. _:b572937776 . _:b572937836 . _:b572937812 . _:b38191954 "Another arm of this trial \u201CACT II\u201D compares rindopepimut/GM-CSF concurrently with either standard or dose-intensive adjuvant TMZ (>>19<<). All patients were found to have an immune response to EGFRvIII; however, patients in the dose-intensive cohort had an even greater serum response, which may be partly due to a decrease in regulatory T cells (19)." . _:b572937872 . . _:b572937779 . _:b572937939 . _:b572937778 . . _:b572937898 . _:b572937789 . _:b572937539 . . _:b572937788 . . . _:b572937765 . _:b572937791 . . _:b572937859 . _:b572937790 . _:b572937932 . _:b572937785 . _:b38191906 "EGFRvIII cells displayed up-regulation of genes that promote an invasive phenotype such as matrix metalloproteinase (MMP)-13 (>>36<<). In both scratch tests and Matrigel Invasion Chamber assays, the cells also showed greater ability for migration and local tissue invasion than wtEGFR cells (36)." . _:b38191969 . _:b572937784 . _:b572937787 . _:b38191857 . _:b572937830 . . _:b38191971 . _:b572937537 . _:b572937911 . _:b572937786 . "PMC0" . . . _:b38191972 . _:b572937797 . _:b572937870 . _:b572937776 . _:b572937796 . _:b572937799 . . _:b572937798 . . _:b572937793 . . _:b572937792 . . _:b572937795 . _:b572937608 . _:b572937794 . _:b572937771 . . _:b38191962 . _:b572937593 . _:b572937805 . . _:b572937804 . _:b38191867 . _:b572937550 . _:b38191896 "There is also evidence in GBM of EGFRvIII internalization to form an oncogenic complex with STAT3 (>>27<<)." . . _:b572937807 . _:b38191883 "Activation of mTORC1 promotes cellular growth by biosynthesis of proteins, lipids, and organelles in addition to inhibition of catabolic activity (>>22<<). mTORC2 activation results in phosphorylation and subsequent activation of several molecules (including AKT) that are involved in cell survival, metabolism, and proliferation (22). PTEN is a major inhibitor of this pathway by preventing" . . _:b572937806 . _:b38191972 "shown to be an efficacious inhibitor of the EGFR signaling pathway in glioblastoma cell lines in vitro by direct inhibition of the EGFR receptor and further independent down-regulation of AKT, leading to a decrease in cell invasiveness (>>77<<). An increase in the radio-sensitivity of resistant cancer cells has also been described following miR-7 (78)." . . _:b572937801 . _:b572937772 . _:b572937800 . . . . _:b572937803 . . _:b572937802 . _:b572937546 . _:b572937813 . . _:b572937812 . . _:b572937815 . . _:b572937814 . _:b572937576 . _:b572937809 . _:b572937718 . _:b572937853 . _:b572937786 . . _:b572937808 . _:b572937811 . _:b572937810 . . "10.3389%2Ffonc.2015.00005" . _:b572937821 . _:b572937654 . _:b572937820 . _:b38191904 . . . _:b572937684 . _:b572937823 . _:b572937822 . . _:b572937668 . _:b572937817 . _:b38191935 . _:b572937907 . . _:b572937816 . . . . _:b572937819 . _:b572937818 . _:b38191858 . _:b572937829 . _:b572937687 . _:b38191869 . . _:b572937828 . _:b572937763 . . . _:b572937831 . _:b572937801 . _:b572937918 . _:b572937857 . _:b572937830 . . _:b572937851 . _:b572937825 . . . _:b572937824 . . _:b572937658 . _:b572937711 . _:b38191927 . _:b572937827 . _:b38191930 "antibody targeting of egfr" . _:b572937698 . . _:b572937826 . . _:b38191921 "39\u201343, 49)Lapatinib (L01XE07)EGFR/HER1/HER2HER2+ breast cancerResistance to inhibitionAfatinib (L01XE13)EGFR/HER1/HER2/HER4Metastatic NSCLCDacomitinibEGFR/HER1/HER2/HER4N/AVaccinesRindopepimut (CDX-110)EGFRvIIIN/ATumor heterogeneity(>>37<<, 50\u201352)Patient" . . _:b38191912 "As an initial therapy for asymptomatic brain metastases in never-smokers with adenocarcinoma of the lung, the combination of gefitinib and erlotinib has shown response rates of up to 70% (>>43<<). Lapatanib is another tyrosine kinase inhibitor used in treatment of HER2+ breast cancer, which when used in combination with capecitabine was found to increase progression-free survival to 8.4\u2009months compared to 4.4\u2009months receiving" . . _:b572937837 . _:b572937836 . . _:b572937925 . _:b572937839 . _:b38191917 . _:b572937581 . _:b572937524 . _:b572937868 . _:b572937838 . . _:b572937619 . _:b572937833 . _:b572937571 . . _:b572937832 . . . _:b572937835 . _:b38191913 "in combination with capecitabine was found to increase progression-free survival to 8.4\u2009months compared to 4.4\u2009months receiving capecitabine monotherapy at the primary endpoint of a clinical trial of metastatic breast cancer patients (>>44<<). In the monotherapy group, 11 patients had CNS metastases compared with 4 in the combination therapy group (44), though lapatinib has not yet been shown to have activity against recurrent GBM in clinical trials (45)." . _:b572937807 . _:b572937834 . _:b38191960 . . _:b572937845 . . _:b572937951 . _:b572937844 . . _:b572937661 . _:b572937847 . . _:b572937846 . _:b38191868 "This could be due to the large molecular weight of the antibodies reducing their ability to traverse the BBB without assisted transport vectors (>>80<<)." . . _:b572937841 . _:b572937840 . _:b38191918 . _:b572937752 . _:b572937950 . . _:b572937843 . . _:b38191877 "EGFR (also known as HER1 or ERBB1) is a receptor belonging to the ERBB family of the RTKs (>>20<<). Ligand-binding by EGF results in the activation of the RTK/RAS/PI(3)K pathway (21) via receptor phosphorylation, ultimately resulting in cellular proliferation, angiogenesis, and increased local tissue invasion as well as resistance to" . _:b572937842 . . . . _:b572937933 . _:b572937853 . _:b572937521 . . . . _:b572937852 . _:b572937855 . _:b38191900 . _:b572937953 . _:b572937747 . _:b572937854 . . _:b572937538 . _:b38191955 . _:b572937849 . . _:b38191846 . _:b38191954 . _:b572937848 . . _:b38191919 "pancreatic cancerNervous system toxicity125 I-Mab 425EGFRN/AmAb806EGFRvIIIN/ADAB389EGFEGFRN/ASmall molecule inhibitorsGefitinib (L01XE02)EGFR/HER1NSCLCErlotinib (L01XE03)EGFR/HER1NSCLC and pancreatic cancerInsufficient delivery(14\u201317, >>39<<\u201343, 49)Lapatinib (L01XE07)EGFR/HER1/HER2HER2+ breast cancerResistance to inhibitionAfatinib (L01XE13)EGFR/HER1/HER2/HER4Metastatic NSCLCDacomitinibEGFR/HER1/HER2/HER4N/AVaccinesRindopepimut (CDX-110)EGFRvIIIN/ATumor heterogeneity(37," . _:b572937851 . . _:b38191956 . . _:b572937850 . _:b572937649 . _:b572937861 . _:b572937860 . . . _:b38191867 "A further study in rats demonstrated that cetuximab applied by an osmotic mini-pump significantly reduced tumor growth in the brain versus systemic application \u2013 which failed to block tumor growth (>>52<<). This could be due to the large molecular weight of the antibodies reducing their ability to traverse the BBB without assisted transport vectors (80)." . _:b572937738 . _:b572937863 . . _:b38191846 "Recent data from The Cancer Genome Atlas project has proposed various subtypes of GBM, each with distinct molecular properties and genetic aberrations (>>5<<), although there is increasing recognition that there is molecular heterogeneity within individual tumors (6\u20138)." . _:b572937900 . _:b572937862 . _:b572937854 . _:b572937566 . _:b572937575 . _:b572937857 . . _:b38191956 "Most importantly, overall survival was greatly improved (23.6\u2009months) in comparison to historical case-matched controls (>>19<<)." . _:b38191937 . _:b38191854 . . _:b572937856 . _:b38191936 . _:b572937859 . _:b572937858 . _:b38191911 "palliative therapy for patients with brain metastases from NSCLC found that 45% of patients experienced symptom improvement, with the experimental group maintaining progression-free survival for 6\u2009months longer than the control group (>>42<<). As an initial therapy for asymptomatic brain metastases in never-smokers with adenocarcinoma of the lung, the combination of gefitinib and erlotinib has shown response rates of up to 70% (43)." . _:b38191849 . _:b572937837 . . _:b572937869 . . _:b38191880 "Ligand-binding to RTKs simultaneously activates PI3K (see Figure 1) (>>22<<). PI3K phosphorylates phosphatidylinositol 4,5-biphosphate (PIP2) to phosphatidylinositol 3,4,5-triphosphate (PIP3), which results in further activation of AKT (22)." . _:b38191878 . _:b572937868 . . _:b38191877 . _:b572937665 . _:b572937603 . _:b38191879 . _:b572937893 . _:b572937871 . . _:b38191848 . _:b572937870 . . _:b38191949 "immune therapy using vaccines" . _:b572937903 . _:b572937532 . _:b572937865 . . _:b572937864 . _:b38191969 "In pre-clinical studies, ribozymes targeting EGFRvIII were shown to inhibit ERM5\u20131 and U87MG glioblastoma cells (>>71<<, 76). In U87MG.EGFRvIII cells, anti-EGFRvIII hairpin ribozymes resulted in >90% reduction of EGFRvIII mRNA and a reduction in proliferation (71)." . _:b572937867 . . _:b572937709 . . _:b572937866 . . _:b572937549 . _:b572937556 . _:b572937848 . _:b572937877 . _:b572937802 . _:b572937876 . _:b572937579 . _:b572937879 . _:b572937878 . . . _:b572937873 . _:b572937831 . _:b572937757 . _:b572937940 . . _:b572937872 . _:b38191865 "limitations of targeted therapies" . _:b572937875 . _:b572937874 . . _:b38191889 "In vitro studies suggest that the signaling mechanism of EGFRvIII cells can confer resistance to EGFR inhibitors (>>25<<), because EGFRvIII signals via an mTOR2 pathway whereas wtEGFR uses mTOR1 (24)." . _:b38191894 "EGFRvIII is often co-expressed with wtEGFR, typically in tumors with EGFR over-expression, which complicates our understanding of its contribution to tumorigenesis (13, 26, >>27<<). It has been suggested that the transformation happens intracellularly, with EGFRvIII being phosphorylated in an EGFR-dependent manner leading to increased downstream STAT signaling (27)." . _:b38191922 "39\u201343, 49)Lapatinib (L01XE07)EGFR/HER1/HER2HER2+ breast cancerResistance to inhibitionAfatinib (L01XE13)EGFR/HER1/HER2/HER4Metastatic NSCLCDacomitinibEGFR/HER1/HER2/HER4N/AVaccinesRindopepimut (CDX-110)EGFRvIIIN/ATumor heterogeneity(37, >>50<<\u201352)Patient" . . _:b572937885 . . _:b572937884 . _:b572937915 . _:b572937798 . . _:b572937887 . _:b572937559 . _:b572937805 . _:b572937886 . _:b572937582 . _:b572937881 . _:b572937958 . . _:b572937880 . _:b572937883 . . _:b572937858 . _:b572937882 . _:b572937893 . _:b572937892 . _:b572937885 . . _:b572937895 . _:b572937894 . _:b38191882 "AKT then promotes activation of mTOR \u2013 a protein, which exists as two complexes; mTORC1 and mTORC2 (>>24<<). Activation of mTORC1 promotes cellular growth by biosynthesis of proteins, lipids, and organelles in addition to inhibition of catabolic activity (22). mTORC2 activation results in phosphorylation and subsequent activation of several" . _:b38191908 . _:b572937889 . . . _:b572937888 . . _:b572937891 . _:b572937890 . _:b572937954 . . _:b572937901 . . . . _:b572937900 . _:b38191925 "Pre-clinical results demonstrate the ability of TKIs to inhibit tumor cell growth, angiogenesis, survival, and proliferation in several different EGFR transfected GBM cell lines (36, 49, >>53<<, 54). However, these results do not appear to be clinically translatable, as response rates in GBM patients are disappointing for many inhibitors including gefitinib and erlotinib (55, 56)." . . _:b572937903 . _:b572937744 . _:b572937902 . . . . _:b572937897 . _:b572937896 . . _:b572937896 . . . _:b572937733 . _:b572937899 . _:b38191887 "Loss of PTEN, such as the homozygous deletions observed in around 36% of gliomas, results in dramatic up-regulation of this pathway, and may be a major source of resistance to EGFR therapies (5, >>22<<, 23)." . _:b38191921 . _:b572937898 . _:b572937643 . _:b572937909 . _:b572937905 . _:b572937810 . _:b572937908 . . _:b572937911 . . _:b572937910 . _:b572937930 . _:b572937905 . _:b572937904 . . _:b572937907 . . . . _:b572937906 . _:b572937917 . . _:b38191850 "Overall, aberrant amplification, deletion, or mutation of at least one receptor tyrosine kinase (RTK) has been found in 67.3% of GBM, with EGFR accounting for 57.4% (>>10<<) (as shown in Figure 1). In addition, around 50% of patients with EGFR amplification harbor a specific mutation \u2013 known as EGFRvIII \u2013 which results from an in-frame deletion of exons 2\u20137 (11, 12)." . _:b572937916 . . _:b572937808 . _:b572937919 . _:b572937755 . _:b572937918 . _:b572937913 . _:b572937725 . _:b572937912 . . _:b572937557 . . _:b572937915 . _:b38191973 . _:b38191918 . _:b572937914 . _:b572937913 . _:b572937925 . _:b38191922 . _:b572937924 . _:b38191937 "the expression of VEGF in cell supernatant was observed using an enzyme-linked immunosorbent assay, suggesting further potential for application in GBM, as this signaling pathway also contributes to tumor maintenance and angiogenesis (>>59<<)." . _:b572937927 . . . . _:b572937926 . _:b572937766 . _:b38191898 . _:b572937921 . _:b572937569 . . _:b572937678 . _:b38191899 . _:b38191908 "kappa-light-chain-enhancer of activated B cells (NF-\u03BAB) pathway through I\u03BAB\u03B1M gene transfer could play a role in glioma angiogenesis by regulating the expression of vascular endothelial growth factor (VEGF) and interleukin-8 (IL-8) (>>37<<)." . _:b572937901 . _:b572937920 . _:b38191990 "The mechanisms by which intratumoral heterogeneity arises are poorly understood; however, tumor cells have been found to reversibly increase or decrease levels of EGFRvIII expression in order to maximize their growth potential (>>92<<). Erlotinib resistance appears to be linked to EGFRvIII suppression in extrachromosomal DNA in order to successfully evade therapeutic mechanisms that target extrachromosomal oncogenes (92). Surprisingly, in this study, Nathanson et al." . . _:b572937683 . _:b572937611 . _:b572937923 . . . _:b572937843 . . _:b572937922 . _:b572937590 . . . . _:b572937666 . _:b572937933 . . _:b572937932 . . _:b38191991 . _:b572937935 . _:b38191990 . _:b572937931 . _:b572937934 . . . _:b572937522 . _:b38191893 . _:b572937929 . . _:b572937591 . _:b572937928 . . _:b38191968 "These results were reproduced using an intracranial xenograft mouse model, where median overall survival increased by almost 90% (>>40<<)." . _:b38191885 "PTEN is a major inhibitor of this pathway by preventing phosphorylation of PIP2 to PIP3 (>>22<<). Loss of PTEN, such as the homozygous deletions observed in around 36% of gliomas, results in dramatic up-regulation of this pathway, and may be a major source of resistance to EGFR therapies (5, 22, 23)." . _:b572937931 . _:b572937930 . . . _:b572937555 . _:b572937941 . . _:b38191992 . _:b572937940 . _:b572937946 . . . _:b572937707 . _:b572937943 . . _:b572937942 . . _:b572937937 . _:b572937926 . _:b572937936 . . _:b38191929 . _:b572937939 . _:b572937938 . . _:b38191898 . _:b572937828 . _:b572937889 . _:b572937949 . . _:b572937948 . _:b38191888 . _:b572937951 . _:b38191879 . _:b38191970 "In pre-clinical studies, ribozymes targeting EGFRvIII were shown to inhibit ERM5\u20131 and U87MG glioblastoma cells (71, >>76<<). In U87MG.EGFRvIII cells, anti-EGFRvIII hairpin ribozymes resulted in >90% reduction of EGFRvIII mRNA and a reduction in proliferation (71)." . _:b572937950 . _:b38191925 . _:b572937945 . _:b572937604 . . _:b572937944 . . . _:b572937947 . . _:b572937946 . _:b38191898 . _:b572937957 . _:b572937529 . _:b572937716 . _:b572937956 . . . _:b572937600 . _:b38191887 . . _:b572937958 . _:b572937863 . . _:b38191884 . . _:b572937953 . _:b572937733 "2"^^ . _:b572937750 . _:b38191885 . _:b572937952 . _:b572937732 "2"^^ . _:b572937677 . _:b572937616 . . _:b572937955 . . _:b572937735 "2"^^ . _:b38191883 . . _:b572937954 . _:b572937734 "2"^^ . _:b572937842 . _:b38191878 "Ligand-binding by EGF results in the activation of the RTK/RAS/PI(3)K pathway (>>21<<) via receptor phosphorylation, ultimately resulting in cellular proliferation, angiogenesis, and increased local tissue invasion as well as resistance to apoptosis (21\u201323)." . _:b38191880 . . . . _:b38191879 . _:b572937729 "2"^^ . _:b38191881 . _:b38191847 "Genome Atlas project has proposed various subtypes of GBM, each with distinct molecular properties and genetic aberrations (5), although there is increasing recognition that there is molecular heterogeneity within individual tumors (>>6<<\u20138). Primary GBM is frequently associated with epidermal growth factor receptor (EGFR) amplifications compared with secondary GBM, which may arise from lower grade precursors (5, 9)." . . _:b572937728 "2"^^ . . _:b572937720 . _:b572937731 "2"^^ . _:b38191939 "Cetuximab had little effect in both study groups and the median overall survival was 5\u2009months, eliciting no significant correlation between EGFR status and response or overall survival (>>60<<). Other clinical trials involving similar antibody-based therapies have been equally unsuccessful, though a decrease in skin toxicity has been reported with use of nimotuzumab, which could increase its viability as an adjuvant therapy in" . _:b38191985 . _:b572937730 "2"^^ . _:b572937845 . . _:b38191873 . _:b38191886 "Loss of PTEN, such as the homozygous deletions observed in around 36% of gliomas, results in dramatic up-regulation of this pathway, and may be a major source of resistance to EGFR therapies (>>5<<, 22, 23)." . _:b572937741 "2"^^ . . _:b572937740 "2"^^ . _:b572937937 . _:b38191844 . . _:b572937743 "2"^^ . _:b38191845 . _:b572937614 . _:b38191953 . _:b572937742 "2"^^ . _:b38191846 . _:b572937737 "2"^^ . _:b38191847 . _:b572937577 . _:b572937736 "2"^^ . . _:b572937803 . _:b572937739 "2"^^ . _:b38191977 . _:b572937738 "2"^^ . _:b572937749 "2"^^ . . _:b572937748 "2"^^ . _:b38191852 . _:b38191866 "Additionally, at present very little is known about the long-term adverse effects of non-specifically inhibiting EGFR signaling using TKIs, and knowledge of the biological effects of EGFR inhibitors on GBM cells is still incomplete (>>14<<)." . _:b572937751 "2"^^ . _:b38191853 . _:b38191917 "antibodiesCetuximab (L01XC06)EGFR/HER1Colorectal cancerHead and neck cancerPanitumumab (L01XC08)EGFR/HER1Metastatic colorectal cancerCrossing BBBNimotuzumab (L01XC)EGFR/HER1Squamous cell carcinoma of head and neckHypersensitivity(36, >>46<<\u201348)Orphan status for glioma and pancreatic cancerNervous system toxicity125 I-Mab 425EGFRN/AmAb806EGFRvIIIN/ADAB389EGFEGFRN/ASmall molecule inhibitorsGefitinib (L01XE02)EGFR/HER1NSCLCErlotinib (L01XE03)EGFR/HER1NSCLC and pancreatic" . _:b38191910 "survival in non-small cell lung carcinoma (NSCLC) patients, with one meta-analysis reporting 42.9% of patients receiving TKI therapy reaching at least 1\u2009year of progression-free survival compared to 9.7% with chemotherapy (>>41<<). A study of gefitinib as palliative therapy for patients with brain metastases from NSCLC found that 45% of patients experienced symptom improvement, with the experimental group maintaining progression-free survival for 6\u2009months longer" . _:b572937750 "2"^^ . . _:b38191854 . _:b572937595 . _:b572937745 "2"^^ . _:b572937878 . _:b38191855 . _:b572937742 . _:b572937744 "2"^^ . . _:b38191848 . _:b572937623 . . . _:b572937747 "2"^^ . _:b38191971 "EGFRvIII cells, anti-EGFRvIII hairpin ribozymes resulted in >90% reduction of EGFRvIII mRNA and a reduction in proliferation (>>71<<)." . . _:b38191849 . _:b572937541 . _:b572937880 . _:b572937746 "2"^^ . . _:b38191850 . _:b38191899 "This growth advantage is thought to result from an elevated proliferation rate coupled with a reduction in apoptosis (>>28<<). Similar to EGFR signaling, EGFRvIII activates the RTK/RAS/PI3K pathway as a result of EGFRvIII expression (31, 32). This results in increased levels of phosphorylated AKT and reduced levels of P27KIP1, a cell cycle regulator that" . . _:b572937757 "2"^^ . _:b38191851 . _:b572937652 . _:b572937756 "2"^^ . _:b38191860 . _:b572937759 "2"^^ . _:b38191861 . _:b572937758 "2"^^ . _:b38191862 . _:b38191871 . _:b38191897 . _:b572937753 "2"^^ . _:b572937568 . _:b38191863 . _:b572937737 . _:b572937564 . _:b572937752 "2"^^ . _:b572937727 . _:b38191856 . _:b38191858 . _:b38191851 "In addition, around 50% of patients with EGFR amplification harbor a specific mutation \u2013 known as EGFRvIII \u2013 which results from an in-frame deletion of exons 2\u20137 (>>11<<, 12). EGFRvIII can also be present independently of EGFR amplification (13)." . _:b572937755 "2"^^ . . _:b38191857 . _:b38191892 . _:b572937754 "2"^^ . _:b38191858 . _:b38191977 "Loss of PTEN has previously been found to be strongly correlated with treatment failure in GBM (>>87<<), to the extent that the analysis of EGFRvIII and PTEN levels may be used to predict tumor response to TKI therapy (88)." . _:b572937765 "2"^^ . _:b38191859 . . _:b572937764 "2"^^ . _:b38191868 . _:b572937879 . . _:b572937767 "2"^^ . _:b572937777 . _:b38191869 . _:b572937766 "2"^^ . _:b38191947 . _:b38191870 . . _:b572937761 "2"^^ . _:b38191871 . _:b572937753 . _:b572937547 . _:b38191945 . _:b572937760 "2"^^ . _:b38191864 . _:b572937763 "2"^^ . _:b38191865 . _:b572937762 "2"^^ . _:b38191866 . _:b572937773 "2"^^ . . _:b38191892 "EGFRvIII is often co-expressed with wtEGFR, typically in tumors with EGFR over-expression, which complicates our understanding of its contribution to tumorigenesis (>>13<<, 26, 27). It has been suggested that the transformation happens intracellularly, with EGFRvIII being phosphorylated in an EGFR-dependent manner leading to increased downstream STAT signaling (27)." . _:b38191867 . _:b572937772 "2"^^ . _:b572937820 . _:b572937721 . . _:b572937775 "2"^^ . . _:b572937774 "2"^^ . . _:b572937673 . . _:b572937769 "2"^^ . _:b572937825 . _:b572937768 "2"^^ . _:b572937784 . _:b38191853 "EGFRvIII can also be present independently of EGFR amplification (>>13<<)." . _:b572937771 "2"^^ . _:b38191922 . _:b572937770 "2"^^ . _:b572937781 "2"^^ . _:b572937780 "2"^^ . _:b572937916 . . _:b572937783 "2"^^ . _:b572937856 . . . _:b572937782 "2"^^ . . . _:b572937826 . _:b572937756 . _:b572937777 "2"^^ . _:b38191965 "In addition, in a U251 subcutaneous mouse model treated with antisense RNA and siRNA had significantly smaller tumor volumes by 29 and 19%, respectively, when compared to controls, further demonstrating efficacy in vivo (>>40<<)." . . . _:b572937650 . _:b572937776 "2"^^ . _:b572937586 . _:b572937779 "2"^^ . . _:b38191980 "Additionally, in GBM there is a redundancy in activation of PI3K due to the availability of several types of tyrosine kinases upstream (86), including GFR1, MET, PDGFR\u03B1/\u03B2, and uPAR (>>89<<, 90). Increased activation of other members of the ERBB family of tyrosine kinases has also been described, as compensatory activation of ERBB2 and ERBB3 was noted after EGFR withdrawal in GBM cancer-stem-cell lines (91)." . _:b38191918 . _:b572937778 "2"^^ . . _:b572937789 "2"^^ . . _:b572937788 "2"^^ . . _:b38191953 "It is important to note that positive results in these clinical trials could be due at least in part to the use of GM-CSF, as its use in cancer immunotherapy is enhancement of immunotherapeutic mechanisms of tumor destruction (>>52<<)." . _:b572937795 . _:b572937692 . _:b572937791 "2"^^ . _:b38191893 "EGFRvIII is often co-expressed with wtEGFR, typically in tumors with EGFR over-expression, which complicates our understanding of its contribution to tumorigenesis (13, >>26<<, 27). It has been suggested that the transformation happens intracellularly, with EGFRvIII being phosphorylated in an EGFR-dependent manner leading to increased downstream STAT signaling (27)." . _:b572937920 . . _:b572937790 "2"^^ . . _:b572937785 "2"^^ . . _:b572937784 "2"^^ . _:b572937699 . . _:b572937618 . . _:b572937700 . . . _:b572937787 "2"^^ . . _:b572937786 "2"^^ . _:b572937921 . _:b572937797 "2"^^ . _:b38191886 . _:b572937542 . _:b572937796 "2"^^ . _:b572937583 . . . _:b572937799 "2"^^ . _:b572937798 "2"^^ . _:b572937517 . . . . _:b572937793 "2"^^ . _:b572937792 . . _:b38191856 "Due to the frequency of EGFR aberrations, many EGFR-targeted therapies are in development or clinical trials (>>14<<). Although EGFR kinase inhibitor therapy has shown initial success in other cancers such non-small cell lung cancer (15), previous trials in glioblastoma have been unsuccessful to date (16\u201318)." . _:b38191919 . . _:b572937792 "2"^^ . _:b572937519 . . _:b572937795 "2"^^ . _:b572937783 . _:b572937518 . . _:b572937794 "2"^^ . . . _:b572937526 . _:b572937761 . _:b38191905 . _:b38191912 . _:b572937805 "2"^^ . _:b572937804 "2"^^ . . _:b572937807 "2"^^ . _:b572937809 . _:b572937806 "2"^^ . _:b38191957 "Also, at Stanford University a Phase I trial is underway utilizing the EGFRvIII vaccine in children with diffuse intrinsic pontine gliomas, as EGFR expression has been found to occur in ~50% of the tumors studied (>>67<<)." . _:b572937525 . _:b572937801 "2"^^ . . . _:b572937890 . _:b572937816 . _:b572937524 . . _:b572937800 "2"^^ . _:b572937527 . _:b572937686 . _:b572937803 "2"^^ . . . . _:b572937526 . _:b572937802 "2"^^ . . _:b38191871 "In RNA studies, AAV/shRNA vectors were found to be severely toxic, and caused fatality in 64% of mice due to oversaturation of RNAi pathways (>>82<<, 83). Furthermore, RNA entities are anionic, hydrophilic, and unable to enter cell by passive diffusion mechanisms, so the BBB is essentially impenetrable to any potentially therapeutic RNA molecules (84)." . _:b572937941 . _:b572937521 . _:b572937813 "2"^^ . _:b572937520 . . _:b572937945 . _:b572937812 "2"^^ . _:b572937523 . _:b572937815 "2"^^ . _:b572937829 . _:b572937522 . . _:b572937814 "2"^^ . . . _:b572937533 . _:b572937862 . _:b572937809 "2"^^ . _:b572937532 . _:b572937808 "2"^^ . _:b38191964 . . _:b38191919 . _:b572937535 . _:b572937811 "2"^^ . . _:b572937534 . . _:b38191948 . _:b572937810 "2"^^ . _:b572937715 . . _:b572937529 . _:b572937821 "2"^^ . _:b572937528 . _:b572937820 "2"^^ . _:b572937531 . _:b572937689 . _:b572937823 "2"^^ . _:b572937530 . _:b572937675 . _:b572937822 "2"^^ . _:b38191943 "Antibodies, which utilize toxins or radioisotopes, could provide a potent adjuvant therapy for GBM as they enhance cell killing by the immune system in addition to inhibition of EGFR signaling (>>61<<). Various early clinical trials report that administering the radiolabeled antibody 125I-MAb 425 intravenously, either alone or with standard of care treatment, significantly improves median survival (38, 50, 61)." . . _:b572937541 . _:b572937902 . _:b572937817 "2"^^ . _:b572937540 . _:b572937816 "2"^^ . _:b572937834 . _:b38191865 _:b38191872 . _:b38191865 _:b38191873 . _:b38191865 _:b38191874 . _:b572937892 . _:b572937789 . _:b572937543 . _:b38191865 _:b38191875 . _:b572937819 "2"^^ . _:b572937641 . _:b38191858 "Although EGFR kinase inhibitor therapy has shown initial success in other cancers such non-small cell lung cancer (15), previous trials in glioblastoma have been unsuccessful to date (>>16<<\u201318). More recently, interest has focused on an anti-EGFRvIII vaccine (known as rindopepimut), which has entered clinical trials (19)." . _:b572937542 . _:b572937818 "2"^^ . _:b38191920 "cancerNervous system toxicity125 I-Mab 425EGFRN/AmAb806EGFRvIIIN/ADAB389EGFEGFRN/ASmall molecule inhibitorsGefitinib (L01XE02)EGFR/HER1NSCLCErlotinib (L01XE03)EGFR/HER1NSCLC and pancreatic cancerInsufficient delivery(14\u201317, 39\u201343, >>49<<)Lapatinib (L01XE07)EGFR/HER1/HER2HER2+ breast cancerResistance to inhibitionAfatinib (L01XE13)EGFR/HER1/HER2/HER4Metastatic NSCLCDacomitinibEGFR/HER1/HER2/HER4N/AVaccinesRindopepimut (CDX-110)EGFRvIIIN/ATumor heterogeneity(37," . . . _:b572937537 . _:b572937829 "2"^^ . _:b572937536 . _:b572937828 "2"^^ . _:b572937539 . _:b572937831 "2"^^ . _:b572937538 . _:b572937830 "2"^^ . _:b572937549 . _:b572937645 . _:b572937621 . _:b572937825 "2"^^ . _:b572937548 . _:b572937695 . _:b572937824 "2"^^ . _:b38191858 . . _:b572937601 . _:b572937551 . _:b572937827 "2"^^ . _:b572937550 . _:b572937826 "2"^^ . _:b38191958 "pre-clinical and clinical trials of rna-based therapies" . _:b572937710 . _:b572937545 . _:b572937703 . . _:b572937837 "2"^^ . _:b572937520 . _:b572937544 . _:b572937796 . _:b572937836 "2"^^ . _:b572937547 . . _:b572937839 "2"^^ . _:b38191875 "Furthermore, not all of the samples with genetic amplifications also had increased levels of RNA, which further complicates the assessment of the viability of using EGFR-targeted therapies (>>85<<)." . _:b572937546 . . _:b572937838 "2"^^ . _:b572937723 . _:b572937557 . _:b572937804 . _:b572937833 "2"^^ . . _:b38191920 . _:b572937732 . _:b572937556 . . _:b572937832 "2"^^ . _:b38191986 . . . . _:b572937559 . _:b572937835 "2"^^ . . _:b572937552 . . . _:b572937714 . _:b572937558 . . _:b572937834 "2"^^ . . _:b572937553 . _:b572937845 "2"^^ . _:b572937917 . . _:b38191980 . _:b38191924 . _:b572937552 . _:b572937704 . _:b572937923 . _:b572937844 "2"^^ . _:b572937555 . _:b572937874 . _:b572937682 . _:b572937847 "2"^^ . _:b572937554 . _:b572937823 . _:b572937846 "2"^^ . _:b38191988 . _:b572937717 . _:b572937749 . _:b572937565 . . _:b572937841 "2"^^ . _:b38191911 . . _:b572937793 . _:b572937681 . _:b572937564 . . _:b572937840 "2"^^ . _:b38191915 . _:b572937587 . _:b572937567 . . _:b572937843 "2"^^ . _:b572937566 . _:b572937657 . _:b572937842 "2"^^ . . _:b572937561 . _:b572937773 . _:b572937605 . _:b572937853 "2"^^ . . . _:b572937560 . _:b572937852 "2"^^ . . _:b572937894 . . _:b572937799 . _:b572937563 . _:b572937855 "2"^^ . _:b38191931 "Despite the success of antibody-based therapy in the treatment of renal cell carcinoma, melanoma, and hematologic cancers, these results have not been replicated in GBM (>>46<<, 57, 58). Conjugated and unconjugated antibodies have been developed to target both wtEGFR and EGFRvIII, the most successful so far being cetuximab, panitumumab, and nimotuzumab (41, 47)." . . . _:b572937797 . _:b572937562 . . _:b572937854 "2"^^ . _:b572937606 . . _:b572937573 . . _:b38191919 . _:b572937849 "2"^^ . _:b572937572 . . _:b38191955 "All patients were found to have an immune response to EGFRvIII; however, patients in the dose-intensive cohort had an even greater serum response, which may be partly due to a decrease in regulatory T cells (>>19<<). Most importantly, overall survival was greatly improved (23.6\u2009months) in comparison to historical case-matched controls (19)." . _:b572937848 "2"^^ . _:b572937745 . _:b572937575 . _:b572937851 "2"^^ . _:b572937574 . _:b38191850 . _:b572937850 "2"^^ . . _:b572937569 . _:b38191963 . _:b572937861 "2"^^ . . . _:b572937568 . _:b572937754 . _:b572937860 "2"^^ . _:b572937814 . _:b572937571 . _:b572937863 "2"^^ . . _:b572937570 . _:b572937862 "2"^^ . _:b38191914 "In the monotherapy group, 11 patients had CNS metastases compared with 4 in the combination therapy group (>>44<<), though lapatinib has not yet been shown to have activity against recurrent GBM in clinical trials (45)." . . _:b572937596 . _:b572937791 . _:b572937581 . _:b572937857 "2"^^ . . . . _:b572937580 . . . _:b38191978 . _:b572937856 "2"^^ . _:b572937908 . _:b38191916 "antibodiesCetuximab (L01XC06)EGFR/HER1Colorectal cancerHead and neck cancerPanitumumab (L01XC08)EGFR/HER1Metastatic colorectal cancerCrossing BBBNimotuzumab (L01XC)EGFR/HER1Squamous cell carcinoma of head and neckHypersensitivity(>>36<<, 46\u201348)Orphan status for glioma and pancreatic cancerNervous system toxicity125 I-Mab 425EGFRN/AmAb806EGFRvIIIN/ADAB389EGFEGFRN/ASmall molecule inhibitorsGefitinib (L01XE02)EGFR/HER1NSCLCErlotinib (L01XE03)EGFR/HER1NSCLC and pancreatic" . . _:b572937583 . . _:b572937859 "2"^^ . _:b572937631 . _:b572937582 . _:b572937858 "2"^^ . _:b572937577 . _:b38191960 "The use of antisense oligonucleotides to inhibit translation of mRNA has already yielded good results in pre-clinical studies for NSCLC and prostate cancer (68, >>69<<). Following this, several experimental RNA methods targeting EGFR and EGFRvIII have been developed, including antisense oligonucleotides, RNA interference (RNAi), and ribozymes (40, 70, 71)." . . . _:b572937869 "2"^^ . . . _:b572937576 . _:b572937868 "2"^^ . . _:b572937579 . _:b572937909 . _:b572937762 . _:b572937871 "2"^^ . _:b38191951 "Patients were also found to be immunologically responsive when their cellular immune responses were tested regularly using skin tests (>>51<<). Histological analysis in recurrent GBM patients who received the same vaccine showed no residual expression of EGFRvIII, demonstrating that the vaccine can effectively eliminate EGFRvIII cells, though all other cell types remain intact" . _:b572937865 . _:b572937578 . _:b572937870 "2"^^ . . . _:b38191862 "Antibody-based therapy uses monoclonal antibodies that correspond to the receptor landscape to inhibit signaling, and can also use conjugated antibodies that allow toxins or radioactive isotopes to be targeted to specific cells (>>38<<). The current immunotherapy for EGFRvIII can be administered in the form of an intradermal vaccine CDX-110 and granulocyte macrophage-colony stimulating factor (GM-CSF) (39). RNA therapies will also be discussed, which involve creating" . _:b572937589 . _:b572937865 "2"^^ . _:b572937935 . _:b572937588 . _:b572937741 . _:b572937671 . _:b572937864 "2"^^ . . . _:b572937770 . _:b572937719 . _:b572937591 . _:b572937867 "2"^^ . _:b572937590 . . _:b572937866 "2"^^ . _:b572937609 . _:b572937563 . _:b572937585 . _:b572937877 "2"^^ . . _:b38191876 . _:b38191932 "Despite the success of antibody-based therapy in the treatment of renal cell carcinoma, melanoma, and hematologic cancers, these results have not been replicated in GBM (46, 57, >>58<<). Conjugated and unconjugated antibodies have been developed to target both wtEGFR and EGFRvIII, the most successful so far being cetuximab, panitumumab, and nimotuzumab (41, 47)." . _:b572937584 . _:b572937876 "2"^^ . _:b38191877 . _:b572937587 . _:b572937540 . _:b38191878 . _:b572937879 "2"^^ . _:b572937861 . _:b38191922 . _:b572937586 . _:b38191959 . _:b572937873 . _:b38191879 . _:b572937878 "2"^^ . . _:b572937768 . _:b572937558 . . . _:b572937912 . _:b572937597 . _:b572937873 "2"^^ . _:b38191872 . _:b572937886 . _:b572937782 . _:b572937596 . . _:b38191873 . _:b572937872 "2"^^ . _:b38191864 . _:b38191944 "Various early clinical trials report that administering the radiolabeled antibody 125I-MAb 425 intravenously, either alone or with standard of care treatment, significantly improves median survival (>>38<<, 50, 61). In the largest Phase II trial to date combined treatment of 125I-mAb 425 and TMZ provided the greatest survival benefit with a median survival of 20.4\u2009months, compared to treatment of 125I-mAb 425 alone, which was 14.5\u2009months" . . _:b572937599 . _:b572937875 "2"^^ . _:b38191874 . . _:b572937598 . _:b572937874 "2"^^ . _:b38191875 . _:b572937956 . _:b572937593 . _:b572937928 . _:b572937885 "2"^^ . _:b38191884 . _:b572937860 . _:b572937592 . _:b572937884 "2"^^ . _:b38191885 . _:b572937602 . _:b572937595 . _:b572937561 . _:b572937887 "2"^^ . _:b38191886 . . _:b572937594 . _:b572937560 . _:b38191887 . _:b572937886 "2"^^ . _:b38191855 . _:b572937605 . _:b572937881 "2"^^ . _:b38191880 . _:b38191944 . _:b572937604 . _:b572937880 "2"^^ . _:b38191881 . _:b572937607 . _:b572937883 "2"^^ . _:b38191882 . . . . . _:b572937606 . _:b572937882 "2"^^ . _:b38191883 . _:b572937944 . _:b38191936 "Treatment of EGFR-amplified GBM cells with cetuximab in subcutaneous and intracranial mouse xenografts has been found to result in a significant decrease in proliferation, and an increase in overall survival as well as apoptosis (>>59<<). A decrease in the expression of VEGF in cell supernatant was observed using an enzyme-linked immunosorbent assay, suggesting further potential for application in GBM, as this signaling pathway also contributes to tumor maintenance and" . _:b572937601 . _:b572937893 "2"^^ . _:b38191892 . . . _:b38191959 "The use of antisense oligonucleotides to inhibit translation of mRNA has already yielded good results in pre-clinical studies for NSCLC and prostate cancer (>>68<<, 69). Following this, several experimental RNA methods targeting EGFR and EGFRvIII have been developed, including antisense oligonucleotides, RNA interference (RNAi), and ribozymes (40, 70, 71)." . _:b572937600 . _:b572937892 "2"^^ . _:b38191893 . . . _:b572937603 . . . _:b572937895 "2"^^ . _:b38191894 . . . _:b572937602 . _:b572937589 . _:b38191895 . _:b572937894 "2"^^ . _:b572937735 . . . _:b572937613 . _:b572937889 "2"^^ . _:b38191923 . _:b38191888 . _:b38191981 . _:b572937612 . _:b572937888 "2"^^ . _:b38191889 . . _:b38191861 . . _:b572937615 . _:b572937846 . _:b572937588 . _:b38191890 . _:b572937891 "2"^^ . . _:b38191987 "expression of other ERBB receptors may render EGFR signaling dispensable in the tumor, allowing growth and survival without EGFR signaling and thus negating the therapeutic viability of EGFR-targeting therapies in these cases (87, 88, >>91<<). This uncoupling of the downstream pathways from EGFR signaling could be a possible explanation for the poor clinical responses exhibited with TKIs such as erlotinib (88)." . _:b572937614 . _:b572937890 "2"^^ . . _:b38191891 . _:b572937609 . _:b38191964 "Injection of vectors containing antisense RNA to target EGFRvIII into intracranial glioblastoma xenografts were found to reduce tumor volume by >40-fold compared with controls (>>74<<). In addition, in a U251 subcutaneous mouse model treated with antisense RNA and siRNA had significantly smaller tumor volumes by 29 and 19%, respectively, when compared to controls, further demonstrating efficacy in vivo (40)." . _:b572937901 "2"^^ . _:b38191900 . _:b38191856 . _:b572937608 . _:b38191974 "However, development of miRNA-based therapies against glioma may be considerably more difficult due to the lack of a delivery system sufficient to bypass the blood\u2013brain barrier (>>79<<)." . _:b572937927 . _:b572937900 "2"^^ . _:b38191901 . . _:b572937611 . _:b572937903 "2"^^ . _:b38191902 . _:b572937533 . _:b572937610 . _:b572937902 "2"^^ . _:b38191903 . . _:b572937626 . . _:b572937621 . _:b38191993 . _:b572937897 "2"^^ . _:b38191896 . _:b38191952 "Histological analysis in recurrent GBM patients who received the same vaccine showed no residual expression of EGFRvIII, demonstrating that the vaccine can effectively eliminate EGFRvIII cells, though all other cell types remain intact (>>63<<)." . . _:b572937620 . . _:b38191897 . _:b572937896 "2"^^ . _:b572937623 . _:b572937844 . _:b38191898 . _:b572937899 "2"^^ . . _:b38191866 . . . _:b572937622 . _:b572937898 "2"^^ . _:b38191899 . _:b572937899 . _:b38191982 "Increased activation of other members of the ERBB family of tyrosine kinases has also been described, as compensatory activation of ERBB2 and ERBB3 was noted after EGFR withdrawal in GBM cancer-stem-cell lines (>>91<<). This means that even after totally depleting EGFRvIII expressing populations of cells specifically, other tumor sub-clones with alternative mutations could be selected for, maintaining the tumor population and allowing functional" . _:b38191870 "One hypothesis is that a small tumor may still be relatively immunoprivileged; thus, the immune system may be relatively na\u00EFve to tumor antigens (>>39<<)." . . _:b572937617 . _:b572937909 "2"^^ . _:b38191908 . _:b572937616 . _:b572937881 . _:b572937908 "2"^^ . . _:b38191909 . _:b572937619 . _:b572937911 "2"^^ . _:b38191910 . _:b38191933 . . . _:b572937618 . . _:b38191916 . _:b38191911 . _:b572937910 "2"^^ . _:b572937528 . _:b572937629 . _:b572937905 "2"^^ . _:b38191907 . _:b38191904 . _:b572937628 . . _:b38191906 . _:b38191905 . _:b572937904 "2"^^ . . _:b572937631 . _:b572937907 "2"^^ . _:b38191906 . _:b572937599 . _:b572937597 . _:b572937630 . _:b572937906 "2"^^ . _:b38191907 . _:b572937787 . _:b38191945 "Various early clinical trials report that administering the radiolabeled antibody 125I-MAb 425 intravenously, either alone or with standard of care treatment, significantly improves median survival (38, >>50<<, 61). In the largest Phase II trial to date combined treatment of 125I-mAb 425 and TMZ provided the greatest survival benefit with a median survival of 20.4\u2009months, compared to treatment of 125I-mAb 425 alone, which was 14.5\u2009months (50)." . _:b572937543 . _:b572937625 . _:b38191845 "There is an urgent need for new molecular targeted therapies for newly diagnosed GBM (>>1<<\u20134). Recent data from The Cancer Genome Atlas project has proposed various subtypes of GBM, each with distinct molecular properties and genetic aberrations (5), although there is increasing recognition that there is molecular heterogeneity" . _:b572937778 . _:b572937917 "2"^^ . _:b38191916 . _:b38191961 "Following this, several experimental RNA methods targeting EGFR and EGFRvIII have been developed, including antisense oligonucleotides, RNA interference (RNAi), and ribozymes (>>40<<, 70, 71)." . _:b572937624 . _:b38191979 . _:b572937916 "2"^^ . _:b38191917 . _:b572937627 . _:b38191976 . _:b572937659 . _:b572937919 "2"^^ . _:b38191918 . . _:b572937626 . _:b572937918 "2"^^ . _:b38191919 . _:b572937637 . _:b572937869 . _:b572937913 "2"^^ . _:b38191912 . _:b572937938 . . _:b38191983 . _:b572937636 . _:b572937912 "2"^^ . _:b38191913 . _:b38191966 "Therapy with siRNA leads to post-transcriptional gene silencing that results in the destruction of the target mRNA (>>75<<). siRNA against EGFR has caused up to 90% knockdown of EGFR mRNA in U251 glioma cells (40)." . . _:b572937639 . _:b572937915 "2"^^ . _:b38191914 . _:b38191844 _:b38191856 . _:b38191844 _:b38191857 . _:b38191844 _:b38191858 . _:b572937638 . _:b38191844 _:b38191859 . _:b38191915 . _:b572937914 "2"^^ . . _:b572937633 . _:b572937925 "2"^^ . _:b38191924 . . _:b572937728 . _:b572937632 . _:b38191851 . _:b572937924 "2"^^ . _:b38191925 . _:b38191844 _:b38191845 . _:b38191874 . _:b38191844 _:b38191846 . _:b572937635 . _:b38191844 _:b38191847 . _:b38191926 . _:b572937927 "2"^^ . . _:b38191875 . _:b572937731 . _:b572937633 . _:b572937634 . _:b572937926 "2"^^ . _:b38191927 . _:b38191844 _:b38191852 . _:b38191844 _:b38191853 . _:b38191844 _:b38191854 . _:b572937645 . . _:b38191844 _:b38191855 . _:b38191920 . _:b572937921 "2"^^ . _:b38191844 _:b38191848 . _:b38191919 . _:b572937867 . _:b38191844 _:b38191849 . _:b38191844 _:b38191850 . _:b572937644 . _:b38191844 _:b38191851 . _:b38191921 . _:b572937920 "2"^^ . _:b38191951 . _:b572937647 . _:b572937824 . _:b38191922 . _:b572937923 "2"^^ . _:b38191882 . _:b38191907 "In both scratch tests and Matrigel Invasion Chamber assays, the cells also showed greater ability for migration and local tissue invasion than wtEGFR cells (>>36<<)." . _:b38191950 . . _:b572937646 . . _:b572937922 "2"^^ . _:b38191923 . _:b572937518 "15"^^ . . _:b572937641 . _:b572937933 "2"^^ . _:b38191932 . _:b572937519 "14"^^ . _:b572937517 "24"^^ . _:b572937640 . _:b572937932 "2"^^ . _:b38191933 . _:b38191928 . _:b572937520 "14"^^ . _:b572937526 "7"^^ . . _:b572937643 . _:b572937935 "2"^^ . _:b38191934 . _:b572937562 . _:b572937527 "7"^^ . _:b572937642 . _:b572937647 . _:b38191935 . _:b572937934 "2"^^ . _:b38191910 . _:b572937524 "7"^^ . _:b572937653 . _:b38191979 "Additionally, in GBM there is a redundancy in activation of PI3K due to the availability of several types of tyrosine kinases upstream (>>86<<), including GFR1, MET, PDGFR\u03B1/\u03B2, and uPAR (89, 90)." . _:b572937780 . _:b572937929 "2"^^ . _:b38191928 . _:b38191962 "Following this, several experimental RNA methods targeting EGFR and EGFRvIII have been developed, including antisense oligonucleotides, RNA interference (RNAi), and ribozymes (40, 70, >>71<<)." . _:b572937525 "7"^^ . _:b572937652 . _:b572937655 . _:b572937928 "2"^^ . _:b38191929 . _:b38191860 _:b38191861 . . _:b572937522 "8"^^ . _:b572937655 . . _:b38191860 _:b38191862 . _:b38191860 _:b38191863 . _:b572937931 "2"^^ . _:b572937635 . _:b38191930 . _:b38191890 . . _:b38191847 . _:b572937523 "8"^^ . _:b38191901 . _:b572937654 . _:b572937930 "2"^^ . _:b38191931 . _:b572937521 "10"^^ . _:b572937694 . _:b572937680 . . . _:b572937578 . . _:b572937649 . . _:b38191940 . _:b572937941 "2"^^ . . _:b38191860 _:b38191864 . . _:b572937648 . _:b572937940 "2"^^ . _:b38191941 . _:b572937534 "6"^^ . _:b572937651 . . _:b38191942 . _:b572937943 "2"^^ . _:b572937535 "6"^^ . _:b38191933 "Conjugated and unconjugated antibodies have been developed to target both wtEGFR and EGFRvIII, the most successful so far being cetuximab, panitumumab, and nimotuzumab (>>41<<, 47). The unconjugated antibodies bind the extracellular domain of EGFR, and they are also suggested to cause internalization of EGFRvIII, though clinical trials have had varying results (48)." . _:b572937650 . _:b572937942 "2"^^ . _:b38191943 . _:b572937532 "6"^^ . . _:b572937661 . _:b572937937 "2"^^ . _:b38191970 . _:b38191936 . _:b572937533 "6"^^ . _:b38191918 "and pancreatic cancerNervous system toxicity125 I-Mab 425EGFRN/AmAb806EGFRvIIIN/ADAB389EGFEGFRN/ASmall molecule inhibitorsGefitinib (L01XE02)EGFR/HER1NSCLCErlotinib (L01XE03)EGFR/HER1NSCLC and pancreatic cancerInsufficient delivery(>>14<<\u201317, 39\u201343, 49)Lapatinib (L01XE07)EGFR/HER1/HER2HER2+ breast cancerResistance to inhibitionAfatinib (L01XE13)EGFR/HER1/HER2/HER4Metastatic NSCLCDacomitinibEGFR/HER1/HER2/HER4N/AVaccinesRindopepimut (CDX-110)EGFRvIIIN/ATumor" . _:b572937660 . _:b572937936 "2"^^ . _:b38191937 . _:b572937530 "7"^^ . _:b572937663 . _:b572937939 "2"^^ . _:b38191938 . _:b572937531 "7"^^ . _:b572937662 . _:b572937938 "2"^^ . _:b38191939 . _:b572937528 "7"^^ . _:b38191967 "siRNA against EGFR has caused up to 90% knockdown of EGFR mRNA in U251 glioma cells (>>40<<). These results were reproduced using an intracranial xenograft mouse model, where median overall survival increased by almost 90% (40)." . . _:b572937657 . _:b38191989 "heterogeneity found that it can be maintained by interactions between tumor cells, including the up-regulation of IL-6 production in EGFRvIII cells to activate neighboring wtEGFR cells, enhancing tumor growth and resistance to therapy (>>7<<). The mechanisms by which intratumoral heterogeneity arises are poorly understood; however, tumor cells have been found to reversibly increase or decrease levels of EGFRvIII expression in order to maximize their growth potential (92)." . _:b572937949 "2"^^ . _:b38191948 . _:b38191868 . _:b572937529 "7"^^ . _:b38191973 "An increase in the radio-sensitivity of resistant cancer cells has also been described following miR-7 (>>78<<). The first miRNA-based cancer therapy (MRX34) has recently entered a Phase I clinical trial to evaluate its safety for use against primary liver cancer and liver metastases (NCT01829971). However, development of miRNA-based therapies" . _:b572937706 . _:b38191865 . _:b572937656 . . _:b572937948 "2"^^ . _:b38191949 . . _:b572937659 . . _:b572937541 "5"^^ . _:b38191950 . _:b572937951 "2"^^ . . _:b38191902 "This results in increased levels of phosphorylated AKT and reduced levels of P27KIP1, a cell cycle regulator that inhibits G1\u2013S phase transition in cell lines (>>33<<). Furthermore, abnormal spindle-like microcephaly-associated (ASPM) protein expression has been described in the U87MG.EGFRvIII cells compared to parental U87MG cells (34). ASPM promotes neural stem cell self-proliferation and hence it" . _:b572937658 . _:b572937540 "5"^^ . _:b38191951 . _:b572937950 "2"^^ . _:b38191860 . . _:b572937543 "4"^^ . _:b572937669 . _:b38191865 _:b38191866 . _:b38191865 _:b38191867 . _:b38191944 . _:b572937945 "2"^^ . _:b38191865 _:b38191868 . _:b572937565 . _:b38191865 _:b38191869 . _:b572937607 . _:b572937542 "4"^^ . _:b572937668 . _:b38191865 _:b38191870 . _:b38191865 _:b38191871 . _:b38191945 . _:b572937944 "2"^^ . _:b572937620 . _:b38191975 _:b38191992 . _:b572937847 . . _:b38191975 _:b38191993 . _:b572937671 . _:b572937534 . _:b38191946 . _:b572937947 "2"^^ . _:b572937537 "5"^^ . . _:b572937670 . _:b572937536 "5"^^ . _:b38191947 . _:b572937946 "2"^^ . . _:b38191844 . _:b38191895 . _:b572937760 . _:b38191975 _:b38191984 . . _:b38191975 _:b38191985 . . _:b572937952 . _:b572937665 . _:b38191975 _:b38191986 . _:b572937539 "5"^^ . _:b38191956 . _:b572937957 "2"^^ . _:b572937833 . _:b572937774 . _:b38191975 _:b38191987 . _:b38191860 "egfr-targeted therapies" . _:b38191894 . _:b38191975 _:b38191988 . _:b38191975 _:b38191989 . _:b38191975 _:b38191990 . _:b572937835 . _:b572937664 . _:b572937538 "5"^^ . _:b38191957 . _:b572937956 "2"^^ . _:b38191975 _:b38191991 . _:b38191975 _:b38191976 . _:b38191975 _:b38191977 . _:b572937549 "4"^^ . _:b572937667 . _:b38191975 _:b38191978 . _:b38191975 _:b38191979 . _:b38191958 . _:b38191975 _:b38191980 . _:b38191975 _:b38191981 . _:b572937548 "4"^^ . _:b572937666 . _:b38191975 _:b38191982 . . _:b572937517 . _:b38191959 . _:b572937958 "2"^^ . _:b38191975 _:b38191983 . _:b572937518 . _:b572937519 . _:b572937551 "4"^^ . _:b572937883 . _:b572937677 . _:b572937953 "2"^^ . _:b38191952 . . _:b38191852 . _:b572937550 "4"^^ . _:b572937676 . . _:b38191953 . _:b572937952 "2"^^ . . _:b572937634 . _:b572937528 . _:b572937679 . _:b572937545 "4"^^ . _:b572937529 . _:b38191954 . _:b572937955 "2"^^ . _:b572937530 . _:b38191896 . _:b572937531 . _:b572937532 . _:b572937678 . _:b572937544 "4"^^ . . _:b38191955 . _:b572937954 "2"^^ . _:b572937533 . _:b572937534 . . _:b572937535 . . _:b572937673 . _:b572937547 "4"^^ . _:b572937520 . _:b572937521 . _:b38191964 . _:b572937522 . _:b572937523 . . _:b572937524 . _:b572937672 . _:b572937546 "4"^^ . _:b572937525 . _:b38191965 . _:b38191848 "Primary GBM is frequently associated with epidermal growth factor receptor (EGFR) amplifications compared with secondary GBM, which may arise from lower grade precursors (>>5<<, 9)." . . _:b572937526 . _:b38191946 . . _:b572937527 . _:b572937779 . . _:b572937675 . . _:b572937557 "4"^^ . _:b38191966 . _:b572937556 "4"^^ . _:b572937674 . _:b38191967 . . _:b572937559 "4"^^ . _:b572937685 . _:b38191960 . . . _:b572937558 "4"^^ . _:b572937684 . _:b38191961 . . _:b572937553 "4"^^ . _:b572937687 . _:b38191962 . . _:b38191957 . _:b572937759 . _:b572937552 "4"^^ . _:b572937686 . _:b572937660 . _:b38191963 . _:b572937555 "4"^^ . _:b572937681 . _:b572937640 . _:b38191972 . . . . _:b572937554 "4"^^ . _:b572937680 . _:b38191973 . _:b572937535 . _:b572937630 . _:b572937576 . _:b572937683 . _:b572937565 "4"^^ . _:b572937577 . _:b38191974 . _:b572937578 . . _:b572937579 . _:b572937580 . _:b38191845 . _:b572937682 . _:b572937564 "4"^^ . _:b572937581 . _:b38191975 . _:b572937582 . _:b572937583 . _:b572937629 . . _:b572937568 . _:b572937693 . _:b572937567 "4"^^ . _:b572937569 . _:b38191968 . . _:b572937570 . . _:b572937571 . _:b572937572 . _:b572937692 . _:b572937566 "4"^^ . _:b572937573 . _:b38191969 . _:b572937811 . _:b572937574 . _:b572937775 . . . _:b572937575 . _:b572937592 . _:b572937695 . _:b572937561 "4"^^ . _:b572937593 . _:b38191974 . _:b38191970 . _:b572937594 . _:b38191943 . _:b572937595 . _:b572937596 . _:b572937764 . _:b572937694 . _:b572937560 "4"^^ . _:b572937597 . _:b38191971 . _:b38191898 "EGFRvIII), when compared to wtEGFR cell lines (>>28<<\u201330). This growth advantage is thought to result from an elevated proliferation rate coupled with a reduction in apoptosis (28)." . _:b572937598 . _:b572937599 . _:b572937584 . _:b572937689 . _:b572937563 "4"^^ . _:b572937585 . _:b38191980 . _:b38191924 "Pre-clinical results demonstrate the ability of TKIs to inhibit tumor cell growth, angiogenesis, survival, and proliferation in several different EGFR transfected GBM cell lines (36, >>49<<, 53, 54). However, these results do not appear to be clinically translatable, as response rates in GBM patients are disappointing for many inhibitors including gefitinib and erlotinib (55, 56)." . _:b572937586 . _:b572937587 . . _:b572937688 . _:b572937562 "4"^^ . _:b572937588 . _:b572937589 . _:b38191981 . _:b572937705 . _:b572937590 . _:b572937748 . _:b572937591 . _:b572937573 "3"^^ . _:b572937544 . _:b572937691 . . _:b572937545 . _:b38191982 . _:b572937546 . _:b572937547 . _:b572937572 "3"^^ . _:b572937548 . _:b572937949 . _:b572937690 . _:b572937549 . _:b38191983 . _:b572937550 . . _:b572937551 . . _:b572937575 "3"^^ . _:b572937536 . _:b572937701 . . . _:b572937537 . _:b38191976 . _:b572937538 . _:b38191935 "The unconjugated antibodies bind the extracellular domain of EGFR, and they are also suggested to cause internalization of EGFRvIII, though clinical trials have had varying results (>>48<<)." . _:b572937891 . _:b572937539 . _:b572937574 "3"^^ . _:b572937540 . _:b572937700 . _:b572937541 . _:b38191977 . . _:b572937542 . _:b38191949 _:b38191952 . . _:b572937543 . _:b38191949 _:b38191953 . _:b572937569 "3"^^ . _:b572937560 . _:b572937703 . _:b38191949 _:b38191954 . . _:b572937561 . _:b38191978 . _:b38191949 _:b38191955 . _:b572937562 . _:b38191949 _:b38191956 . _:b572937563 . _:b38191949 _:b38191957 . _:b572937568 "3"^^ . _:b572937564 . _:b572937702 . _:b572937565 . _:b38191979 . _:b572937566 . _:b572937567 . _:b572937936 . _:b572937637 . _:b572937571 "3"^^ . _:b572937552 . _:b572937697 . _:b572937553 . _:b38191988 . _:b572937554 . _:b572937555 . _:b572937570 "3"^^ . _:b572937556 . _:b572937696 . _:b572937557 . _:b38191989 . _:b572937558 . _:b572937559 . _:b572937581 "3"^^ . _:b572937640 . _:b572937699 . _:b572937641 . _:b38191990 . _:b572937642 . _:b572937822 . . _:b572937643 . _:b572937580 "3"^^ . _:b572937644 . _:b572937698 . _:b572937645 . _:b38191991 . _:b38191884 "mTORC2 activation results in phosphorylation and subsequent activation of several molecules (including AKT) that are involved in cell survival, metabolism, and proliferation (>>22<<). PTEN is a major inhibitor of this pathway by preventing phosphorylation of PIP2 to PIP3 (22). Loss of PTEN, such as the homozygous deletions observed in around 36% of gliomas, results in dramatic up-regulation of this pathway, and may" . _:b572937646 . _:b572937647 . _:b572937583 "3"^^ . _:b572937632 . _:b38191897 "A retrospective analysis of clinical trials found that of 40 patients with EGFR amplification, those also expressing EGFRvIII had significantly shorter survival (0.839\u2009years) than patients without (1.374\u2009years), P\u2009=\u20090.0031 (>>13<<). Considerable growth advantage has been observed in EGFRvIII transfected GBM cell lines (U87MG." . _:b572937709 . _:b572937633 . _:b38191984 . _:b38191917 . . _:b572937634 . _:b38191904 "ASPM promotes neural stem cell self-proliferation and hence it has been postulated the increased expression results in enhanced GBM progression by promoting cancer stem cell self-renewal (>>34<<)." . _:b572937635 . _:b38191847 . _:b572937582 "3"^^ . _:b572937636 . _:b572937708 . _:b38191949 _:b38191950 . . _:b572937637 . _:b38191985 . _:b38191949 _:b38191951 . _:b572937638 . . _:b572937639 . _:b572937577 "3"^^ . _:b572937656 . _:b572937711 . _:b572937657 . _:b38191986 . _:b572937658 . . _:b572937659 . _:b572937576 "3"^^ . _:b572937660 . _:b572937710 . . _:b38191987 . _:b38191859 "More recently, interest has focused on an anti-EGFRvIII vaccine (known as rindopepimut), which has entered clinical trials (>>19<<)." . _:b572937942 . _:b572937661 . _:b572937662 . _:b38191934 "Conjugated and unconjugated antibodies have been developed to target both wtEGFR and EGFRvIII, the most successful so far being cetuximab, panitumumab, and nimotuzumab (41, >>47<<). The unconjugated antibodies bind the extracellular domain of EGFR, and they are also suggested to cause internalization of EGFRvIII, though clinical trials have had varying results (48)." . _:b572937663 . _:b38191952 . _:b572937579 "3"^^ . _:b572937648 . _:b572937705 . _:b572937669 . _:b572937649 . _:b572937650 . _:b572937884 . _:b572937651 . _:b572937578 "3"^^ . _:b572937572 . _:b572937704 . . _:b572937652 . _:b572937653 . _:b572937654 . _:b572937655 . _:b572937584 . _:b572937589 "3"^^ . _:b572937608 . _:b38191992 "study, Nathanson et al. also described a reversal of erlotinib resistance within 72\u2009h upon withdrawal of the drug, where extrachromosomal EGFRvIII DNA was dramatically upregulated, and restored sensitivity to TKI-induced cell death (>>92<<)." . _:b572937707 . _:b572937609 . _:b572937610 . _:b572937574 . _:b572937611 . _:b572937588 "3"^^ . _:b572937612 . _:b572937706 . . _:b572937613 . _:b572937614 . _:b572937615 . _:b572937591 "3"^^ . _:b572937600 . _:b572937717 . _:b572937601 . _:b38191992 . . _:b38191901 "Similar to EGFR signaling, EGFRvIII activates the RTK/RAS/PI3K pathway as a result of EGFRvIII expression (31, >>32<<). This results in increased levels of phosphorylated AKT and reduced levels of P27KIP1, a cell cycle regulator that inhibits G1\u2013S phase transition in cell lines (33). Furthermore, abnormal spindle-like microcephaly-associated (ASPM)" . _:b572937602 . . _:b572937590 "3"^^ . _:b572937603 . _:b572937604 . _:b572937716 . _:b572937605 . _:b38191993 . _:b572937606 . _:b38191968 . _:b572937607 . _:b572937585 "3"^^ . . _:b572937719 . _:b572937624 . _:b572937625 . . _:b572937626 . _:b572937627 . _:b572937584 "3"^^ . _:b572937628 . _:b572937718 . _:b572937629 . _:b572937630 . _:b572937631 . _:b572937587 "3"^^ . _:b572937616 . _:b572937713 . _:b572937617 . _:b572937618 . _:b572937619 . . _:b572937586 "3"^^ . _:b572937620 . _:b572937712 . _:b572937621 . _:b572937676 . . _:b572937622 . _:b572937624 . _:b572937623 . _:b572937597 "3"^^ . _:b572937704 . _:b572937715 . . _:b572937705 . _:b572937706 . _:b572937707 . _:b572937548 . _:b572937596 "3"^^ . _:b572937708 . _:b572937714 . _:b572937709 . _:b572937710 . _:b38191958 _:b38191972 . _:b572937711 . _:b38191958 _:b38191973 . _:b572937599 "3"^^ . _:b572937696 . _:b572937725 . _:b572937672 . _:b38191958 _:b38191974 . . _:b572937697 . _:b572937698 . _:b38191932 . _:b38191958 _:b38191968 . _:b572937699 . _:b38191958 _:b38191969 . _:b572937598 "3"^^ . _:b572937700 . _:b572937724 . _:b38191958 _:b38191970 . . _:b572937701 . _:b38191958 _:b38191971 . _:b572937702 . _:b38191958 _:b38191964 . _:b572937703 . _:b38191958 _:b38191965 . . _:b572937593 "3"^^ . . _:b572937727 . _:b38191958 _:b38191966 . _:b572937720 . _:b572937721 . _:b38191931 . _:b38191958 _:b38191967 . _:b572937722 . _:b38191958 _:b38191960 . _:b572937723 . _:b38191958 _:b38191961 . _:b572937592 "3"^^ . _:b572937724 . _:b572937726 . _:b38191958 _:b38191962 . . _:b38191958 _:b38191963 . _:b572937725 . _:b572937726 . _:b572937727 . _:b572937595 "3"^^ . _:b572937712 . _:b572937721 . _:b572937713 . _:b38191958 _:b38191959 . _:b572937714 . _:b572937715 . _:b572937594 "3"^^ . _:b572937716 . _:b38191918 . _:b572937720 . . _:b572937717 . _:b572937718 . _:b572937719 . _:b572937605 "3"^^ . _:b572937672 . _:b572937723 . _:b572937722 . _:b572937673 . _:b572937904 . _:b572937674 . _:b38191984 "could be selected for, maintaining the tumor population and allowing functional resistance to EGFR-targeting therapies, and inhibitors of other ERBB family members may also be required for down-regulation of downstream elements (86, >>91<<)." . _:b572937675 . _:b38191988 "This uncoupling of the downstream pathways from EGFR signaling could be a possible explanation for the poor clinical responses exhibited with TKIs such as erlotinib (>>88<<)." . _:b572937604 "3"^^ . _:b572937676 . _:b572937722 . . _:b572937677 . _:b572937678 . _:b572937646 . . _:b572937607 "3"^^ . _:b572937679 . _:b572937664 . _:b572937554 . _:b572937665 . _:b572937666 . _:b572937667 . _:b572937606 "3"^^ . _:b572937668 . _:b572937669 . _:b572937670 . _:b572937839 . _:b572937671 . _:b572937601 "3"^^ . _:b572937688 . . . _:b572937689 . _:b572937690 . _:b572937691 . _:b572937622 . _:b572937600 "3"^^ . _:b572937692 . _:b572937693 . _:b572937580 . _:b572937694 . . _:b572937695 . _:b572937603 "3"^^ . _:b572937680 . _:b572937681 . _:b572937682 . _:b572937683 . _:b38191940 "clinical trials involving similar antibody-based therapies have been equally unsuccessful, though a decrease in skin toxicity has been reported with use of nimotuzumab, which could increase its viability as an adjuvant therapy in GBM (>>47<<)." . _:b572937758 . _:b572937602 "3"^^ . _:b572937684 . _:b572937685 . _:b572937686 . _:b38191983 "could be selected for, maintaining the tumor population and allowing functional resistance to EGFR-targeting therapies, and inhibitors of other ERBB family members may also be required for down-regulation of downstream elements (>>86<<, 91)." . _:b572937687 . _:b572937613 "3"^^ . _:b572937768 . _:b572937679 . _:b572937769 . _:b572937770 . _:b572937771 . _:b572937612 "3"^^ . _:b572937772 . . _:b572937773 . . _:b572937774 . _:b572937775 . _:b572937615 "3"^^ . _:b572937760 . _:b572937761 . _:b38191915 "In the monotherapy group, 11 patients had CNS metastases compared with 4 in the combination therapy group (44), though lapatinib has not yet been shown to have activity against recurrent GBM in clinical trials (>>45<<)." . _:b572937762 . . _:b572937614 "3"^^ . . _:b572937763 . _:b572937764 . _:b572937765 . _:b38191852 "In addition, around 50% of patients with EGFR amplification harbor a specific mutation \u2013 known as EGFRvIII \u2013 which results from an in-frame deletion of exons 2\u20137 (11, >>12<<). EGFRvIII can also be present independently of EGFR amplification (13)." . _:b572937766 . . _:b572937767 . _:b572937609 "3"^^ . _:b572937784 . _:b572937785 . _:b572937786 . _:b572937787 . _:b572937849 . _:b572937608 "3"^^ . _:b572937788 . _:b572937789 . _:b572937790 . _:b572937791 . _:b572937611 "3"^^ . _:b572937776 . _:b572937777 . . . _:b572937778 . _:b572937779 . _:b572937610 "3"^^ . . _:b572937780 . _:b572937544 . _:b572937781 . _:b572937782 . _:b572937638 . _:b38191930 _:b38191932 . _:b572937783 . _:b572937621 "3"^^ . _:b38191930 _:b38191933 . _:b572937736 . _:b38191930 _:b38191934 . _:b572937737 . _:b38191930 _:b38191935 . _:b572937738 . _:b38191872 . _:b572937739 . _:b572937888 . _:b572937620 "3"^^ . _:b572937740 . _:b572937957 . _:b572937741 . _:b572937701 . _:b38191930 _:b38191931 . _:b572937742 . _:b572937743 . _:b572937625 . _:b572937623 "3"^^ . _:b572937728 . _:b38191876 "egfr signaling in gbm" . _:b38191941 "In a Phase III study, patients were administered nimotuzumab with concurrent radiotherapy (>>47<<). Although there was no statistically significant difference in overall survival of patients, the patients with the greatest median overall survival had molecular markers of EGFR amplification and unmethylated MGMT (47)." . _:b38191905 "The proliferative effects of EGFRvIII may be potentiated by the anti-apoptotic nature of brain tissue through up-regulation of Bcl-XL, which is a potent inhibitor of apoptosis (>>35<<). EGFRvIII has also been shown to have a role in tumor invasiveness, for example, in vitro studies demonstrated that U87MG." . _:b572937729 . _:b572937730 . _:b572937731 . _:b572937697 . _:b572937622 "3"^^ . _:b572937732 . _:b572937733 . _:b572937734 . _:b38191909 _:b38191920 . _:b38191930 _:b38191948 . . . _:b572937735 . _:b572937617 "3"^^ . _:b38191909 _:b38191921 . _:b572937530 . _:b572937752 . _:b38191909 _:b38191922 . _:b572937753 . _:b38191909 _:b38191923 . . _:b572937754 . _:b572937910 . _:b38191930 _:b38191944 . _:b38191909 _:b38191924 . _:b572937755 . _:b572937616 "3"^^ . _:b38191930 _:b38191945 . _:b38191909 _:b38191925 . _:b572937756 . _:b38191930 _:b38191946 . _:b38191909 _:b38191926 . _:b572937757 . _:b38191930 _:b38191947 . _:b38191909 _:b38191927 . _:b572937758 . _:b38191874 "cases, examining tumoral heterogeneity via immunohistochemistry found that in one case the over-expression and amplification were localized to one half of the glioma, with the other half demonstrating normal levels of EGFR expression (>>85<<). Furthermore, not all of the samples with genetic amplifications also had increased levels of RNA, which further complicates the assessment of the viability of using EGFR-targeted therapies (85)." . _:b38191930 _:b38191940 . _:b38191909 _:b38191928 . . _:b572937759 . _:b572937724 . _:b572937619 "3"^^ . _:b38191930 _:b38191941 . _:b38191909 _:b38191929 . . . . _:b38191930 _:b38191942 . _:b572937744 . _:b572937745 . _:b38191930 _:b38191943 . _:b572937746 . _:b38191930 _:b38191936 . _:b572937747 . _:b38191963 "Antisense RNA appears to be efficacious in targeting EGFR expressing cells in vitro (70, >>72<<, 73). Injection of vectors containing antisense RNA to target EGFRvIII into intracranial glioblastoma xenografts were found to reduce tumor volume by >40-fold compared with controls (74)." . _:b38191857 "Although EGFR kinase inhibitor therapy has shown initial success in other cancers such non-small cell lung cancer (>>15<<), previous trials in glioblastoma have been unsuccessful to date (16\u201318)." . _:b572937618 "3"^^ . _:b38191930 _:b38191937 . _:b572937748 . _:b38191930 _:b38191938 . _:b572937749 . _:b38191930 _:b38191939 . _:b572937750 . _:b38191981 "Additionally, in GBM there is a redundancy in activation of PI3K due to the availability of several types of tyrosine kinases upstream (86), including GFR1, MET, PDGFR\u03B1/\u03B2, and uPAR (89, >>90<<). Increased activation of other members of the ERBB family of tyrosine kinases has also been described, as compensatory activation of ERBB2 and ERBB3 was noted after EGFR withdrawal in GBM cancer-stem-cell lines (91)." . _:b38191845 . _:b572937827 . _:b572937751 . _:b572937629 "3"^^ . _:b572937832 . _:b572937833 . _:b572937877 . _:b572937834 . _:b572937835 . _:b572937628 "3"^^ . . _:b38191909 _:b38191910 . _:b572937836 . _:b572937837 . _:b38191909 _:b38191911 . _:b572937838 . _:b38191909 _:b38191912 . _:b38191891 "egfrviii signaling" . _:b572937839 . _:b572937631 "3"^^ . _:b38191909 _:b38191913 . _:b572937824 . _:b38191909 _:b38191914 . _:b572937825 . _:b38191909 _:b38191915 . _:b572937826 . _:b38191961 . _:b572937690 . _:b38191909 _:b38191916 . _:b572937827 . _:b572937630 "3"^^ . _:b38191909 _:b38191917 . . _:b572937828 . _:b38191909 _:b38191918 . . _:b572937829 . _:b572937947 . _:b38191909 _:b38191919 . _:b572937545 . _:b572937830 . _:b572937831 . _:b572937625 "3"^^ . _:b572937848 . _:b572937849 . . _:b38191967 . _:b572937850 . _:b572937851 . _:b572937624 "3"^^ . _:b572937594 . _:b572937852 . . _:b572937853 . . _:b572937854 . _:b572937855 . _:b572937627 "3"^^ . _:b572937573 . _:b572937840 . _:b572937790 . _:b572937841 . _:b572937842 . _:b38191965 . _:b38191864 "RNA therapies will also be discussed, which involve creating antisense oligonucleotides or siRNA complementary to the regions that it would be clinically beneficial to silence (>>40<<)." . _:b572937843 . _:b572937626 "3"^^ . . _:b572937844 . _:b572937845 . _:b572937846 . _:b572937847 . _:b572937637 "3"^^ . _:b572937800 . _:b572937801 . _:b572937802 . _:b572937803 . _:b572937636 . _:b572937636 "3"^^ . _:b572937804 . _:b572937805 . _:b38191909 "tyrosine kinase inhibitors targeting egfr" . _:b572937806 . _:b572937807 . _:b572937815 . _:b572937639 "3"^^ . _:b572937792 . _:b572937793 . _:b572937794 . _:b38191903 "EGFRvIII cells compared to parental U87MG cells (>>34<<). ASPM promotes neural stem cell self-proliferation and hence it has been postulated the increased expression results in enhanced GBM progression by promoting cancer stem cell self-renewal (34)." . _:b572937639 . _:b572937795 . _:b572937638 "3"^^ . _:b572937796 . _:b38191975 "gbm resistance to egfr inhibition" . _:b572937797 . _:b572937798 . _:b572937929 . _:b572937799 . _:b572937633 "3"^^ . _:b572937816 . _:b572937817 . _:b572937818 . _:b572937819 . _:b572937632 "3"^^ . _:b572937820 . _:b572937821 . _:b572937592 . _:b38191891 _:b38191904 . . _:b572937822 . . _:b38191891 _:b38191905 . _:b572937635 "3"^^ . . _:b572937823 . _:b38191891 _:b38191906 . _:b572937887 . _:b572937808 . _:b38191891 _:b38191907 . _:b38191975 . _:b572937809 . _:b38191891 _:b38191908 . _:b572937810 . . _:b572937811 . _:b572937634 "3"^^ . . _:b572937812 . _:b572937730 . _:b572937813 . _:b572937746 . _:b572937814 . _:b572937815 . _:b572937645 "3"^^ . . _:b572937896 . _:b572937897 . _:b572937898 . _:b572937899 . _:b572937644 "3"^^ . _:b572937900 . _:b572937570 . . _:b572937901 . _:b572937523 . _:b572937902 . _:b572937903 . _:b572937647 "3"^^ . . _:b38191942 "Although there was no statistically significant difference in overall survival of patients, the patients with the greatest median overall survival had molecular markers of EGFR amplification and unmethylated MGMT (>>47<<)." . _:b38191958 . _:b572937888 . _:b572937889 . _:b572937610 . _:b572937890 . _:b572937891 . _:b572937646 "3"^^ . _:b572937892 . _:b572937893 . _:b572937894 . _:b572937895 . _:b38191845 . _:b572937641 "3"^^ . _:b38191949 . _:b572937912 . _:b38191889 . _:b572937913 . _:b572937585 . _:b572937914 . _:b38191919 . _:b572937915 . _:b38191902 . _:b572937640 "3"^^ . _:b572937916 . _:b572937917 . _:b572937729 . . _:b572937918 . _:b572937919 . _:b572937643 "3"^^ . _:b572937904 . _:b572937876 . _:b572937905 . _:b572937906 . _:b572937907 . _:b572937642 "3"^^ . _:b572937908 . _:b38191930 . _:b572937909 . _:b572937662 . _:b38191891 _:b38191896 . _:b572937653 "2"^^ . _:b572937910 . _:b38191891 _:b38191897 . _:b572937911 . _:b38191891 _:b38191898 . _:b572937864 . _:b38191891 _:b38191899 . _:b572937865 . _:b38191891 _:b38191900 . _:b572937652 "2"^^ . _:b572937866 . _:b38191891 _:b38191901 . _:b572937867 . _:b38191891 _:b38191902 . _:b572937868 . _:b38191891 _:b38191903 . _:b572937871 . _:b572937869 . . _:b572937870 . _:b572937655 "2"^^ . . _:b572937871 . _:b572937856 . _:b38191854 "The other critically important pathways that regulate cell proliferation and survival are p53 and RB signaling (>>5<<) [adapted from Ref." . _:b572937857 . _:b572937527 . _:b38191891 _:b38191892 . _:b572937654 "2"^^ . _:b572937858 . _:b38191891 _:b38191893 . _:b572937859 . _:b38191891 _:b38191894 . _:b572937860 . _:b38191891 _:b38191895 . _:b572937861 . _:b572937862 . _:b572937863 . _:b572937649 "3"^^ . _:b572937628 . _:b572937880 . . _:b572937881 . _:b572937819 . . _:b572937882 . _:b572937883 . _:b572937648 "3"^^ . _:b572937884 . _:b572937726 . _:b572937885 . _:b572937886 . _:b572937651 "2"^^ . _:b572937887 . _:b38191909 . _:b572937872 . _:b572937873 . _:b572937674 . _:b572937874 . _:b572937875 . _:b572937650 "3"^^ . _:b572937876 . _:b572937877 . _:b572937878 . _:b572937661 "2"^^ . _:b572937879 . _:b572937691 . . _:b572937660 "2"^^ . . _:b572937517 . _:b38191891 . _:b572937663 "2"^^ . _:b572937952 . _:b572937953 . _:b572937864 . _:b572937954 . _:b572937662 "2"^^ . _:b572937955 . _:b38191863 "The current immunotherapy for EGFRvIII can be administered in the form of an intradermal vaccine CDX-110 and granulocyte macrophage-colony stimulating factor (GM-CSF) (>>39<<). RNA therapies will also be discussed, which involve creating antisense oligonucleotides or siRNA complementary to the regions that it would be clinically beneficial to silence (40)." . _:b572937934 . _:b572937956 . _:b38191938 . _:b572937957 . _:b572937958 . _:b572937657 "2"^^ . _:b572937852 . _:b38191928 "However, these results do not appear to be clinically translatable, as response rates in GBM patients are disappointing for many inhibitors including gefitinib and erlotinib (55, >>56<<). One explanation of this could be that TKIs are most efficacious when targeting tumor cells that express mutations in exons 19 and 21 of the EGFR kinase domain, which has been identified in various cancer types but has not yet been" . _:b572937922 . _:b38191939 . _:b38191876 _:b38191888 . _:b572937785 . _:b572937667 . _:b572937656 "2"^^ . _:b38191876 _:b38191889 . _:b572937696 . _:b572937598 . . _:b38191876 _:b38191890 . _:b38191991 "Erlotinib resistance appears to be linked to EGFRvIII suppression in extrachromosomal DNA in order to successfully evade therapeutic mechanisms that target extrachromosomal oncogenes (>>92<<). Surprisingly, in this study, Nathanson et al. also described a reversal of erlotinib resistance within 72\u2009h upon withdrawal of the drug, where extrachromosomal EGFRvIII DNA was dramatically upregulated, and restored sensitivity to" . _:b38191986 "in expression of other ERBB receptors may render EGFR signaling dispensable in the tumor, allowing growth and survival without EGFR signaling and thus negating the therapeutic viability of EGFR-targeting therapies in these cases (87, >>88<<, 91). This uncoupling of the downstream pathways from EGFR signaling could be a possible explanation for the poor clinical responses exhibited with TKIs such as erlotinib (88)." . . _:b38191876 . _:b572937659 "2"^^ . . . _:b572937658 "2"^^ . _:b38191845 . . _:b572937669 "2"^^ . _:b38191876 _:b38191877 . . _:b38191876 _:b38191878 . _:b572937928 . _:b38191876 _:b38191879 . _:b572937929 . _:b572937930 . _:b572937668 "2"^^ . _:b572937931 . _:b572937932 . _:b572937933 . _:b572937850 . _:b572937832 . . _:b38191876 _:b38191884 . _:b572937671 "2"^^ . _:b572937934 . . _:b38191876 _:b38191885 . _:b572937627 . _:b572937935 . _:b38191876 _:b38191886 . _:b572937920 . _:b38191876 _:b38191887 . _:b572937921 . _:b38191876 _:b38191880 . _:b572937670 "2"^^ . _:b572937922 . _:b38191876 _:b38191881 . _:b572937897 . _:b572937923 . _:b38191876 _:b38191882 . _:b572937800 . _:b572937924 . _:b38191876 _:b38191883 . _:b572937925 . _:b572937926 . _:b572937665 "2"^^ . _:b572937927 . _:b572937944 . _:b572937945 . _:b572937946 . _:b38191859 . _:b572937664 "2"^^ . _:b572937947 . _:b572937651 . _:b572937948 . _:b572937536 . . _:b572937949 . _:b572937950 . _:b572937667 "2"^^ . _:b572937951 . . _:b572937936 . _:b572937937 . . _:b38191844 "introduction" . _:b572937666 "2"^^ . _:b572937938 . . _:b572937939 . _:b38191940 . _:b38191929 "of this could be that TKIs are most efficacious when targeting tumor cells that express mutations in exons 19 and 21 of the EGFR kinase domain, which has been identified in various cancer types but has not yet been elucidated in GBM (>>14<<)." . _:b572937940 . . . _:b572937941 . _:b572937942 . _:b572937677 "2"^^ . _:b572937943 . _:b38191941 . _:b572937617 . . . _:b572937713 . _:b572937676 "2"^^ . _:b38191942 . . _:b572937679 "2"^^ . _:b38191948 "conjugated to death receptor agonists have been reported to induce apoptosis in GBM cell lines, for example, the scFvM58\u2013sTRAIL fusion, which has been shown to selectively target GBM cells that express multidrug resistance protein 3 (>>62<<)." . . _:b572937685 . _:b572937678 "2"^^ . _:b572937841 . _:b572937673 "2"^^ . _:b38191873 "Furthermore, RNA entities are anionic, hydrophilic, and unable to enter cell by passive diffusion mechanisms, so the BBB is essentially impenetrable to any potentially therapeutic RNA molecules (>>84<<)." . _:b572937672 "2"^^ . . _:b572937955 . _:b572937675 "2"^^ . _:b38191853 . _:b572937674 "2"^^ . _:b572937751 . _:b572937685 "2"^^ . _:b38191987 . _:b572937684 "2"^^ . . . _:b572937687 "2"^^ . _:b572937866 . . _:b38191927 "However, these results do not appear to be clinically translatable, as response rates in GBM patients are disappointing for many inhibitors including gefitinib and erlotinib (>>55<<, 56). One explanation of this could be that TKIs are most efficacious when targeting tumor cells that express mutations in exons 19 and 21 of the EGFR kinase domain, which has been identified in various cancer types but has not yet been" . _:b572937686 "2"^^ . _:b38191946 "Various early clinical trials report that administering the radiolabeled antibody 125I-MAb 425 intravenously, either alone or with standard of care treatment, significantly improves median survival (38, 50, >>61<<). In the largest Phase II trial to date combined treatment of 125I-mAb 425 and TMZ provided the greatest survival benefit with a median survival of 20.4\u2009months, compared to treatment of 125I-mAb 425 alone, which was 14.5\u2009months (50)." . _:b38191984 . . _:b572937681 "2"^^ . _:b572937664 . _:b572937882 . _:b572937680 "2"^^ . _:b572937551 . _:b572937615 . _:b572937683 "2"^^ . _:b572937817 . _:b572937806 . . _:b572937682 "2"^^ . _:b572937613 . _:b572937693 "2"^^ . . _:b572937692 "2"^^ . _:b38191989 . _:b572937739 . _:b38191985 "in expression of other ERBB receptors may render EGFR signaling dispensable in the tumor, allowing growth and survival without EGFR signaling and thus negating the therapeutic viability of EGFR-targeting therapies in these cases (>>87<<, 88, 91). This uncoupling of the downstream pathways from EGFR signaling could be a possible explanation for the poor clinical responses exhibited with TKIs such as erlotinib (88)." . _:b572937695 "2"^^ . _:b572937818 . _:b38191888 "Loss of PTEN, such as the homozygous deletions observed in around 36% of gliomas, results in dramatic up-regulation of this pathway, and may be a major source of resistance to EGFR therapies (5, 22, >>23<<)." . _:b572937694 "2"^^ . _:b572937743 . _:b572937821 . _:b572937689 "2"^^ . _:b572937518 . _:b38191879 "by EGF results in the activation of the RTK/RAS/PI(3)K pathway (21) via receptor phosphorylation, ultimately resulting in cellular proliferation, angiogenesis, and increased local tissue invasion as well as resistance to apoptosis (>>21<<\u201323)." . _:b38191881 "PI3K phosphorylates phosphatidylinositol 4,5-biphosphate (PIP2) to phosphatidylinositol 3,4,5-triphosphate (PIP3), which results in further activation of AKT (>>22<<). AKT then promotes activation of mTOR \u2013 a protein, which exists as two complexes; mTORC1 and mTORC2 (24). Activation of mTORC1 promotes cellular growth by biosynthesis of proteins, lipids, and organelles in addition to inhibition of" . _:b572937688 "2"^^ . . . . _:b572937691 "2"^^ . . _:b38191872 "In RNA studies, AAV/shRNA vectors were found to be severely toxic, and caused fatality in 64% of mice due to oversaturation of RNAi pathways (82, >>83<<). Furthermore, RNA entities are anionic, hydrophilic, and unable to enter cell by passive diffusion mechanisms, so the BBB is essentially impenetrable to any potentially therapeutic RNA molecules (84)." . _:b572937838 . _:b572937906 . _:b572937648 . _:b572937690 "2"^^ . _:b38191862 . _:b572937701 "2"^^ . _:b572937712 . . _:b572937700 "2"^^ . . _:b572937740 . . . . _:b572937703 "2"^^ . . _:b572937734 . _:b572937702 "2"^^ . _:b572937769 . . _:b572937697 "2"^^ . _:b572937642 . _:b572937914 . _:b572937696 "2"^^ . _:b38191855 "The other critically important pathways that regulate cell proliferation and survival are p53 and RB signaling (5) [adapted from Ref. (>>14<<)]." . _:b38191982 . _:b572937699 "2"^^ . . _:b572937698 "2"^^ . . _:b572937709 "2"^^ . . _:b572937919 . _:b572937708 "2"^^ . . _:b572937733 . _:b572937711 "2"^^ . _:b572937732 . _:b38191895 "It has been suggested that the transformation happens intracellularly, with EGFRvIII being phosphorylated in an EGFR-dependent manner leading to increased downstream STAT signaling (>>27<<). There is also evidence in GBM of EGFRvIII internalization to form an oncogenic complex with STAT3 (27)." . _:b572937710 "2"^^ . _:b572937735 . . _:b572937705 "2"^^ . _:b572937734 . _:b572937702 . _:b572937704 "2"^^ . _:b572937840 . _:b572937729 . _:b572937707 "2"^^ . _:b572937728 . _:b38191914 . _:b572937767 . . _:b572937706 "2"^^ . _:b572937519 . _:b572937731 . _:b38191849 "Primary GBM is frequently associated with epidermal growth factor receptor (EGFR) amplifications compared with secondary GBM, which may arise from lower grade precursors (5, >>9<<)." . _:b572937943 . _:b38191890 "In vitro studies suggest that the signaling mechanism of EGFRvIII cells can confer resistance to EGFR inhibitors (25), because EGFRvIII signals via an mTOR2 pathway whereas wtEGFR uses mTOR1 (>>24<<)." . _:b38191913 . _:b572937717 "2"^^ . _:b572937730 . _:b38191917 . . _:b38191947 "In the largest Phase II trial to date combined treatment of 125I-mAb 425 and TMZ provided the greatest survival benefit with a median survival of 20.4\u2009months, compared to treatment of 125I-mAb 425 alone, which was 14.5\u2009months (>>50<<). Antibodies conjugated to death receptor agonists have been reported to induce apoptosis in GBM cell lines, for example, the scFvM58\u2013sTRAIL fusion, which has been shown to selectively target GBM cells that express multidrug resistance" . . _:b572937716 "2"^^ . . _:b572937741 . _:b572937719 "2"^^ . _:b572937740 . _:b572937718 "2"^^ . _:b572937743 . _:b38191926 "Pre-clinical results demonstrate the ability of TKIs to inhibit tumor cell growth, angiogenesis, survival, and proliferation in several different EGFR transfected GBM cell lines (36, 49, 53, >>54<<). However, these results do not appear to be clinically translatable, as response rates in GBM patients are disappointing for many inhibitors including gefitinib and erlotinib (55, 56)." . _:b572937713 "2"^^ . _:b572937742 . . _:b572937712 "2"^^ . _:b38191847 . _:b572937737 . _:b38191993 "domain, whereas GBM EGFR mutations are mainly in the extracellular domain, which could allow the GBM mutant receptors sufficient flexibility within the kinase domain to accommodate lapatinib and other type II EGFR kinase inhibitors (>>93<<)." . _:b572937612 . _:b572937715 "2"^^ . _:b572937736 . _:b572937714 "2"^^ . _:b572937739 . _:b38191900 "Similar to EGFR signaling, EGFRvIII activates the RTK/RAS/PI3K pathway as a result of EGFRvIII expression (>>31<<, 32). This results in increased levels of phosphorylated AKT and reduced levels of P27KIP1, a cell cycle regulator that inhibits G1\u2013S phase transition in cell lines (33). Furthermore, abnormal spindle-like microcephaly-associated (ASPM)" . . _:b572937924 . . _:b572937725 "2"^^ . _:b572937738 . _:b572937724 "2"^^ . . _:b572937749 . _:b572937727 "2"^^ . _:b572937748 . _:b572937726 "2"^^ . _:b572937751 . _:b572937525 . _:b572937721 "2"^^ . _:b572937750 . . _:b572937720 "2"^^ . _:b572937745 . . . . _:b572937723 "2"^^ . _:b572937744 . _:b572937553 . _:b572937722 "2"^^ . _:b572937747 . _:b572937746 . _:b572937757 . _:b38191934 . _:b572937756 . _:b38191978 "Loss of PTEN has previously been found to be strongly correlated with treatment failure in GBM (87), to the extent that the analysis of EGFRvIII and PTEN levels may be used to predict tumor response to TKI therapy (>>88<<)." . _:b572937875 . . _:b572937759 . _:b38191861 "TKIs are small-molecule inhibitors, which bind to the ligand-binding site on the extracellular domain, and are the most clinically advanced EGFR-targeting therapy to date (>>14<<). Antibody-based therapy uses monoclonal antibodies that correspond to the receptor landscape to inhibit signaling, and can also use conjugated antibodies that allow toxins or radioactive isotopes to be targeted to specific cells (38)." . _:b572937758 . . _:b572937693 . _:b572937753 . . _:b572937895 . _:b572937752 . _:b572937781 . _:b572937755 . _:b38191976 "has been previously shown that targeting of the EGFR receptor can lead to selection pressure for somatic mutations at other points in the pathway, such as inactivating phosphorylation of PTEN, leading to resistance to EGFR inhibitors (>>86<<), which could present a further problem for the ACT IV trial." . _:b572937813 . _:b572937670 . _:b572937754 . _:b38191950 "In initial Phase I trials, vaccinations comprising dendritic cells (DCs) primed with EGFRvIII peptides were found to be safe, with only grade I and II skin reactions at the vaccine injection site reported (>>51<<). Patients were also found to be immunologically responsive when their cellular immune responses were tested regularly using skin tests (51)." . . _:b572937765 . _:b572937688 . . . _:b572937632 . _:b572937764 . _:b572937767 . _:b38191870 . . _:b572937766 . _:b572937644 . . _:b572937761 . _:b572937855 . _:b572937794 . _:b572937760 . . _:b38191938 "A Phase II study stratified patients depending on their EGFR gene amplification status and both groups were administered cetuximab intravenously (>>60<<). Cetuximab had little effect in both study groups and the median overall survival was 5\u2009months, eliciting no significant correlation between EGFR status and response or overall survival (60)." . _:b572937763 . . . . _:b572937762 . . _:b572937773 . . _:b38191869 "This is because it has been shown that in patients with tumor resection of <95% neither over-expression of wtEGFR nor the presence of EGFRvIII can be used independently to predict patient survival (>>81<<). One hypothesis is that a small tumor may still be relatively immunoprivileged; thus, the immune system may be relatively na\u00EFve to tumor antigens (39)." . _:b572937736 . _:b572937772 . . _:b572937775 . _:b572937948 . . _:b572937531 . _:b572937774 . _:b38191863 . _:b572937708 . . _:b38191966 . _:b572937656 . _:b572937769 . . . _:b572937768 . . . _:b572937771 . . _:b572937770 . . _:b572937567 . _:b572937781 . . _:b38191923 "Pre-clinical results demonstrate the ability of TKIs to inhibit tumor cell growth, angiogenesis, survival, and proliferation in several different EGFR transfected GBM cell lines (>>36<<, 49, 53, 54). However, these results do not appear to be clinically translatable, as response rates in GBM patients are disappointing for many inhibitors including gefitinib and erlotinib (55, 56)." . . _:b572937780 . _:b572937788 . _:b38191926 . . _:b38191903 . _:b572937783 . . _:b572937653 . . _:b572937663 . _:b572937782 . _:b572937777 .