"^^ . _:b547271018 . _:b547271486 . _:b547271249 "3"^^ . _:b547271539 . _:b547271234 . _:b547271248 "3"^^ . _:b547271245 . _:b547271251 "3"^^ . _:b547271244 . . _:b547271250 "3"^^ . _:b547271247 . . . . . _:b547271246 . _:b547271261 "3"^^ . _:b547271169 . _:b547271260 "3"^^ . _:b547271241 . _:b547271263 "3"^^ . _:b547271240 . _:b547271114 . _:b547271262 "3"^^ . _:b547271243 . . _:b547271257 "3"^^ . _:b547271242 . _:b547271199 . _:b547271800 . _:b547271256 "3"^^ . _:b547271253 . . _:b547271259 "3"^^ . _:b547271894 . _:b547271252 . _:b547271258 "3"^^ . _:b547271255 . . _:b547271191 . _:b547271181 . _:b547271254 . _:b547271269 "3"^^ . _:b29539909 "Human mesenchymal stem cells (hMSCs) were isolated from human bone marrow (Lonza) based on their preferential adhesion to tissue culture polystyrene (TCPS) plates.>>31<< Freshly isolated hMSCs were frozen down in 95% fetal bovine serum (FBS; Invitrogen) and 5% DMSO and marked as P1 hMSCs." . _:b547271268 "3"^^ . _:b547271249 . _:b547271271 "3"^^ . _:b547271248 . _:b547271009 . . . _:b547271251 . _:b547271270 "3"^^ . . _:b547271212 . . _:b547271265 "3"^^ . _:b547271250 . _:b547271974 . _:b547271239 . _:b547271264 "3"^^ . . _:b547271261 . _:b29539920 "yap/taz store mechanical information" . _:b547271267 "3"^^ . _:b547271260 . . _:b547271266 "3"^^ . _:b547271263 . _:b547271343 . _:b547271277 "3"^^ . _:b547271262 . _:b547271276 "3"^^ . _:b547271257 . _:b547271749 . _:b547271684 . _:b547271279 "3"^^ . _:b547271310 . _:b547271256 . _:b547271159 . _:b547271278 "3"^^ . _:b547271259 . _:b547271441 . _:b547271171 . . _:b547271273 "3"^^ . _:b547271258 . . _:b547271272 "3"^^ . _:b547271741 . _:b547271269 . _:b547271275 "3"^^ . _:b547271839 . _:b547271268 . _:b547271274 "3"^^ . _:b547271271 . _:b547271285 "3"^^ . _:b547271270 . _:b547271284 "3"^^ . _:b547271265 . _:b547271287 "3"^^ . _:b547271264 . _:b547271286 "3"^^ . _:b547271267 . . . _:b547271281 "3"^^ . _:b547271266 . _:b547271280 "3"^^ . _:b547271277 . _:b547271283 "3"^^ . _:b547271276 . . _:b547271086 . _:b547271282 "3"^^ . _:b547271279 . _:b547271888 . _:b547271293 "3"^^ . _:b547271278 . _:b547271129 . _:b547271292 "3"^^ . _:b547271273 . . . _:b547271272 . _:b547271295 "3"^^ . . . _:b547271294 "3"^^ . _:b547271275 . _:b547271499 . _:b547271048 . _:b547271289 "3"^^ . _:b547271274 . . _:b547271697 . _:b547271288 "3"^^ . _:b547271285 . _:b547271627 . . _:b547271276 . _:b547271291 "3"^^ . _:b547271284 . . _:b29539921 "in the nuclei for DT7 / So3. Additional staining confirmed that TAZ localized with YAP (cytoplasmic on soft substrates and nuclear on stiff substrates); YAP/TAZ together form a functional mechanosensitive entity (Supplementary Fig. S7).>>15<<,16 These findings demonstrate that extended culture on supraphysiologically stiff substrata (TCPS) leads to persistent activation of YAP in hMSCs even after transfer to de-activating, soft hydrogels." . _:b547271670 . . _:b547271290 "3"^^ . _:b547271287 . _:b547271286 . _:b547271301 "3"^^ . _:b547270991 . _:b547271300 "3"^^ . _:b547271281 . . . . _:b547271303 "3"^^ . _:b547271280 . . . _:b547271253 . _:b547271302 "3"^^ . _:b547271283 . . _:b547271282 . _:b547271297 "3"^^ . . _:b547271293 . _:b547271296 "3"^^ . _:b547271203 . _:b547271292 . _:b547271299 "3"^^ . _:b547271295 . _:b547271298 "3"^^ . . _:b547271294 . _:b547271309 "3"^^ . . . _:b547271825 . _:b547271289 . _:b547271308 "3"^^ . _:b547271139 . _:b547271555 . _:b547271288 . _:b547271311 "3"^^ . _:b547271170 . _:b547271848 . _:b547271291 . _:b547271310 "3"^^ . . _:b547271648 . _:b547271290 . _:b547271305 "3"^^ . _:b547271117 . _:b547271857 . . _:b547271301 . _:b547271304 "3"^^ . _:b547271300 . _:b547271307 "3"^^ . _:b547271157 . _:b547271237 . _:b547271306 "3"^^ . _:b547271303 . . _:b547271879 . _:b547271302 . _:b547271317 "3"^^ . _:b547271297 . _:b547271316 "3"^^ . _:b547271327 . _:b547271124 . _:b547271296 . _:b547271319 "3"^^ . . _:b547271884 . _:b547271272 . . _:b547271318 "3"^^ . _:b547271299 . . _:b547271298 . _:b547271313 "3"^^ . "10.1038%2Fnmat3889" . . _:b547271323 . . . _:b547271312 "3"^^ . _:b547271309 . . _:b547271315 "3"^^ . _:b547271308 . _:b547271311 . _:b547271314 "3"^^ . _:b547271474 . . _:b547271310 . _:b547271325 "3"^^ . _:b547271305 . _:b547271324 "3"^^ .